Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06950 and PGA1_c06960 are separated by 99 nucleotides PGA1_c06960 and PGA1_c06970 are separated by 256 nucleotides PGA1_c06970 and PGA1_c06980 are separated by 35 nucleotides PGA1_c06980 and PGA1_c06990 are separated by 187 nucleotides PGA1_c06990 and PGA1_c07000 are separated by 15 nucleotides
PGA1_c06950: PGA1_c06950 - Uncharacterized conserved protein, at 715,740 to 716,171
_c06950
PGA1_c06960: PGA1_c06960 - Uncharacterized protein conserved in bacteria, at 716,271 to 716,588
_c06960
PGA1_c06970: PGA1_c06970 - pyruvate kinase PykA, at 716,845 to 718,290
_c06970
PGA1_c06980: PGA1_c06980 - hypothetical protein, at 718,326 to 718,547
_c06980
PGA1_c06990: PGA1_c06990 - 50S ribosomal protein L35, at 718,735 to 718,935
_c06990
PGA1_c07000: PGA1_c07000 - 50S ribosomal protein L20, at 718,951 to 719,316
_c07000
Position (kb) 716
717
718
719 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 715.862 kb on + strand, within PGA1_c06950 at 715.862 kb on + strand, within PGA1_c06950 at 715.862 kb on + strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.888 kb on - strand, within PGA1_c06950 at 715.889 kb on + strand, within PGA1_c06950 at 716.001 kb on + strand, within PGA1_c06950 at 716.039 kb on + strand, within PGA1_c06950 at 716.048 kb on + strand, within PGA1_c06950 at 716.056 kb on - strand, within PGA1_c06950 at 716.071 kb on + strand, within PGA1_c06950 at 716.086 kb on - strand, within PGA1_c06950 at 716.093 kb on - strand, within PGA1_c06950 at 716.104 kb on - strand, within PGA1_c06950 at 716.168 kb on - strand at 716.173 kb on - strand at 716.178 kb on - strand at 716.185 kb on + strand at 716.187 kb on + strand at 716.193 kb on - strand at 716.205 kb on + strand at 716.205 kb on + strand at 716.213 kb on - strand at 716.242 kb on + strand at 716.366 kb on + strand, within PGA1_c06960 at 716.388 kb on - strand, within PGA1_c06960 at 716.390 kb on + strand, within PGA1_c06960 at 716.441 kb on + strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.631 kb on + strand at 716.631 kb on + strand at 716.631 kb on + strand at 716.631 kb on + strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.641 kb on + strand at 716.644 kb on - strand at 716.661 kb on + strand at 716.695 kb on - strand at 716.717 kb on + strand at 716.750 kb on + strand at 716.752 kb on - strand at 716.835 kb on + strand at 718.282 kb on - strand at 718.367 kb on - strand, within PGA1_c06980 at 718.457 kb on + strand, within PGA1_c06980 at 718.460 kb on + strand, within PGA1_c06980 at 718.535 kb on - strand at 718.576 kb on + strand at 718.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 715,862 + PGA1_c06950 0.28 +0.1 715,862 + PGA1_c06950 0.28 +0.9 715,862 + PGA1_c06950 0.28 +0.8 715,870 - PGA1_c06950 0.30 -0.5 715,870 - PGA1_c06950 0.30 +0.1 715,870 - PGA1_c06950 0.30 -2.1 715,870 - PGA1_c06950 0.30 +3.2 715,888 - PGA1_c06950 0.34 +0.6 715,889 + PGA1_c06950 0.34 +1.1 716,001 + PGA1_c06950 0.60 +1.2 716,039 + PGA1_c06950 0.69 +0.4 716,048 + PGA1_c06950 0.71 -0.6 716,056 - PGA1_c06950 0.73 +1.6 716,071 + PGA1_c06950 0.77 +0.3 716,086 - PGA1_c06950 0.80 -0.4 716,093 - PGA1_c06950 0.82 +1.9 716,104 - PGA1_c06950 0.84 -0.1 716,168 - +0.2 716,173 - -0.7 716,178 - -0.3 716,185 + +0.5 716,187 + +0.8 716,193 - -0.9 716,205 + -0.7 716,205 + -1.7 716,213 - +0.0 716,242 + +0.4 716,366 + PGA1_c06960 0.30 -2.5 716,388 - PGA1_c06960 0.37 -0.7 716,390 + PGA1_c06960 0.37 -0.1 716,441 + PGA1_c06960 0.53 -2.5 716,449 - PGA1_c06960 0.56 +1.8 716,449 - PGA1_c06960 0.56 -1.7 716,449 - PGA1_c06960 0.56 -2.3 716,631 + -0.1 716,631 + +0.3 716,631 + +0.1 716,631 + +0.4 716,639 - +0.8 716,639 - -0.1 716,639 - -3.3 716,639 - -2.8 716,639 - +2.6 716,639 - +0.9 716,639 - +1.6 716,639 - -1.1 716,641 + +0.9 716,644 - +2.7 716,661 + -0.0 716,695 - +1.3 716,717 + +1.2 716,750 + +1.0 716,752 - -1.4 716,835 + -0.6 718,282 - +1.0 718,367 - PGA1_c06980 0.18 -2.1 718,457 + PGA1_c06980 0.59 -0.7 718,460 + PGA1_c06980 0.60 +0.1 718,535 - +0.5 718,576 + -1.4 718,586 + -0.9
Or see this region's nucleotide sequence