Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29780 and PGA1_c29790 are separated by 12 nucleotides PGA1_c29790 and PGA1_c29800 are separated by 57 nucleotides PGA1_c29800 and PGA1_c29810 are separated by 371 nucleotides
PGA1_c29780: PGA1_c29780 - 3-isopropylmalate dehydratase large subunit, at 3,114,596 to 3,115,999
_c29780
PGA1_c29790: PGA1_c29790 - 3-isopropylmalate dehydratase small subunit, at 3,116,012 to 3,116,617
_c29790
PGA1_c29800: PGA1_c29800 - Uncharacterized protein conserved in bacteria, at 3,116,675 to 3,117,664
_c29800
PGA1_c29810: PGA1_c29810 - hypothetical protein, at 3,118,036 to 3,118,869
_c29810
Position (kb)
3116
3117
3118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3115.744 kb on - strand, within PGA1_c29780 at 3115.744 kb on - strand, within PGA1_c29780 at 3115.780 kb on + strand, within PGA1_c29780 at 3115.852 kb on + strand, within PGA1_c29780 at 3115.891 kb on - strand at 3115.916 kb on + strand at 3115.923 kb on - strand at 3115.945 kb on + strand at 3115.993 kb on + strand at 3115.993 kb on + strand at 3115.993 kb on + strand at 3115.993 kb on + strand at 3115.993 kb on + strand at 3115.995 kb on + strand at 3116.001 kb on - strand at 3116.001 kb on - strand at 3116.001 kb on - strand at 3116.067 kb on - strand at 3116.085 kb on + strand, within PGA1_c29790 at 3116.306 kb on - strand, within PGA1_c29790 at 3116.474 kb on - strand, within PGA1_c29790 at 3116.543 kb on + strand, within PGA1_c29790 at 3116.543 kb on + strand, within PGA1_c29790 at 3116.551 kb on - strand, within PGA1_c29790 at 3116.592 kb on - strand at 3116.619 kb on - strand at 3116.633 kb on + strand at 3116.659 kb on + strand at 3116.756 kb on - strand at 3116.774 kb on - strand, within PGA1_c29800 at 3116.791 kb on + strand, within PGA1_c29800 at 3116.791 kb on + strand, within PGA1_c29800 at 3116.791 kb on + strand, within PGA1_c29800 at 3116.799 kb on - strand, within PGA1_c29800 at 3116.799 kb on - strand, within PGA1_c29800 at 3116.799 kb on - strand, within PGA1_c29800 at 3116.799 kb on - strand, within PGA1_c29800 at 3116.830 kb on + strand, within PGA1_c29800 at 3116.871 kb on - strand, within PGA1_c29800 at 3116.891 kb on + strand, within PGA1_c29800 at 3116.899 kb on + strand, within PGA1_c29800 at 3116.909 kb on + strand, within PGA1_c29800 at 3116.909 kb on + strand, within PGA1_c29800 at 3116.909 kb on + strand, within PGA1_c29800 at 3116.909 kb on + strand, within PGA1_c29800 at 3116.909 kb on - strand, within PGA1_c29800 at 3116.917 kb on - strand, within PGA1_c29800 at 3116.917 kb on - strand, within PGA1_c29800 at 3116.966 kb on - strand, within PGA1_c29800 at 3116.971 kb on - strand, within PGA1_c29800 at 3116.981 kb on - strand, within PGA1_c29800 at 3116.989 kb on + strand, within PGA1_c29800 at 3117.012 kb on + strand, within PGA1_c29800 at 3117.012 kb on + strand, within PGA1_c29800 at 3117.018 kb on + strand, within PGA1_c29800 at 3117.020 kb on - strand, within PGA1_c29800 at 3117.020 kb on - strand, within PGA1_c29800 at 3117.059 kb on + strand, within PGA1_c29800 at 3117.067 kb on + strand, within PGA1_c29800 at 3117.075 kb on - strand, within PGA1_c29800 at 3117.080 kb on - strand, within PGA1_c29800 at 3117.085 kb on + strand, within PGA1_c29800 at 3117.118 kb on + strand, within PGA1_c29800 at 3117.118 kb on + strand, within PGA1_c29800 at 3117.118 kb on + strand, within PGA1_c29800 at 3117.124 kb on - strand, within PGA1_c29800 at 3117.128 kb on + strand, within PGA1_c29800 at 3117.128 kb on + strand, within PGA1_c29800 at 3117.136 kb on - strand, within PGA1_c29800 at 3117.136 kb on - strand, within PGA1_c29800 at 3117.183 kb on - strand, within PGA1_c29800 at 3117.203 kb on - strand, within PGA1_c29800 at 3117.247 kb on + strand, within PGA1_c29800 at 3117.247 kb on + strand, within PGA1_c29800 at 3117.255 kb on - strand, within PGA1_c29800 at 3117.255 kb on - strand, within PGA1_c29800 at 3117.271 kb on - strand, within PGA1_c29800 at 3117.356 kb on + strand, within PGA1_c29800 at 3117.449 kb on + strand, within PGA1_c29800 at 3117.473 kb on - strand, within PGA1_c29800 at 3117.580 kb on + strand at 3117.588 kb on - strand at 3117.615 kb on + strand at 3117.615 kb on + strand at 3117.615 kb on + strand at 3117.615 kb on + strand at 3117.615 kb on + strand at 3117.623 kb on - strand at 3117.623 kb on - strand at 3117.623 kb on - strand at 3117.623 kb on - strand at 3117.659 kb on + strand at 3117.696 kb on + strand at 3117.696 kb on + strand at 3117.701 kb on + strand at 3117.718 kb on + strand at 3117.944 kb on - strand at 3117.980 kb on + strand at 3117.988 kb on - strand at 3118.003 kb on + strand at 3118.011 kb on - strand at 3118.047 kb on + strand at 3118.047 kb on + strand at 3118.047 kb on + strand at 3118.055 kb on - strand at 3118.150 kb on - strand, within PGA1_c29810 at 3118.171 kb on + strand, within PGA1_c29810 at 3118.171 kb on + strand, within PGA1_c29810 at 3118.171 kb on + strand, within PGA1_c29810 at 3118.171 kb on + strand, within PGA1_c29810 at 3118.171 kb on + strand, within PGA1_c29810 at 3118.234 kb on + strand, within PGA1_c29810 at 3118.234 kb on + strand, within PGA1_c29810 at 3118.234 kb on + strand, within PGA1_c29810 at 3118.242 kb on - strand, within PGA1_c29810 at 3118.264 kb on + strand, within PGA1_c29810 at 3118.318 kb on + strand, within PGA1_c29810 at 3118.331 kb on - strand, within PGA1_c29810 at 3118.332 kb on + strand, within PGA1_c29810 at 3118.339 kb on + strand, within PGA1_c29810 at 3118.354 kb on - strand, within PGA1_c29810 at 3118.449 kb on + strand, within PGA1_c29810 at 3118.466 kb on + strand, within PGA1_c29810 at 3118.467 kb on - strand, within PGA1_c29810 at 3118.467 kb on - strand, within PGA1_c29810 at 3118.467 kb on - strand, within PGA1_c29810 at 3118.524 kb on - strand, within PGA1_c29810 at 3118.564 kb on - strand, within PGA1_c29810 at 3118.655 kb on - strand, within PGA1_c29810 at 3118.661 kb on - strand, within PGA1_c29810
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 3,115,744 - PGA1_c29780 0.82 -2.3 3,115,744 - PGA1_c29780 0.82 -2.9 3,115,780 + PGA1_c29780 0.84 -3.3 3,115,852 + PGA1_c29780 0.89 -3.3 3,115,891 - -3.6 3,115,916 + -2.0 3,115,923 - -1.3 3,115,945 + -4.5 3,115,993 + -0.6 3,115,993 + +2.4 3,115,993 + -0.0 3,115,993 + +1.3 3,115,993 + -1.9 3,115,995 + -0.3 3,116,001 - -0.2 3,116,001 - +0.1 3,116,001 - -0.2 3,116,067 - -3.6 3,116,085 + PGA1_c29790 0.12 -3.4 3,116,306 - PGA1_c29790 0.49 -2.5 3,116,474 - PGA1_c29790 0.76 -3.5 3,116,543 + PGA1_c29790 0.88 -3.0 3,116,543 + PGA1_c29790 0.88 -3.6 3,116,551 - PGA1_c29790 0.89 -3.8 3,116,592 - -2.3 3,116,619 - -0.4 3,116,633 + +0.2 3,116,659 + -0.3 3,116,756 - -0.6 3,116,774 - PGA1_c29800 0.10 +0.9 3,116,791 + PGA1_c29800 0.12 -0.0 3,116,791 + PGA1_c29800 0.12 +0.3 3,116,791 + PGA1_c29800 0.12 -0.1 3,116,799 - PGA1_c29800 0.13 -1.5 3,116,799 - PGA1_c29800 0.13 -0.2 3,116,799 - PGA1_c29800 0.13 -1.9 3,116,799 - PGA1_c29800 0.13 -0.6 3,116,830 + PGA1_c29800 0.16 +1.0 3,116,871 - PGA1_c29800 0.20 -0.8 3,116,891 + PGA1_c29800 0.22 +1.3 3,116,899 + PGA1_c29800 0.23 -0.9 3,116,909 + PGA1_c29800 0.24 +0.1 3,116,909 + PGA1_c29800 0.24 +0.6 3,116,909 + PGA1_c29800 0.24 +0.6 3,116,909 + PGA1_c29800 0.24 -1.6 3,116,909 - PGA1_c29800 0.24 +0.2 3,116,917 - PGA1_c29800 0.24 -1.3 3,116,917 - PGA1_c29800 0.24 -0.8 3,116,966 - PGA1_c29800 0.29 +0.7 3,116,971 - PGA1_c29800 0.30 -1.0 3,116,981 - PGA1_c29800 0.31 +1.1 3,116,989 + PGA1_c29800 0.32 +1.2 3,117,012 + PGA1_c29800 0.34 -0.0 3,117,012 + PGA1_c29800 0.34 -0.2 3,117,018 + PGA1_c29800 0.35 -0.3 3,117,020 - PGA1_c29800 0.35 -1.0 3,117,020 - PGA1_c29800 0.35 -0.1 3,117,059 + PGA1_c29800 0.39 -3.0 3,117,067 + PGA1_c29800 0.40 -0.6 3,117,075 - PGA1_c29800 0.40 +0.1 3,117,080 - PGA1_c29800 0.41 +0.8 3,117,085 + PGA1_c29800 0.41 -0.3 3,117,118 + PGA1_c29800 0.45 -0.0 3,117,118 + PGA1_c29800 0.45 -1.0 3,117,118 + PGA1_c29800 0.45 +1.4 3,117,124 - PGA1_c29800 0.45 -0.5 3,117,128 + PGA1_c29800 0.46 +0.2 3,117,128 + PGA1_c29800 0.46 +0.3 3,117,136 - PGA1_c29800 0.47 -0.3 3,117,136 - PGA1_c29800 0.47 -0.1 3,117,183 - PGA1_c29800 0.51 -3.1 3,117,203 - PGA1_c29800 0.53 -0.2 3,117,247 + PGA1_c29800 0.58 -2.0 3,117,247 + PGA1_c29800 0.58 -0.8 3,117,255 - PGA1_c29800 0.59 -0.3 3,117,255 - PGA1_c29800 0.59 -1.0 3,117,271 - PGA1_c29800 0.60 -1.2 3,117,356 + PGA1_c29800 0.69 -0.9 3,117,449 + PGA1_c29800 0.78 -0.3 3,117,473 - PGA1_c29800 0.81 -1.8 3,117,580 + -0.2 3,117,588 - -1.1 3,117,615 + +0.8 3,117,615 + -0.4 3,117,615 + -2.0 3,117,615 + -1.1 3,117,615 + -1.0 3,117,623 - -0.9 3,117,623 - +0.7 3,117,623 - +0.4 3,117,623 - +0.9 3,117,659 + -2.3 3,117,696 + -2.5 3,117,696 + +0.0 3,117,701 + -0.8 3,117,718 + -2.8 3,117,944 - +0.5 3,117,980 + -0.6 3,117,988 - +0.6 3,118,003 + -0.8 3,118,011 - +1.7 3,118,047 + -0.4 3,118,047 + +0.7 3,118,047 + -0.3 3,118,055 - -0.2 3,118,150 - PGA1_c29810 0.14 -0.9 3,118,171 + PGA1_c29810 0.16 +0.0 3,118,171 + PGA1_c29810 0.16 -2.1 3,118,171 + PGA1_c29810 0.16 -0.1 3,118,171 + PGA1_c29810 0.16 -0.7 3,118,171 + PGA1_c29810 0.16 -0.4 3,118,234 + PGA1_c29810 0.24 -0.6 3,118,234 + PGA1_c29810 0.24 -0.2 3,118,234 + PGA1_c29810 0.24 +0.4 3,118,242 - PGA1_c29810 0.25 -0.1 3,118,264 + PGA1_c29810 0.27 +0.1 3,118,318 + PGA1_c29810 0.34 -0.8 3,118,331 - PGA1_c29810 0.35 +1.1 3,118,332 + PGA1_c29810 0.35 -0.2 3,118,339 + PGA1_c29810 0.36 +0.9 3,118,354 - PGA1_c29810 0.38 +0.5 3,118,449 + PGA1_c29810 0.50 -1.6 3,118,466 + PGA1_c29810 0.52 -0.5 3,118,467 - PGA1_c29810 0.52 -1.1 3,118,467 - PGA1_c29810 0.52 +0.9 3,118,467 - PGA1_c29810 0.52 -1.0 3,118,524 - PGA1_c29810 0.59 +0.7 3,118,564 - PGA1_c29810 0.63 -0.8 3,118,655 - PGA1_c29810 0.74 -0.4 3,118,661 - PGA1_c29810 0.75 -0.8
Or see this region's nucleotide sequence