Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3548 and Psest_3549 overlap by 4 nucleotides Psest_3549 and Psest_3550 are separated by 59 nucleotides Psest_3550 and Psest_3551 are separated by 11 nucleotides
Psest_3548: Psest_3548 - transcription antitermination factor NusB, at 3,727,746 to 3,728,231
_3548
Psest_3549: Psest_3549 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,728,228 to 3,728,704
_3549
Psest_3550: Psest_3550 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,728,764 to 3,729,843
_3550
Psest_3551: Psest_3551 - riboflavin synthase, alpha subunit, at 3,729,855 to 3,730,517
_3551
Position (kb)
3728
3729
3730 Strain fitness (log2 ratio)
-1
0
1 at 3728.781 kb on - strand at 3728.781 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3729.044 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3730.587 kb on - strand at 3730.587 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.661 kb on - strand at 3730.724 kb on - strand at 3730.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,728,781 - +0.6 3,728,781 - -1.6 3,728,786 - +1.4 3,728,786 - +1.2 3,728,786 - +0.2 3,728,786 - -0.4 3,729,044 - Psest_3550 0.26 -1.6 3,729,172 - Psest_3550 0.38 -0.4 3,729,172 - Psest_3550 0.38 -0.0 3,729,172 - Psest_3550 0.38 -0.6 3,730,587 - +0.2 3,730,587 - -1.2 3,730,661 - -0.7 3,730,661 - -0.8 3,730,661 - -0.4 3,730,661 - -0.2 3,730,661 - +0.4 3,730,724 - +0.5 3,730,812 + -0.5
Or see this region's nucleotide sequence