Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3742 and Psest_3743 are separated by 104 nucleotides Psest_3743 and Psest_3744 are separated by 257 nucleotides Psest_3744 and Psest_3745 are separated by 117 nucleotides
Psest_3742: Psest_3742 - hypothetical protein, at 3,913,414 to 3,914,244
_3742
Psest_3743: Psest_3743 - Regulator of sigma D, at 3,914,349 to 3,914,816
_3743
Psest_3744: Psest_3744 - Disulfide bond formation protein DsbB, at 3,915,074 to 3,915,565
_3744
Psest_3745: Psest_3745 - Uncharacterized enzyme of heme biosynthesis, at 3,915,683 to 3,916,903
_3745
Position (kb)
3915
3916 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3914.217 kb on + strand at 3914.217 kb on + strand at 3914.277 kb on + strand at 3914.277 kb on + strand at 3914.277 kb on + strand at 3914.280 kb on + strand at 3914.280 kb on + strand at 3914.281 kb on - strand at 3914.281 kb on - strand at 3914.281 kb on - strand at 3914.360 kb on + strand at 3914.546 kb on - strand, within Psest_3743 at 3914.615 kb on - strand, within Psest_3743 at 3914.641 kb on + strand, within Psest_3743 at 3914.641 kb on + strand, within Psest_3743 at 3914.642 kb on - strand, within Psest_3743 at 3914.642 kb on - strand, within Psest_3743 at 3914.653 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.767 kb on - strand, within Psest_3743 at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.834 kb on - strand at 3914.849 kb on - strand at 3914.940 kb on + strand at 3914.940 kb on + strand at 3914.941 kb on - strand at 3915.063 kb on - strand at 3915.246 kb on + strand, within Psest_3744 at 3915.246 kb on + strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.341 kb on + strand, within Psest_3744 at 3915.360 kb on + strand, within Psest_3744 at 3915.360 kb on + strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.489 kb on + strand, within Psest_3744 at 3915.489 kb on + strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.594 kb on + strand at 3915.595 kb on - strand at 3915.620 kb on + strand at 3915.620 kb on + strand at 3915.621 kb on - strand at 3916.311 kb on + strand, within Psest_3745 at 3916.311 kb on + strand, within Psest_3745 at 3916.311 kb on + strand, within Psest_3745 at 3916.312 kb on - strand, within Psest_3745 at 3916.312 kb on - strand, within Psest_3745 at 3916.330 kb on + strand, within Psest_3745 at 3916.331 kb on - strand, within Psest_3745 at 3916.331 kb on - strand, within Psest_3745 at 3916.339 kb on - strand, within Psest_3745 at 3916.395 kb on + strand, within Psest_3745 at 3916.395 kb on + strand, within Psest_3745 at 3916.395 kb on + strand, within Psest_3745 at 3916.540 kb on - strand, within Psest_3745
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,914,217 + -0.1 3,914,217 + -0.6 3,914,277 + -0.1 3,914,277 + -0.3 3,914,277 + -2.6 3,914,280 + +0.0 3,914,280 + -0.4 3,914,281 - -1.1 3,914,281 - -1.3 3,914,281 - -0.3 3,914,360 + -0.3 3,914,546 - Psest_3743 0.42 +0.9 3,914,615 - Psest_3743 0.57 -0.2 3,914,641 + Psest_3743 0.62 +0.2 3,914,641 + Psest_3743 0.62 -1.0 3,914,642 - Psest_3743 0.63 -0.9 3,914,642 - Psest_3743 0.63 -0.1 3,914,653 + Psest_3743 0.65 +0.8 3,914,766 + Psest_3743 0.89 +0.7 3,914,766 + Psest_3743 0.89 -0.3 3,914,766 + Psest_3743 0.89 +0.3 3,914,766 + Psest_3743 0.89 +0.5 3,914,766 + Psest_3743 0.89 -0.7 3,914,767 - Psest_3743 0.89 -0.4 3,914,772 + -0.2 3,914,772 + -0.4 3,914,772 + -0.8 3,914,772 + +0.8 3,914,772 + -0.2 3,914,772 + +0.3 3,914,772 + +0.5 3,914,773 - +0.1 3,914,773 - -0.1 3,914,773 - +1.8 3,914,773 - +1.3 3,914,773 - +0.2 3,914,773 - -1.3 3,914,773 - -0.4 3,914,773 - -0.1 3,914,834 - +0.0 3,914,849 - -0.6 3,914,940 + +2.3 3,914,940 + +0.7 3,914,941 - -0.4 3,915,063 - -0.4 3,915,246 + Psest_3744 0.35 -1.9 3,915,246 + Psest_3744 0.35 -0.7 3,915,247 - Psest_3744 0.35 -0.6 3,915,247 - Psest_3744 0.35 -1.8 3,915,247 - Psest_3744 0.35 -0.3 3,915,247 - Psest_3744 0.35 +0.2 3,915,247 - Psest_3744 0.35 -0.6 3,915,247 - Psest_3744 0.35 -0.9 3,915,341 + Psest_3744 0.54 -0.8 3,915,360 + Psest_3744 0.58 -0.9 3,915,360 + Psest_3744 0.58 -2.2 3,915,361 - Psest_3744 0.58 -1.3 3,915,361 - Psest_3744 0.58 -1.6 3,915,361 - Psest_3744 0.58 -2.3 3,915,489 + Psest_3744 0.84 -0.8 3,915,489 + Psest_3744 0.84 -1.3 3,915,490 - Psest_3744 0.85 -0.7 3,915,490 - Psest_3744 0.85 +0.3 3,915,490 - Psest_3744 0.85 -0.9 3,915,490 - Psest_3744 0.85 -0.2 3,915,490 - Psest_3744 0.85 -0.8 3,915,594 + +0.7 3,915,595 - -0.3 3,915,620 + +0.5 3,915,620 + -0.5 3,915,621 - +0.7 3,916,311 + Psest_3745 0.51 -0.3 3,916,311 + Psest_3745 0.51 +0.4 3,916,311 + Psest_3745 0.51 +0.2 3,916,312 - Psest_3745 0.52 +0.8 3,916,312 - Psest_3745 0.52 -0.8 3,916,330 + Psest_3745 0.53 -0.8 3,916,331 - Psest_3745 0.53 +0.7 3,916,331 - Psest_3745 0.53 -1.2 3,916,339 - Psest_3745 0.54 -0.1 3,916,395 + Psest_3745 0.58 -0.2 3,916,395 + Psest_3745 0.58 -0.1 3,916,395 + Psest_3745 0.58 -2.5 3,916,540 - Psest_3745 0.70 -1.3
Or see this region's nucleotide sequence