Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3777 and Psest_3778 are separated by 224 nucleotides Psest_3778 and Psest_3779 are separated by 97 nucleotides Psest_3779 and Psest_3780 are separated by 1 nucleotides
Psest_3777: Psest_3777 - diguanylate cyclase (GGDEF) domain, at 3,944,761 to 3,946,419
_3777
Psest_3778: Psest_3778 - ATP-dependent DNA helicase Rep, at 3,946,644 to 3,948,653
_3778
Psest_3779: Psest_3779 - channel protein, hemolysin III family, at 3,948,751 to 3,949,368
_3779
Psest_3780: Psest_3780 - Transcriptional regulator, at 3,949,370 to 3,950,158
_3780
Position (kb)
3946
3947
3948
3949 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3945.729 kb on - strand, within Psest_3777 at 3945.729 kb on - strand, within Psest_3777 at 3945.953 kb on + strand, within Psest_3777 at 3946.384 kb on + strand at 3946.385 kb on - strand at 3946.385 kb on - strand at 3946.385 kb on - strand at 3946.385 kb on - strand at 3946.446 kb on - strand at 3946.446 kb on - strand at 3946.446 kb on - strand at 3946.446 kb on - strand at 3946.519 kb on - strand at 3946.519 kb on - strand at 3946.526 kb on + strand at 3946.527 kb on - strand at 3946.527 kb on - strand at 3946.527 kb on - strand at 3946.567 kb on + strand at 3946.568 kb on - strand at 3946.608 kb on + strand at 3948.696 kb on - strand at 3948.754 kb on + strand at 3948.755 kb on - strand at 3948.755 kb on - strand at 3948.883 kb on + strand, within Psest_3779 at 3948.883 kb on + strand, within Psest_3779 at 3948.884 kb on - strand, within Psest_3779 at 3948.884 kb on - strand, within Psest_3779 at 3948.907 kb on + strand, within Psest_3779 at 3948.907 kb on + strand, within Psest_3779 at 3948.907 kb on + strand, within Psest_3779 at 3948.926 kb on - strand, within Psest_3779 at 3948.926 kb on - strand, within Psest_3779 at 3948.985 kb on + strand, within Psest_3779 at 3948.985 kb on + strand, within Psest_3779 at 3948.985 kb on + strand, within Psest_3779 at 3948.985 kb on + strand, within Psest_3779 at 3948.986 kb on - strand, within Psest_3779 at 3948.986 kb on - strand, within Psest_3779 at 3949.007 kb on - strand, within Psest_3779 at 3949.007 kb on - strand, within Psest_3779 at 3949.144 kb on + strand, within Psest_3779 at 3949.144 kb on + strand, within Psest_3779 at 3949.144 kb on + strand, within Psest_3779 at 3949.145 kb on - strand, within Psest_3779 at 3949.145 kb on - strand, within Psest_3779 at 3949.243 kb on + strand, within Psest_3779 at 3949.251 kb on - strand, within Psest_3779 at 3949.357 kb on + strand at 3949.357 kb on + strand at 3949.357 kb on + strand at 3949.357 kb on + strand at 3949.357 kb on + strand at 3949.358 kb on - strand at 3949.358 kb on - strand at 3949.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,945,729 - Psest_3777 0.58 -0.6 3,945,729 - Psest_3777 0.58 +0.2 3,945,953 + Psest_3777 0.72 +0.8 3,946,384 + -0.8 3,946,385 - -0.4 3,946,385 - +0.6 3,946,385 - -0.4 3,946,385 - -1.2 3,946,446 - +0.7 3,946,446 - +0.4 3,946,446 - +1.3 3,946,446 - +1.0 3,946,519 - +1.7 3,946,519 - +2.3 3,946,526 + +1.5 3,946,527 - +0.1 3,946,527 - +0.6 3,946,527 - +0.9 3,946,567 + -0.2 3,946,568 - +0.3 3,946,608 + -0.4 3,948,696 - -0.2 3,948,754 + -0.5 3,948,755 - +1.8 3,948,755 - +0.4 3,948,883 + Psest_3779 0.21 +0.4 3,948,883 + Psest_3779 0.21 -1.6 3,948,884 - Psest_3779 0.22 -0.6 3,948,884 - Psest_3779 0.22 -0.1 3,948,907 + Psest_3779 0.25 -2.3 3,948,907 + Psest_3779 0.25 +0.1 3,948,907 + Psest_3779 0.25 -0.2 3,948,926 - Psest_3779 0.28 -0.6 3,948,926 - Psest_3779 0.28 +0.3 3,948,985 + Psest_3779 0.38 +0.5 3,948,985 + Psest_3779 0.38 -0.0 3,948,985 + Psest_3779 0.38 -0.1 3,948,985 + Psest_3779 0.38 +0.0 3,948,986 - Psest_3779 0.38 -0.2 3,948,986 - Psest_3779 0.38 -0.3 3,949,007 - Psest_3779 0.41 -1.1 3,949,007 - Psest_3779 0.41 -0.4 3,949,144 + Psest_3779 0.64 -1.2 3,949,144 + Psest_3779 0.64 +2.2 3,949,144 + Psest_3779 0.64 +1.2 3,949,145 - Psest_3779 0.64 +0.5 3,949,145 - Psest_3779 0.64 -0.1 3,949,243 + Psest_3779 0.80 +0.5 3,949,251 - Psest_3779 0.81 -1.4 3,949,357 + -0.5 3,949,357 + -0.4 3,949,357 + +0.4 3,949,357 + -0.2 3,949,357 + +0.2 3,949,358 - -0.4 3,949,358 - +0.3 3,949,358 - -0.8
Or see this region's nucleotide sequence