Experiment: Gly-Glu (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2333 and seqA are separated by 230 nucleotides seqA and pgm are separated by 70 nucleotides pgm and msrA are separated by 110 nucleotides msrA and astE are separated by 278 nucleotides
SO2333: SO2333 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,440,959 to 2,441,801
SO2333
SO2335: seqA - seqA protein (NCBI ptt file), at 2,442,032 to 2,442,583
seqA
SO2336: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (NCBI ptt file), at 2,442,654 to 2,444,306
pgm
SO2337: msrA - peptide methionine sulfoxide reductase (NCBI ptt file), at 2,444,417 to 2,444,896
msrA
SO2338: astE - succinylglutamate desuccinylase (NCBI ptt file), at 2,445,175 to 2,446,209
astE
Position (kb)
2442
2443
2444
2445 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 2441.756 kb on + strand at 2441.756 kb on + strand at 2441.764 kb on - strand at 2441.810 kb on - strand at 2441.945 kb on + strand at 2441.950 kb on + strand at 2442.767 kb on - strand at 2443.527 kb on - strand, within pgm at 2443.841 kb on - strand, within pgm at 2444.254 kb on - strand at 2444.403 kb on - strand at 2444.883 kb on + strand at 2444.991 kb on + strand at 2445.030 kb on + strand at 2445.074 kb on + strand at 2445.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (C) remove 2,441,756 + +1.1 2,441,756 + +0.4 2,441,764 - -1.0 2,441,810 - -0.2 2,441,945 + +0.3 2,441,950 + +0.1 2,442,767 - +6.6 2,443,527 - pgm SO2336 0.53 +6.8 2,443,841 - pgm SO2336 0.72 +6.8 2,444,254 - +6.4 2,444,403 - +0.1 2,444,883 + +0.6 2,444,991 + -0.8 2,445,030 + +0.3 2,445,074 + +0.2 2,445,116 - -1.0
Or see this region's nucleotide sequence