Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1907 and Psest_1908 are separated by 3 nucleotides Psest_1908 and Psest_1909 are separated by 3 nucleotides Psest_1909 and Psest_1910 overlap by 4 nucleotides Psest_1910 and Psest_1911 are separated by 187 nucleotides
Psest_1907: Psest_1907 - hypothetical protein, at 2,016,798 to 2,017,007
_1907
Psest_1908: Psest_1908 - hypothetical protein, at 2,017,011 to 2,017,205
_1908
Psest_1909: Psest_1909 - Phosphohistidine phosphatase SixA, at 2,017,209 to 2,017,766
_1909
Psest_1910: Psest_1910 - 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase, at 2,017,763 to 2,018,647
_1910
Psest_1911: Psest_1911 - spermidine synthase, at 2,018,835 to 2,019,695
_1911
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.765 kb on - strand at 2016.765 kb on - strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.773 kb on + strand at 2016.774 kb on - strand at 2016.848 kb on + strand, within Psest_1907 at 2017.129 kb on + strand, within Psest_1908 at 2017.203 kb on + strand at 2017.203 kb on + strand at 2017.312 kb on + strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.466 kb on + strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.741 kb on + strand at 2017.788 kb on + strand at 2017.789 kb on - strand at 2017.789 kb on - strand at 2017.959 kb on + strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2018.078 kb on + strand, within Psest_1910 at 2018.405 kb on + strand, within Psest_1910 at 2018.405 kb on + strand, within Psest_1910 at 2018.406 kb on - strand, within Psest_1910 at 2018.406 kb on - strand, within Psest_1910 at 2018.647 kb on + strand at 2018.659 kb on - strand at 2018.734 kb on + strand at 2018.734 kb on + strand at 2018.735 kb on - strand at 2018.735 kb on - strand at 2019.012 kb on + strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.348 kb on + strand, within Psest_1911 at 2019.480 kb on + strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.612 kb on + strand at 2019.613 kb on - strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.616 kb on - strand at 2019.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,016,764 + +0.0 2,016,764 + -1.2 2,016,764 + -0.8 2,016,764 + -0.4 2,016,765 - -0.5 2,016,765 - +0.6 2,016,769 + +0.7 2,016,769 + +0.4 2,016,769 + +1.4 2,016,769 + +1.4 2,016,770 - +0.9 2,016,770 - +0.8 2,016,770 - -0.7 2,016,770 - +0.8 2,016,770 - +0.3 2,016,770 - +0.2 2,016,773 + -0.4 2,016,774 - -0.4 2,016,848 + Psest_1907 0.24 -0.8 2,017,129 + Psest_1908 0.61 +0.8 2,017,203 + +0.8 2,017,203 + -0.0 2,017,312 + Psest_1909 0.18 +0.8 2,017,313 - Psest_1909 0.19 +1.8 2,017,313 - Psest_1909 0.19 -1.2 2,017,313 - Psest_1909 0.19 +0.3 2,017,466 + Psest_1909 0.46 -0.6 2,017,467 - Psest_1909 0.46 -0.2 2,017,467 - Psest_1909 0.46 -0.7 2,017,467 - Psest_1909 0.46 -0.4 2,017,741 + -1.0 2,017,788 + +0.3 2,017,789 - -0.5 2,017,789 - +0.8 2,017,959 + Psest_1910 0.22 -1.8 2,017,960 - Psest_1910 0.22 -0.5 2,017,960 - Psest_1910 0.22 -0.2 2,018,078 + Psest_1910 0.36 -0.5 2,018,405 + Psest_1910 0.73 -0.9 2,018,405 + Psest_1910 0.73 -0.5 2,018,406 - Psest_1910 0.73 +0.2 2,018,406 - Psest_1910 0.73 +0.2 2,018,647 + -0.4 2,018,659 - +1.5 2,018,734 + -0.5 2,018,734 + +0.6 2,018,735 - +1.8 2,018,735 - -0.8 2,019,012 + Psest_1911 0.21 -2.0 2,019,013 - Psest_1911 0.21 +0.1 2,019,013 - Psest_1911 0.21 -0.2 2,019,013 - Psest_1911 0.21 +2.1 2,019,013 - Psest_1911 0.21 -0.4 2,019,348 + Psest_1911 0.60 -1.5 2,019,480 + Psest_1911 0.75 +0.1 2,019,481 - Psest_1911 0.75 +0.7 2,019,481 - Psest_1911 0.75 +0.4 2,019,481 - Psest_1911 0.75 +0.3 2,019,497 + Psest_1911 0.77 +0.9 2,019,497 + Psest_1911 0.77 -0.8 2,019,497 + Psest_1911 0.77 +0.0 2,019,498 - Psest_1911 0.77 -0.4 2,019,498 - Psest_1911 0.77 -0.1 2,019,498 - Psest_1911 0.77 +2.8 2,019,501 + Psest_1911 0.77 +2.4 2,019,501 + Psest_1911 0.77 +0.8 2,019,501 + Psest_1911 0.77 +0.4 2,019,501 + Psest_1911 0.77 -1.2 2,019,501 + Psest_1911 0.77 -0.4 2,019,501 + Psest_1911 0.77 -0.8 2,019,502 - Psest_1911 0.77 +0.9 2,019,502 - Psest_1911 0.77 +0.4 2,019,502 - Psest_1911 0.77 -0.2 2,019,612 + -0.8 2,019,613 - +0.0 2,019,615 + +0.8 2,019,615 + +0.7 2,019,615 + +0.4 2,019,615 + +0.7 2,019,616 - +0.8 2,019,616 - +0.0
Or see this region's nucleotide sequence