Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 7.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_166 and HP15_167 are separated by 273 nucleotides HP15_167 and HP15_168 are separated by 672 nucleotides HP15_168 and HP15_169 are separated by 87 nucleotides
HP15_166: HP15_166 - cation efflux system protein, at 161,207 to 162,103
_166
HP15_167: HP15_167 - methionine-rich hypothetical protein, at 162,377 to 162,736
_167
HP15_168: HP15_168 - conserved hypothetical protein, at 163,409 to 163,513
_168
HP15_169: HP15_169 - hypothetical protein, at 163,601 to 163,714
_169
Position (kb)
162
163 Strain fitness (log2 ratio)
-2
-1
0
1 at 161.391 kb on + strand, within HP15_166 at 161.407 kb on - strand, within HP15_166 at 161.565 kb on + strand, within HP15_166 at 161.565 kb on + strand, within HP15_166 at 161.565 kb on + strand, within HP15_166 at 161.565 kb on + strand, within HP15_166 at 161.565 kb on + strand, within HP15_166 at 161.573 kb on - strand, within HP15_166 at 161.573 kb on - strand, within HP15_166 at 161.573 kb on - strand, within HP15_166 at 161.776 kb on + strand, within HP15_166 at 161.779 kb on - strand, within HP15_166 at 161.814 kb on - strand, within HP15_166 at 161.814 kb on - strand, within HP15_166 at 161.833 kb on + strand, within HP15_166 at 161.887 kb on + strand, within HP15_166 at 161.933 kb on + strand, within HP15_166 at 161.997 kb on + strand, within HP15_166 at 161.997 kb on + strand, within HP15_166 at 162.005 kb on - strand, within HP15_166 at 162.038 kb on - strand at 162.080 kb on - strand at 162.082 kb on + strand at 162.082 kb on + strand at 162.183 kb on - strand at 162.239 kb on + strand at 162.307 kb on + strand at 162.344 kb on + strand at 162.344 kb on + strand at 162.410 kb on + strand at 162.410 kb on + strand at 162.458 kb on - strand, within HP15_167 at 162.475 kb on - strand, within HP15_167 at 162.564 kb on + strand, within HP15_167 at 162.564 kb on + strand, within HP15_167 at 162.621 kb on - strand, within HP15_167 at 162.682 kb on - strand, within HP15_167 at 162.682 kb on - strand, within HP15_167 at 162.838 kb on + strand at 162.838 kb on + strand at 162.846 kb on - strand at 162.925 kb on + strand at 162.925 kb on + strand at 162.925 kb on + strand at 162.925 kb on + strand at 162.925 kb on + strand at 162.933 kb on - strand at 162.967 kb on - strand at 162.987 kb on + strand at 163.301 kb on - strand at 163.327 kb on + strand at 163.327 kb on + strand at 163.327 kb on + strand at 163.334 kb on + strand at 163.334 kb on + strand at 163.335 kb on - strand at 163.335 kb on - strand at 163.335 kb on - strand at 163.342 kb on - strand at 163.352 kb on - strand at 163.459 kb on + strand, within HP15_168 at 163.460 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.478 kb on + strand, within HP15_168 at 163.478 kb on - strand, within HP15_168 at 163.508 kb on + strand at 163.516 kb on - strand at 163.562 kb on + strand at 163.562 kb on + strand at 163.562 kb on + strand at 163.570 kb on - strand at 163.570 kb on - strand at 163.606 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 7.5 mM remove 161,391 + HP15_166 0.21 -0.5 161,407 - HP15_166 0.22 -0.5 161,565 + HP15_166 0.40 -0.0 161,565 + HP15_166 0.40 +0.6 161,565 + HP15_166 0.40 -0.2 161,565 + HP15_166 0.40 +0.4 161,565 + HP15_166 0.40 -1.8 161,573 - HP15_166 0.41 -0.1 161,573 - HP15_166 0.41 -0.2 161,573 - HP15_166 0.41 -1.4 161,776 + HP15_166 0.63 -0.4 161,779 - HP15_166 0.64 -0.5 161,814 - HP15_166 0.68 +1.3 161,814 - HP15_166 0.68 +0.2 161,833 + HP15_166 0.70 -1.0 161,887 + HP15_166 0.76 +1.0 161,933 + HP15_166 0.81 +1.0 161,997 + HP15_166 0.88 -0.0 161,997 + HP15_166 0.88 -0.7 162,005 - HP15_166 0.89 +0.1 162,038 - +0.2 162,080 - +0.4 162,082 + -0.2 162,082 + -0.5 162,183 - -0.1 162,239 + -1.7 162,307 + -0.4 162,344 + +0.3 162,344 + +0.2 162,410 + -0.7 162,410 + -0.8 162,458 - HP15_167 0.23 +1.3 162,475 - HP15_167 0.27 +0.6 162,564 + HP15_167 0.52 +0.2 162,564 + HP15_167 0.52 +1.7 162,621 - HP15_167 0.68 +0.8 162,682 - HP15_167 0.85 -0.2 162,682 - HP15_167 0.85 +0.5 162,838 + -1.0 162,838 + +0.0 162,846 - +0.1 162,925 + -0.1 162,925 + +0.2 162,925 + +0.0 162,925 + +0.5 162,925 + -0.3 162,933 - -0.5 162,967 - +0.2 162,987 + +1.3 163,301 - -0.1 163,327 + +0.4 163,327 + +0.5 163,327 + +0.2 163,334 + +1.0 163,334 + -0.8 163,335 - +0.6 163,335 - -0.1 163,335 - +0.2 163,342 - -0.1 163,352 - +0.5 163,459 + HP15_168 0.48 +0.4 163,460 + HP15_168 0.49 -0.3 163,471 + HP15_168 0.59 +0.0 163,471 + HP15_168 0.59 +0.2 163,471 + HP15_168 0.59 +0.5 163,478 + HP15_168 0.66 -0.2 163,478 - HP15_168 0.66 -0.1 163,508 + -0.8 163,516 - +0.7 163,562 + -0.2 163,562 + +0.9 163,562 + -0.1 163,570 - -0.8 163,570 - +0.5 163,606 + -1.7
Or see this region's nucleotide sequence