Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4062 and HP15_4063 are separated by 4 nucleotides HP15_4063 and HP15_4064 overlap by 4 nucleotides HP15_4064 and HP15_4065 overlap by 4 nucleotides
HP15_4062: HP15_4062 - spermidine/putrescine ABC transporter, ATP-binding protein, at 4,296,679 to 4,297,800
_4062
HP15_4063: HP15_4063 - spermidine/putrescine ABC transporter, permease protein PotB, at 4,297,805 to 4,298,650
_4063
HP15_4064: HP15_4064 - spermidine/putrescine ABC transporter membrane protein, at 4,298,647 to 4,299,420
_4064
HP15_4065: HP15_4065 - spermidine/putrescine ABC transporter periplasmic substrate-binding protein, at 4,299,417 to 4,300,457
_4065
Position (kb)
4298
4299
4300 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4297.656 kb on - strand, within HP15_4062 at 4297.705 kb on - strand at 4297.800 kb on + strand at 4297.822 kb on - strand at 4297.824 kb on + strand at 4297.840 kb on + strand at 4297.909 kb on - strand, within HP15_4063 at 4298.063 kb on + strand, within HP15_4063 at 4298.104 kb on - strand, within HP15_4063 at 4298.136 kb on - strand, within HP15_4063 at 4298.195 kb on + strand, within HP15_4063 at 4298.236 kb on + strand, within HP15_4063 at 4298.244 kb on - strand, within HP15_4063 at 4298.255 kb on + strand, within HP15_4063 at 4298.357 kb on - strand, within HP15_4063 at 4298.391 kb on + strand, within HP15_4063 at 4298.417 kb on + strand, within HP15_4063 at 4298.417 kb on + strand, within HP15_4063 at 4298.487 kb on + strand, within HP15_4063 at 4298.633 kb on + strand at 4298.646 kb on + strand at 4298.675 kb on + strand at 4298.683 kb on - strand at 4298.683 kb on - strand at 4298.685 kb on + strand at 4298.685 kb on + strand at 4298.760 kb on - strand, within HP15_4064 at 4298.761 kb on - strand, within HP15_4064 at 4298.791 kb on - strand, within HP15_4064 at 4298.858 kb on - strand, within HP15_4064 at 4298.902 kb on + strand, within HP15_4064 at 4299.029 kb on + strand, within HP15_4064 at 4299.032 kb on - strand, within HP15_4064 at 4299.093 kb on - strand, within HP15_4064 at 4299.162 kb on + strand, within HP15_4064 at 4299.221 kb on + strand, within HP15_4064 at 4299.221 kb on + strand, within HP15_4064 at 4299.221 kb on + strand, within HP15_4064 at 4299.221 kb on + strand, within HP15_4064 at 4299.229 kb on - strand, within HP15_4064 at 4299.229 kb on - strand, within HP15_4064 at 4299.229 kb on - strand, within HP15_4064 at 4299.229 kb on - strand, within HP15_4064 at 4299.229 kb on - strand, within HP15_4064 at 4299.231 kb on + strand, within HP15_4064 at 4299.302 kb on + strand, within HP15_4064 at 4299.310 kb on - strand, within HP15_4064 at 4299.372 kb on + strand at 4299.454 kb on + strand at 4299.462 kb on + strand at 4299.472 kb on - strand at 4299.477 kb on - strand at 4299.505 kb on + strand at 4299.513 kb on - strand at 4299.536 kb on + strand, within HP15_4065 at 4299.564 kb on - strand, within HP15_4065 at 4299.574 kb on - strand, within HP15_4065 at 4299.584 kb on - strand, within HP15_4065 at 4299.605 kb on - strand, within HP15_4065 at 4299.633 kb on + strand, within HP15_4065 at 4299.633 kb on + strand, within HP15_4065 at 4299.633 kb on + strand, within HP15_4065 at 4299.633 kb on + strand, within HP15_4065 at 4299.641 kb on + strand, within HP15_4065 at 4299.641 kb on - strand, within HP15_4065 at 4299.641 kb on - strand, within HP15_4065 at 4299.803 kb on + strand, within HP15_4065 at 4299.859 kb on + strand, within HP15_4065 at 4299.947 kb on + strand, within HP15_4065 at 4299.949 kb on - strand, within HP15_4065 at 4300.076 kb on - strand, within HP15_4065 at 4300.237 kb on - strand, within HP15_4065 at 4300.291 kb on + strand, within HP15_4065 at 4300.370 kb on + strand at 4300.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 15 mM remove 4,297,656 - HP15_4062 0.87 -2.8 4,297,705 - -2.2 4,297,800 + -3.1 4,297,822 - -3.2 4,297,824 + -1.1 4,297,840 + -0.5 4,297,909 - HP15_4063 0.12 -1.6 4,298,063 + HP15_4063 0.30 -2.1 4,298,104 - HP15_4063 0.35 -3.0 4,298,136 - HP15_4063 0.39 -3.6 4,298,195 + HP15_4063 0.46 -3.9 4,298,236 + HP15_4063 0.51 -2.1 4,298,244 - HP15_4063 0.52 -1.1 4,298,255 + HP15_4063 0.53 -0.9 4,298,357 - HP15_4063 0.65 -2.1 4,298,391 + HP15_4063 0.69 +0.8 4,298,417 + HP15_4063 0.72 -0.4 4,298,417 + HP15_4063 0.72 -0.9 4,298,487 + HP15_4063 0.81 -2.6 4,298,633 + -3.0 4,298,646 + +1.8 4,298,675 + -4.0 4,298,683 - -0.9 4,298,683 - +0.1 4,298,685 + -3.5 4,298,685 + +0.6 4,298,760 - HP15_4064 0.15 -4.0 4,298,761 - HP15_4064 0.15 -2.6 4,298,791 - HP15_4064 0.19 -1.8 4,298,858 - HP15_4064 0.27 -1.9 4,298,902 + HP15_4064 0.33 -2.9 4,299,029 + HP15_4064 0.49 +0.3 4,299,032 - HP15_4064 0.50 -0.6 4,299,093 - HP15_4064 0.58 -3.2 4,299,162 + HP15_4064 0.67 -3.8 4,299,221 + HP15_4064 0.74 -1.9 4,299,221 + HP15_4064 0.74 -2.9 4,299,221 + HP15_4064 0.74 -3.0 4,299,221 + HP15_4064 0.74 -3.5 4,299,229 - HP15_4064 0.75 +0.9 4,299,229 - HP15_4064 0.75 -1.9 4,299,229 - HP15_4064 0.75 -1.2 4,299,229 - HP15_4064 0.75 +1.9 4,299,229 - HP15_4064 0.75 -3.5 4,299,231 + HP15_4064 0.75 -2.0 4,299,302 + HP15_4064 0.85 -1.4 4,299,310 - HP15_4064 0.86 -1.0 4,299,372 + -1.9 4,299,454 + -1.9 4,299,462 + -3.9 4,299,472 - -1.7 4,299,477 - -3.5 4,299,505 + -2.2 4,299,513 - -2.8 4,299,536 + HP15_4065 0.11 -2.4 4,299,564 - HP15_4065 0.14 -1.8 4,299,574 - HP15_4065 0.15 -2.5 4,299,584 - HP15_4065 0.16 -3.1 4,299,605 - HP15_4065 0.18 -3.4 4,299,633 + HP15_4065 0.21 +0.4 4,299,633 + HP15_4065 0.21 -2.1 4,299,633 + HP15_4065 0.21 +1.6 4,299,633 + HP15_4065 0.21 -0.7 4,299,641 + HP15_4065 0.22 +0.1 4,299,641 - HP15_4065 0.22 +1.4 4,299,641 - HP15_4065 0.22 -2.6 4,299,803 + HP15_4065 0.37 -1.4 4,299,859 + HP15_4065 0.42 -3.2 4,299,947 + HP15_4065 0.51 -1.0 4,299,949 - HP15_4065 0.51 -3.5 4,300,076 - HP15_4065 0.63 +1.6 4,300,237 - HP15_4065 0.79 -4.8 4,300,291 + HP15_4065 0.84 +0.9 4,300,370 + -2.8 4,300,373 - -1.8
Or see this region's nucleotide sequence