Experiment: Aluminum chloride 0.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrG and eno are separated by 218 nucleotides eno and Sama_1037 are separated by 181 nucleotides Sama_1037 and ispD overlap by 4 nucleotides
Sama_1035: pyrG - CTP synthetase (RefSeq), at 1,253,255 to 1,254,892
pyrG
Sama_1036: eno - phosphopyruvate hydratase (RefSeq), at 1,255,111 to 1,256,406
eno
Sama_1037: Sama_1037 - cell division protein FtsB (RefSeq), at 1,256,588 to 1,256,905
_1037
Sama_1038: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,256,902 to 1,257,618
ispD
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-1
0
1 at 1254.890 kb on + strand at 1254.890 kb on + strand at 1254.963 kb on + strand at 1254.964 kb on - strand at 1255.000 kb on + strand at 1255.042 kb on + strand at 1255.057 kb on + strand at 1255.085 kb on + strand at 1255.085 kb on + strand at 1256.415 kb on + strand at 1256.423 kb on - strand at 1256.432 kb on + strand at 1256.446 kb on + strand at 1256.447 kb on - strand at 1256.448 kb on + strand at 1256.448 kb on + strand at 1256.448 kb on + strand at 1256.448 kb on + strand at 1256.448 kb on + strand at 1256.449 kb on - strand at 1256.449 kb on - strand at 1256.449 kb on - strand at 1256.484 kb on + strand at 1256.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Aluminum chloride 0.625 mM remove 1,254,890 + +0.1 1,254,890 + +0.2 1,254,963 + +0.4 1,254,964 - +0.3 1,255,000 + +0.0 1,255,042 + +1.4 1,255,057 + -1.4 1,255,085 + -0.1 1,255,085 + -0.8 1,256,415 + -1.1 1,256,423 - +0.4 1,256,432 + -0.4 1,256,446 + -0.8 1,256,447 - +0.2 1,256,448 + +0.2 1,256,448 + +0.1 1,256,448 + +0.0 1,256,448 + -0.3 1,256,448 + +0.0 1,256,449 - +1.2 1,256,449 - +1.0 1,256,449 - +1.7 1,256,484 + -0.4 1,256,485 - +0.2
Or see this region's nucleotide sequence