Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2095 and SO2096 overlap by 35 nucleotides SO2096 and hydC are separated by 7 nucleotides hydC and hyaB are separated by 21 nucleotides
SO2095: SO2095 - hypothetical protein (NCBI ptt file), at 2,192,061 to 2,193,914
SO2095
SO2096: SO2096 - hydrogenase expression/formation protein (NCBI ptt file), at 2,193,880 to 2,194,458
SO2096
SO2097: hydC - quinone-reactive Ni/Fe hydrogenase, cytochrome b subunit (NCBI ptt file), at 2,194,466 to 2,195,140
hydC
SO2098: hyaB - quinone-reactive Ni/Fe hydrogenase, large subunit (NCBI ptt file), at 2,195,162 to 2,196,865
hyaB
Position (kb)
2194
2195
2196 Strain fitness (log2 ratio)
-1
0
1 at 2193.511 kb on - strand, within SO2095 at 2193.736 kb on + strand at 2193.744 kb on - strand at 2193.863 kb on + strand at 2193.873 kb on + strand at 2193.930 kb on + strand at 2193.962 kb on - strand, within SO2096 at 2193.964 kb on + strand, within SO2096 at 2194.009 kb on + strand, within SO2096 at 2194.033 kb on + strand, within SO2096 at 2194.034 kb on - strand, within SO2096 at 2194.086 kb on + strand, within SO2096 at 2194.086 kb on + strand, within SO2096 at 2194.111 kb on + strand, within SO2096 at 2194.187 kb on - strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.355 kb on - strand, within SO2096 at 2194.540 kb on + strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.741 kb on + strand, within hydC at 2194.749 kb on - strand, within hydC at 2194.749 kb on - strand, within hydC at 2195.061 kb on - strand, within hydC at 2195.068 kb on + strand, within hydC at 2195.224 kb on + strand at 2195.280 kb on - strand at 2195.323 kb on + strand at 2195.468 kb on - strand, within hyaB at 2195.587 kb on - strand, within hyaB at 2195.613 kb on - strand, within hyaB at 2195.614 kb on - strand, within hyaB at 2195.641 kb on - strand, within hyaB at 2195.792 kb on + strand, within hyaB at 2195.800 kb on - strand, within hyaB at 2195.840 kb on + strand, within hyaB at 2195.883 kb on + strand, within hyaB at 2195.891 kb on - strand, within hyaB at 2195.913 kb on - strand, within hyaB at 2196.063 kb on + strand, within hyaB at 2196.112 kb on + strand, within hyaB
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 2,193,511 - SO2095 0.78 +0.7 2,193,736 + +0.1 2,193,744 - -0.7 2,193,863 + -0.2 2,193,873 + +1.0 2,193,930 + +0.5 2,193,962 - SO2096 0.14 -0.1 2,193,964 + SO2096 0.15 +0.2 2,194,009 + SO2096 0.22 +0.3 2,194,033 + SO2096 0.26 -0.1 2,194,034 - SO2096 0.27 +0.3 2,194,086 + SO2096 0.36 -0.5 2,194,086 + SO2096 0.36 -0.2 2,194,111 + SO2096 0.40 +0.3 2,194,187 - SO2096 0.53 -0.3 2,194,347 + SO2096 0.81 +0.9 2,194,347 + SO2096 0.81 -0.6 2,194,347 + SO2096 0.81 -0.0 2,194,355 - SO2096 0.82 +0.5 2,194,540 + hydC SO2097 0.11 +0.1 2,194,657 - hydC SO2097 0.28 +0.1 2,194,657 - hydC SO2097 0.28 +0.3 2,194,679 + hydC SO2097 0.32 -1.2 2,194,679 + hydC SO2097 0.32 -0.1 2,194,741 + hydC SO2097 0.41 +1.1 2,194,749 - hydC SO2097 0.42 -0.6 2,194,749 - hydC SO2097 0.42 -0.6 2,195,061 - hydC SO2097 0.88 -1.0 2,195,068 + hydC SO2097 0.89 +0.1 2,195,224 + +0.2 2,195,280 - -0.7 2,195,323 + +0.7 2,195,468 - hyaB SO2098 0.18 +0.7 2,195,587 - hyaB SO2098 0.25 -0.1 2,195,613 - hyaB SO2098 0.26 +0.2 2,195,614 - hyaB SO2098 0.27 +0.0 2,195,641 - hyaB SO2098 0.28 -0.2 2,195,792 + hyaB SO2098 0.37 +0.7 2,195,800 - hyaB SO2098 0.37 +0.3 2,195,840 + hyaB SO2098 0.40 +0.3 2,195,883 + hyaB SO2098 0.42 -0.8 2,195,891 - hyaB SO2098 0.43 +0.3 2,195,913 - hyaB SO2098 0.44 +0.3 2,196,063 + hyaB SO2098 0.53 -1.5 2,196,112 + hyaB SO2098 0.56 -0.1
Or see this region's nucleotide sequence