Experiment: m.b. 1-ethyl-3-methylimidazolium acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_300 and HP15_301 are separated by 168 nucleotides HP15_301 and HP15_302 are separated by 21 nucleotides HP15_302 and HP15_303 are separated by 148 nucleotides
HP15_300: HP15_300 - ribonuclease PH, at 291,114 to 291,830
_300
HP15_301: HP15_301 - conserved hypothetical protein, at 291,999 to 292,865
_301
HP15_302: HP15_302 - protein containing pseudouridine synthase domain, at 292,887 to 293,708
_302
HP15_303: HP15_303 - guanylate kinase, at 293,857 to 294,489
_303
Position (kb) 291
292
293 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 291.018 kb on - strand at 291.033 kb on - strand at 291.128 kb on - strand at 291.152 kb on + strand at 291.160 kb on - strand at 291.194 kb on - strand, within HP15_300 at 291.225 kb on + strand, within HP15_300 at 291.293 kb on + strand, within HP15_300 at 291.303 kb on + strand, within HP15_300 at 291.311 kb on - strand, within HP15_300 at 291.311 kb on - strand, within HP15_300 at 291.654 kb on + strand, within HP15_300 at 291.655 kb on + strand, within HP15_300 at 291.668 kb on - strand, within HP15_300 at 291.746 kb on + strand, within HP15_300 at 291.746 kb on + strand, within HP15_300 at 291.793 kb on + strand at 291.801 kb on - strand at 291.811 kb on - strand at 291.868 kb on + strand at 291.918 kb on - strand at 291.955 kb on - strand at 291.955 kb on - strand at 291.955 kb on - strand at 291.991 kb on + strand at 291.999 kb on - strand at 292.034 kb on + strand at 292.042 kb on - strand at 292.053 kb on + strand at 292.056 kb on - strand at 292.061 kb on - strand at 292.061 kb on - strand at 292.075 kb on - strand at 292.182 kb on + strand, within HP15_301 at 292.213 kb on + strand, within HP15_301 at 292.310 kb on + strand, within HP15_301 at 292.325 kb on - strand, within HP15_301 at 292.373 kb on - strand, within HP15_301 at 292.373 kb on - strand, within HP15_301 at 292.373 kb on - strand, within HP15_301 at 292.375 kb on + strand, within HP15_301 at 292.511 kb on + strand, within HP15_301 at 292.532 kb on - strand, within HP15_301 at 292.605 kb on + strand, within HP15_301 at 292.613 kb on - strand, within HP15_301 at 292.613 kb on - strand, within HP15_301 at 292.807 kb on - strand at 292.903 kb on - strand at 292.939 kb on + strand at 292.944 kb on + strand at 292.944 kb on + strand at 292.944 kb on + strand at 292.952 kb on - strand at 292.983 kb on + strand, within HP15_302 at 293.116 kb on + strand, within HP15_302 at 293.134 kb on - strand, within HP15_302 at 293.233 kb on + strand, within HP15_302 at 293.266 kb on - strand, within HP15_302 at 293.317 kb on + strand, within HP15_302 at 293.425 kb on + strand, within HP15_302 at 293.440 kb on + strand, within HP15_302 at 293.440 kb on + strand, within HP15_302 at 293.448 kb on - strand, within HP15_302 at 293.448 kb on - strand, within HP15_302 at 293.553 kb on + strand, within HP15_302 at 293.664 kb on + strand at 293.678 kb on + strand at 293.734 kb on - strand at 293.745 kb on - strand at 293.759 kb on + strand at 293.759 kb on + strand at 293.759 kb on + strand at 293.760 kb on - strand at 293.767 kb on - strand at 293.769 kb on - strand at 293.769 kb on - strand at 293.838 kb on + strand at 293.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium acetate 15 mM remove 291,018 - -2.2 291,033 - +0.7 291,128 - -0.5 291,152 + +2.0 291,160 - +1.5 291,194 - HP15_300 0.11 +1.7 291,225 + HP15_300 0.15 +1.8 291,293 + HP15_300 0.25 +2.2 291,303 + HP15_300 0.26 -0.1 291,311 - HP15_300 0.27 +1.7 291,311 - HP15_300 0.27 -1.0 291,654 + HP15_300 0.75 +1.0 291,655 + HP15_300 0.75 +2.1 291,668 - HP15_300 0.77 +2.2 291,746 + HP15_300 0.88 +1.1 291,746 + HP15_300 0.88 +0.8 291,793 + +1.2 291,801 - +1.4 291,811 - +0.7 291,868 + +1.8 291,918 - -0.9 291,955 - +1.0 291,955 - -0.2 291,955 - +1.1 291,991 + +0.0 291,999 - +0.9 292,034 + -0.3 292,042 - -1.1 292,053 + -0.7 292,056 - +0.3 292,061 - -2.3 292,061 - -2.0 292,075 - -0.5 292,182 + HP15_301 0.21 +0.5 292,213 + HP15_301 0.25 -2.0 292,310 + HP15_301 0.36 +0.1 292,325 - HP15_301 0.38 +1.0 292,373 - HP15_301 0.43 +0.2 292,373 - HP15_301 0.43 +0.4 292,373 - HP15_301 0.43 +1.1 292,375 + HP15_301 0.43 +0.3 292,511 + HP15_301 0.59 +1.2 292,532 - HP15_301 0.61 +1.3 292,605 + HP15_301 0.70 -2.6 292,613 - HP15_301 0.71 -0.0 292,613 - HP15_301 0.71 -0.9 292,807 - -1.2 292,903 - -3.4 292,939 + +2.1 292,944 + -0.5 292,944 + -0.3 292,944 + +0.4 292,952 - -2.0 292,983 + HP15_302 0.12 -1.1 293,116 + HP15_302 0.28 -0.7 293,134 - HP15_302 0.30 +0.7 293,233 + HP15_302 0.42 -0.3 293,266 - HP15_302 0.46 +0.8 293,317 + HP15_302 0.52 +0.5 293,425 + HP15_302 0.65 +0.3 293,440 + HP15_302 0.67 -0.6 293,440 + HP15_302 0.67 +0.5 293,448 - HP15_302 0.68 +0.4 293,448 - HP15_302 0.68 +1.7 293,553 + HP15_302 0.81 +0.7 293,664 + -0.1 293,678 + +1.3 293,734 - +1.0 293,745 - -1.3 293,759 + +0.1 293,759 + +0.7 293,759 + -0.4 293,760 - -0.6 293,767 - -0.1 293,769 - -1.0 293,769 - +0.3 293,838 + +0.6 293,838 + -0.3
Or see this region's nucleotide sequence