Experiment: marine broth pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20530 and PGA1_c20535 are separated by 66 nucleotides PGA1_c20535 and PGA1_c20540 are separated by 219 nucleotides PGA1_c20540 and PGA1_c20550 are separated by 720 nucleotides
PGA1_c20530: PGA1_c20530 - putative transcriptional regulator, at 2,127,119 to 2,127,541
_c20530
PGA1_c20535: PGA1_c20535 - hypothetical protein, at 2,127,608 to 2,128,015
_c20535
PGA1_c20540: PGA1_c20540 - ATP-dependent protease La, at 2,128,235 to 2,130,649
_c20540
PGA1_c20550: PGA1_c20550 - hypothetical protein, at 2,131,370 to 2,131,831
_c20550
Position (kb)
2128
2129
2130
2131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2127.303 kb on - strand, within PGA1_c20530 at 2127.315 kb on + strand, within PGA1_c20530 at 2127.396 kb on + strand, within PGA1_c20530 at 2127.446 kb on - strand, within PGA1_c20530 at 2127.469 kb on + strand, within PGA1_c20530 at 2127.477 kb on - strand, within PGA1_c20530 at 2127.495 kb on + strand, within PGA1_c20530 at 2127.513 kb on + strand at 2127.513 kb on + strand at 2127.521 kb on - strand at 2127.521 kb on - strand at 2127.521 kb on - strand at 2127.618 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on - strand at 2127.628 kb on + strand at 2127.631 kb on + strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.683 kb on - strand, within PGA1_c20535 at 2127.779 kb on + strand, within PGA1_c20535 at 2127.835 kb on - strand, within PGA1_c20535 at 2127.837 kb on - strand, within PGA1_c20535 at 2127.844 kb on - strand, within PGA1_c20535 at 2127.844 kb on - strand, within PGA1_c20535 at 2127.911 kb on - strand, within PGA1_c20535 at 2127.927 kb on + strand, within PGA1_c20535 at 2127.927 kb on + strand, within PGA1_c20535 at 2128.022 kb on - strand at 2128.116 kb on + strand at 2128.116 kb on + strand at 2130.762 kb on + strand at 2130.762 kb on + strand at 2130.762 kb on + strand at 2130.801 kb on + strand at 2130.809 kb on - strand at 2130.811 kb on - strand at 2130.811 kb on - strand at 2130.816 kb on + strand at 2130.891 kb on + strand at 2130.914 kb on - strand at 2130.919 kb on - strand at 2130.924 kb on - strand at 2130.924 kb on - strand at 2130.924 kb on - strand at 2130.934 kb on - strand at 2130.947 kb on - strand at 2130.977 kb on - strand at 2130.977 kb on - strand at 2130.977 kb on - strand at 2130.977 kb on - strand at 2130.977 kb on - strand at 2130.977 kb on - strand at 2131.069 kb on + strand at 2131.074 kb on + strand at 2131.082 kb on - strand at 2131.084 kb on + strand at 2131.105 kb on - strand at 2131.116 kb on + strand at 2131.116 kb on + strand at 2131.134 kb on + strand at 2131.139 kb on + strand at 2131.170 kb on - strand at 2131.209 kb on + strand at 2131.209 kb on + strand at 2131.209 kb on + strand at 2131.217 kb on - strand at 2131.243 kb on - strand at 2131.368 kb on + strand at 2131.376 kb on - strand at 2131.376 kb on - strand at 2131.438 kb on - strand, within PGA1_c20550 at 2131.440 kb on + strand, within PGA1_c20550 at 2131.440 kb on + strand, within PGA1_c20550 at 2131.463 kb on - strand, within PGA1_c20550 at 2131.469 kb on + strand, within PGA1_c20550 at 2131.535 kb on + strand, within PGA1_c20550 at 2131.543 kb on - strand, within PGA1_c20550 at 2131.543 kb on - strand, within PGA1_c20550 at 2131.587 kb on + strand, within PGA1_c20550 at 2131.603 kb on + strand, within PGA1_c20550 at 2131.611 kb on - strand, within PGA1_c20550 at 2131.611 kb on - strand, within PGA1_c20550 at 2131.649 kb on + strand, within PGA1_c20550
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth pH9 remove 2,127,303 - PGA1_c20530 0.43 -0.2 2,127,315 + PGA1_c20530 0.46 +1.0 2,127,396 + PGA1_c20530 0.65 +0.1 2,127,446 - PGA1_c20530 0.77 -2.6 2,127,469 + PGA1_c20530 0.83 +0.2 2,127,477 - PGA1_c20530 0.85 -0.1 2,127,495 + PGA1_c20530 0.89 +1.1 2,127,513 + +1.5 2,127,513 + -1.0 2,127,521 - -0.6 2,127,521 - +0.8 2,127,521 - +1.0 2,127,618 + +0.6 2,127,626 + -0.3 2,127,626 + -1.0 2,127,626 + +0.7 2,127,626 + +0.9 2,127,626 + -2.5 2,127,626 + +0.6 2,127,626 + -2.3 2,127,626 + -0.8 2,127,626 + -0.0 2,127,626 - +0.8 2,127,628 + +0.9 2,127,631 + +0.2 2,127,634 - -2.5 2,127,634 - +0.5 2,127,634 - -2.7 2,127,634 - -0.3 2,127,634 - -0.6 2,127,634 - -1.6 2,127,634 - -0.3 2,127,683 - PGA1_c20535 0.18 -0.5 2,127,779 + PGA1_c20535 0.42 -0.4 2,127,835 - PGA1_c20535 0.56 -0.6 2,127,837 - PGA1_c20535 0.56 -0.5 2,127,844 - PGA1_c20535 0.58 -1.1 2,127,844 - PGA1_c20535 0.58 +1.0 2,127,911 - PGA1_c20535 0.74 -1.3 2,127,927 + PGA1_c20535 0.78 -0.3 2,127,927 + PGA1_c20535 0.78 -0.8 2,128,022 - +0.2 2,128,116 + -1.4 2,128,116 + +0.4 2,130,762 + +0.4 2,130,762 + -0.4 2,130,762 + +1.1 2,130,801 + +0.2 2,130,809 - -1.1 2,130,811 - -0.9 2,130,811 - +1.4 2,130,816 + -0.1 2,130,891 + -1.1 2,130,914 - -0.1 2,130,919 - -1.1 2,130,924 - -0.9 2,130,924 - -0.6 2,130,924 - -0.8 2,130,934 - +0.7 2,130,947 - -2.3 2,130,977 - +0.9 2,130,977 - -1.2 2,130,977 - -0.1 2,130,977 - +0.7 2,130,977 - +0.2 2,130,977 - -0.7 2,131,069 + -0.7 2,131,074 + -1.6 2,131,082 - -0.2 2,131,084 + +0.7 2,131,105 - -1.3 2,131,116 + +0.9 2,131,116 + +0.6 2,131,134 + +0.1 2,131,139 + -1.8 2,131,170 - +0.9 2,131,209 + -0.8 2,131,209 + -2.4 2,131,209 + +0.9 2,131,217 - +1.0 2,131,243 - -0.0 2,131,368 + -0.7 2,131,376 - -0.6 2,131,376 - -2.5 2,131,438 - PGA1_c20550 0.15 -0.9 2,131,440 + PGA1_c20550 0.15 +0.2 2,131,440 + PGA1_c20550 0.15 -0.4 2,131,463 - PGA1_c20550 0.20 +0.5 2,131,469 + PGA1_c20550 0.21 +0.1 2,131,535 + PGA1_c20550 0.36 +0.9 2,131,543 - PGA1_c20550 0.37 +0.2 2,131,543 - PGA1_c20550 0.37 -3.4 2,131,587 + PGA1_c20550 0.47 -0.4 2,131,603 + PGA1_c20550 0.50 +0.0 2,131,611 - PGA1_c20550 0.52 +0.2 2,131,611 - PGA1_c20550 0.52 -1.3 2,131,649 + PGA1_c20550 0.60 +1.9
Or see this region's nucleotide sequence