Strain Fitness in Shewanella oneidensis MR-1 around SO2336

Experiment: Gly-Glu (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2333 and seqA are separated by 230 nucleotidesseqA and pgm are separated by 70 nucleotidespgm and msrA are separated by 110 nucleotidesmsrA and astE are separated by 278 nucleotides SO2333: SO2333 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,440,959 to 2,441,801 SO2333 SO2335: seqA - seqA protein (NCBI ptt file), at 2,442,032 to 2,442,583 seqA SO2336: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (NCBI ptt file), at 2,442,654 to 2,444,306 pgm SO2337: msrA - peptide methionine sulfoxide reductase (NCBI ptt file), at 2,444,417 to 2,444,896 msrA SO2338: astE - succinylglutamate desuccinylase (NCBI ptt file), at 2,445,175 to 2,446,209 astE Position (kb) 2442 2443 2444 2445Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 2441.756 kb on + strandat 2441.756 kb on + strandat 2441.764 kb on - strandat 2441.810 kb on - strandat 2441.945 kb on + strandat 2441.950 kb on + strandat 2442.767 kb on - strandat 2443.527 kb on - strand, within pgmat 2443.841 kb on - strand, within pgmat 2444.254 kb on - strandat 2444.403 kb on - strandat 2444.883 kb on + strandat 2444.991 kb on + strandat 2445.030 kb on + strandat 2445.074 kb on + strandat 2445.116 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-Glu (C)
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2,441,756 + +1.1
2,441,756 + +0.4
2,441,764 - -1.0
2,441,810 - -0.2
2,441,945 + +0.3
2,441,950 + +0.1
2,442,767 - +6.6
2,443,527 - pgm SO2336 0.53 +6.8
2,443,841 - pgm SO2336 0.72 +6.8
2,444,254 - +6.4
2,444,403 - +0.1
2,444,883 + +0.6
2,444,991 + -0.8
2,445,030 + +0.3
2,445,074 + +0.2
2,445,116 - -1.0

Or see this region's nucleotide sequence