Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2214 and Psest_2215 are separated by 51 nucleotides Psest_2215 and Psest_2216 are separated by 259 nucleotides Psest_2216 and Psest_2217 are separated by 9 nucleotides Psest_2217 and Psest_2218 are separated by 13 nucleotides Psest_2218 and Psest_2219 overlap by 4 nucleotides
Psest_2214: Psest_2214 - hypothetical protein, at 2,364,847 to 2,365,149
_2214
Psest_2215: Psest_2215 - hypothetical protein, at 2,365,201 to 2,365,485
_2215
Psest_2216: Psest_2216 - Acyl carrier protein, at 2,365,745 to 2,365,996
_2216
Psest_2217: Psest_2217 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 2,366,006 to 2,367,154
_2217
Psest_2218: Psest_2218 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 2,367,168 to 2,368,142
_2218
Psest_2219: Psest_2219 - pyruvate dehydrogenase E1 component, alpha subunit, at 2,368,139 to 2,369,131
_2219
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-2
-1
0
1 at 2365.647 kb on + strand at 2365.647 kb on + strand at 2365.648 kb on - strand at 2365.747 kb on - strand at 2365.774 kb on - strand, within Psest_2216 at 2365.809 kb on + strand, within Psest_2216 at 2365.856 kb on + strand, within Psest_2216 at 2365.857 kb on - strand, within Psest_2216 at 2365.860 kb on + strand, within Psest_2216 at 2365.928 kb on + strand, within Psest_2216 at 2365.928 kb on + strand, within Psest_2216 at 2366.103 kb on - strand at 2366.103 kb on - strand at 2366.174 kb on + strand, within Psest_2217 at 2366.174 kb on + strand, within Psest_2217 at 2366.174 kb on + strand, within Psest_2217 at 2366.175 kb on - strand, within Psest_2217 at 2366.197 kb on - strand, within Psest_2217 at 2366.451 kb on + strand, within Psest_2217 at 2366.507 kb on + strand, within Psest_2217 at 2366.508 kb on - strand, within Psest_2217 at 2366.508 kb on - strand, within Psest_2217 at 2366.550 kb on + strand, within Psest_2217 at 2366.550 kb on + strand, within Psest_2217 at 2366.601 kb on + strand, within Psest_2217 at 2366.601 kb on + strand, within Psest_2217 at 2366.602 kb on - strand, within Psest_2217 at 2366.602 kb on - strand, within Psest_2217 at 2367.170 kb on - strand at 2367.610 kb on + strand, within Psest_2218 at 2367.610 kb on + strand, within Psest_2218 at 2367.611 kb on - strand, within Psest_2218 at 2368.027 kb on + strand, within Psest_2218 at 2368.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,365,647 + -0.7 2,365,647 + -2.5 2,365,648 - -0.8 2,365,747 - -1.1 2,365,774 - Psest_2216 0.12 -0.4 2,365,809 + Psest_2216 0.25 +0.8 2,365,856 + Psest_2216 0.44 -1.0 2,365,857 - Psest_2216 0.44 -1.0 2,365,860 + Psest_2216 0.46 -0.6 2,365,928 + Psest_2216 0.73 -0.1 2,365,928 + Psest_2216 0.73 +0.5 2,366,103 - -2.6 2,366,103 - -0.3 2,366,174 + Psest_2217 0.15 -0.5 2,366,174 + Psest_2217 0.15 +0.2 2,366,174 + Psest_2217 0.15 +1.4 2,366,175 - Psest_2217 0.15 -0.2 2,366,197 - Psest_2217 0.17 -0.0 2,366,451 + Psest_2217 0.39 -0.2 2,366,507 + Psest_2217 0.44 -0.8 2,366,508 - Psest_2217 0.44 -1.2 2,366,508 - Psest_2217 0.44 -0.9 2,366,550 + Psest_2217 0.47 +1.4 2,366,550 + Psest_2217 0.47 -1.2 2,366,601 + Psest_2217 0.52 +0.1 2,366,601 + Psest_2217 0.52 +1.4 2,366,602 - Psest_2217 0.52 +0.1 2,366,602 - Psest_2217 0.52 -0.9 2,367,170 - -0.2 2,367,610 + Psest_2218 0.45 -0.2 2,367,610 + Psest_2218 0.45 +0.7 2,367,611 - Psest_2218 0.45 +0.8 2,368,027 + Psest_2218 0.88 -0.5 2,368,124 - -1.2
Or see this region's nucleotide sequence