Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and purN overlap by 1 nucleotides purN and SO2762 are separated by 140 nucleotides
SO2760: purM - phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 2,882,759 to 2,883,817
purM
SO2761: purN - phosphoribosylglycinamide formyltransferase (NCBI ptt file), at 2,883,817 to 2,884,461
purN
SO2762: SO2762 - nagD protein (NCBI ptt file), at 2,884,602 to 2,885,348
SO2762
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2882.854 kb on + strand at 2882.862 kb on - strand at 2882.883 kb on + strand, within purM at 2882.976 kb on + strand, within purM at 2883.151 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.507 kb on - strand, within purM at 2883.666 kb on + strand, within purM at 2883.731 kb on - strand at 2883.962 kb on - strand, within purN at 2884.223 kb on + strand, within purN at 2884.357 kb on - strand, within purN at 2884.493 kb on - strand at 2884.583 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.645 kb on + strand at 2884.746 kb on + strand, within SO2762 at 2884.810 kb on - strand, within SO2762 at 2884.890 kb on - strand, within SO2762 at 2884.957 kb on + strand, within SO2762 at 2885.340 kb on - strand at 2885.342 kb on + strand at 2885.343 kb on + strand at 2885.361 kb on + strand at 2885.424 kb on - strand at 2885.424 kb on - strand at 2885.430 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 2,882,854 + -3.0 2,882,862 - -3.7 2,882,883 + purM SO2760 0.12 -4.4 2,882,976 + purM SO2760 0.20 -1.7 2,883,151 - purM SO2760 0.37 -3.7 2,883,244 - purM SO2760 0.46 -5.5 2,883,244 - purM SO2760 0.46 -3.1 2,883,507 - purM SO2760 0.71 -4.7 2,883,666 + purM SO2760 0.86 -1.0 2,883,731 - -3.5 2,883,962 - purN SO2761 0.22 -0.8 2,884,223 + purN SO2761 0.63 -2.2 2,884,357 - purN SO2761 0.84 -1.8 2,884,493 - -0.0 2,884,583 + +0.2 2,884,630 + +1.0 2,884,630 + -0.5 2,884,630 + -0.0 2,884,630 + -0.2 2,884,645 + +0.3 2,884,746 + SO2762 0.19 +0.7 2,884,810 - SO2762 0.28 -1.4 2,884,890 - SO2762 0.39 -0.1 2,884,957 + SO2762 0.48 -0.1 2,885,340 - -0.5 2,885,342 + +0.1 2,885,343 + +0.7 2,885,361 + +0.5 2,885,424 - +0.3 2,885,424 - +0.1 2,885,430 + +0.0
Or see this region's nucleotide sequence