Experiment: Gly-Glu (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuD and leuC are separated by 24 nucleotides leuC and leuB overlap by 1 nucleotides
SO4233: leuD - 3-isopropylmalate dehydratase, small subunit (NCBI ptt file), at 4,410,417 to 4,411,022
leuD
SO4234: leuC - 3-isopropylmalate dehydratase, large subunit (NCBI ptt file), at 4,411,047 to 4,412,471
leuC
SO4235: leuB - 3-isopropylmalate dehydrogenase (NCBI ptt file), at 4,412,471 to 4,413,565
leuB
Position (kb)
4411
4412
4413 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4410.477 kb on + strand at 4410.477 kb on + strand at 4410.482 kb on + strand, within leuD at 4410.485 kb on - strand, within leuD at 4410.485 kb on - strand, within leuD at 4410.485 kb on - strand, within leuD at 4410.491 kb on + strand, within leuD at 4410.491 kb on + strand, within leuD at 4410.681 kb on - strand, within leuD at 4410.834 kb on + strand, within leuD at 4410.837 kb on - strand, within leuD at 4410.837 kb on - strand, within leuD at 4410.951 kb on + strand, within leuD at 4411.054 kb on + strand at 4411.062 kb on - strand at 4411.113 kb on + strand at 4411.113 kb on + strand at 4411.121 kb on - strand at 4411.121 kb on - strand at 4411.130 kb on + strand at 4411.147 kb on - strand at 4411.220 kb on + strand, within leuC at 4411.220 kb on + strand, within leuC at 4411.320 kb on - strand, within leuC at 4411.349 kb on + strand, within leuC at 4411.388 kb on - strand, within leuC at 4411.471 kb on + strand, within leuC at 4411.621 kb on - strand, within leuC at 4411.624 kb on - strand, within leuC at 4411.626 kb on + strand, within leuC at 4411.651 kb on - strand, within leuC at 4411.680 kb on + strand, within leuC at 4411.715 kb on + strand, within leuC at 4411.768 kb on - strand, within leuC at 4411.815 kb on - strand, within leuC at 4411.928 kb on - strand, within leuC at 4412.105 kb on + strand, within leuC at 4412.113 kb on - strand, within leuC at 4412.221 kb on - strand, within leuC at 4412.336 kb on - strand at 4412.358 kb on + strand at 4412.484 kb on - strand at 4412.555 kb on - strand at 4412.608 kb on - strand, within leuB at 4412.629 kb on + strand, within leuB at 4412.629 kb on + strand, within leuB at 4412.637 kb on - strand, within leuB at 4412.784 kb on + strand, within leuB at 4412.830 kb on + strand, within leuB at 4412.838 kb on - strand, within leuB at 4412.838 kb on - strand, within leuB at 4412.847 kb on + strand, within leuB at 4412.848 kb on + strand, within leuB at 4412.855 kb on - strand, within leuB at 4412.857 kb on + strand, within leuB at 4412.895 kb on + strand, within leuB at 4412.957 kb on + strand, within leuB at 4412.957 kb on + strand, within leuB at 4412.965 kb on - strand, within leuB at 4412.979 kb on + strand, within leuB at 4412.980 kb on - strand, within leuB at 4413.068 kb on + strand, within leuB at 4413.083 kb on + strand, within leuB at 4413.103 kb on + strand, within leuB at 4413.104 kb on + strand, within leuB at 4413.105 kb on + strand, within leuB at 4413.120 kb on + strand, within leuB at 4413.128 kb on - strand, within leuB at 4413.217 kb on + strand, within leuB at 4413.218 kb on + strand, within leuB at 4413.294 kb on - strand, within leuB at 4413.389 kb on - strand, within leuB at 4413.396 kb on - strand, within leuB at 4413.419 kb on + strand, within leuB
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (C) remove 4,410,477 + -3.4 4,410,477 + -4.6 4,410,482 + leuD SO4233 0.11 -1.1 4,410,485 - leuD SO4233 0.11 -1.1 4,410,485 - leuD SO4233 0.11 -3.8 4,410,485 - leuD SO4233 0.11 -4.0 4,410,491 + leuD SO4233 0.12 -2.0 4,410,491 + leuD SO4233 0.12 -2.2 4,410,681 - leuD SO4233 0.44 -2.9 4,410,834 + leuD SO4233 0.69 -3.0 4,410,837 - leuD SO4233 0.69 -4.2 4,410,837 - leuD SO4233 0.69 -2.4 4,410,951 + leuD SO4233 0.88 -3.3 4,411,054 + -1.9 4,411,062 - -2.4 4,411,113 + -3.4 4,411,113 + -3.3 4,411,121 - -2.0 4,411,121 - -2.5 4,411,130 + +0.2 4,411,147 - -3.6 4,411,220 + leuC SO4234 0.12 -2.5 4,411,220 + leuC SO4234 0.12 -2.4 4,411,320 - leuC SO4234 0.19 -3.6 4,411,349 + leuC SO4234 0.21 -2.7 4,411,388 - leuC SO4234 0.24 -2.1 4,411,471 + leuC SO4234 0.30 -3.9 4,411,621 - leuC SO4234 0.40 -0.8 4,411,624 - leuC SO4234 0.40 -3.2 4,411,626 + leuC SO4234 0.41 -3.9 4,411,651 - leuC SO4234 0.42 -3.6 4,411,680 + leuC SO4234 0.44 -4.7 4,411,715 + leuC SO4234 0.47 -2.9 4,411,768 - leuC SO4234 0.51 -2.1 4,411,815 - leuC SO4234 0.54 -4.0 4,411,928 - leuC SO4234 0.62 -2.7 4,412,105 + leuC SO4234 0.74 -3.5 4,412,113 - leuC SO4234 0.75 -4.2 4,412,221 - leuC SO4234 0.82 -3.0 4,412,336 - -3.4 4,412,358 + -2.4 4,412,484 - -1.2 4,412,555 - -1.6 4,412,608 - leuB SO4235 0.13 -2.9 4,412,629 + leuB SO4235 0.14 -4.7 4,412,629 + leuB SO4235 0.14 -3.0 4,412,637 - leuB SO4235 0.15 -2.6 4,412,784 + leuB SO4235 0.29 -1.4 4,412,830 + leuB SO4235 0.33 -2.2 4,412,838 - leuB SO4235 0.34 -3.2 4,412,838 - leuB SO4235 0.34 -3.7 4,412,847 + leuB SO4235 0.34 -1.1 4,412,848 + leuB SO4235 0.34 -4.1 4,412,855 - leuB SO4235 0.35 -3.7 4,412,857 + leuB SO4235 0.35 -1.6 4,412,895 + leuB SO4235 0.39 -3.6 4,412,957 + leuB SO4235 0.44 -3.0 4,412,957 + leuB SO4235 0.44 -2.6 4,412,965 - leuB SO4235 0.45 -4.7 4,412,979 + leuB SO4235 0.46 -4.3 4,412,980 - leuB SO4235 0.46 -2.4 4,413,068 + leuB SO4235 0.55 -5.0 4,413,083 + leuB SO4235 0.56 -2.1 4,413,103 + leuB SO4235 0.58 -3.5 4,413,104 + leuB SO4235 0.58 -3.6 4,413,105 + leuB SO4235 0.58 -4.1 4,413,120 + leuB SO4235 0.59 -3.3 4,413,128 - leuB SO4235 0.60 -3.5 4,413,217 + leuB SO4235 0.68 +0.2 4,413,218 + leuB SO4235 0.68 -4.4 4,413,294 - leuB SO4235 0.75 -4.2 4,413,389 - leuB SO4235 0.84 -4.1 4,413,396 - leuB SO4235 0.84 -3.0 4,413,419 + leuB SO4235 0.87 -0.4
Or see this region's nucleotide sequence