Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2160 and Psest_2161 overlap by 4 nucleotides Psest_2161 and Psest_2162 overlap by 4 nucleotides Psest_2162 and Psest_2163 are separated by 383 nucleotides
Psest_2160: Psest_2160 - Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes, at 2,312,374 to 2,313,615
_2160
Psest_2161: Psest_2161 - Metal-dependent hydrolase, at 2,313,612 to 2,314,415
_2161
Psest_2162: Psest_2162 - Uncharacterized conserved protein, at 2,314,412 to 2,315,329
_2162
Psest_2163: Psest_2163 - Transcriptional regulator, at 2,315,713 to 2,316,633
_2163
Position (kb)
2314
2315
2316 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2313.475 kb on + strand, within Psest_2160 at 2313.608 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.615 kb on + strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.616 kb on - strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.617 kb on + strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.618 kb on - strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.621 kb on + strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.622 kb on - strand at 2313.678 kb on + strand at 2313.678 kb on + strand at 2313.678 kb on + strand at 2313.678 kb on + strand at 2313.679 kb on - strand at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.712 kb on + strand, within Psest_2161 at 2313.713 kb on - strand, within Psest_2161 at 2313.990 kb on + strand, within Psest_2161 at 2313.990 kb on + strand, within Psest_2161 at 2313.990 kb on + strand, within Psest_2161 at 2313.991 kb on - strand, within Psest_2161 at 2313.991 kb on - strand, within Psest_2161 at 2314.162 kb on + strand, within Psest_2161 at 2314.163 kb on - strand, within Psest_2161 at 2314.206 kb on - strand, within Psest_2161 at 2314.430 kb on + strand at 2314.430 kb on + strand at 2314.430 kb on + strand at 2314.430 kb on + strand at 2314.430 kb on + strand at 2314.431 kb on - strand at 2314.431 kb on - strand at 2314.431 kb on - strand at 2314.431 kb on - strand at 2314.530 kb on + strand, within Psest_2162 at 2314.530 kb on + strand, within Psest_2162 at 2314.536 kb on + strand, within Psest_2162 at 2314.537 kb on - strand, within Psest_2162 at 2314.537 kb on - strand, within Psest_2162 at 2314.671 kb on + strand, within Psest_2162 at 2314.671 kb on + strand, within Psest_2162 at 2314.671 kb on + strand, within Psest_2162 at 2314.672 kb on - strand, within Psest_2162 at 2314.672 kb on - strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.701 kb on + strand, within Psest_2162 at 2314.702 kb on - strand, within Psest_2162 at 2314.702 kb on - strand, within Psest_2162 at 2314.702 kb on - strand, within Psest_2162 at 2314.702 kb on - strand, within Psest_2162 at 2314.776 kb on + strand, within Psest_2162 at 2314.776 kb on + strand, within Psest_2162 at 2314.776 kb on + strand, within Psest_2162 at 2314.777 kb on - strand, within Psest_2162 at 2314.777 kb on - strand, within Psest_2162 at 2314.777 kb on - strand, within Psest_2162 at 2314.777 kb on - strand, within Psest_2162 at 2314.777 kb on - strand, within Psest_2162 at 2314.780 kb on + strand, within Psest_2162 at 2314.781 kb on - strand, within Psest_2162 at 2314.781 kb on - strand, within Psest_2162 at 2314.872 kb on + strand, within Psest_2162 at 2314.873 kb on - strand, within Psest_2162 at 2314.873 kb on - strand, within Psest_2162 at 2314.949 kb on + strand, within Psest_2162 at 2314.949 kb on + strand, within Psest_2162 at 2314.950 kb on - strand, within Psest_2162 at 2315.187 kb on + strand, within Psest_2162 at 2315.187 kb on + strand, within Psest_2162 at 2315.187 kb on + strand, within Psest_2162 at 2315.268 kb on + strand at 2315.268 kb on + strand at 2315.308 kb on + strand at 2315.308 kb on + strand at 2315.412 kb on - strand at 2315.412 kb on - strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.445 kb on + strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.446 kb on - strand at 2315.537 kb on + strand at 2315.538 kb on - strand at 2315.555 kb on + strand at 2315.556 kb on - strand at 2315.556 kb on - strand at 2315.556 kb on - strand at 2315.765 kb on + strand at 2315.765 kb on + strand at 2315.915 kb on + strand, within Psest_2163 at 2315.916 kb on - strand, within Psest_2163 at 2315.916 kb on - strand, within Psest_2163 at 2315.916 kb on - strand, within Psest_2163 at 2316.211 kb on - strand, within Psest_2163 at 2316.249 kb on + strand, within Psest_2163 at 2316.249 kb on + strand, within Psest_2163 at 2316.268 kb on + strand, within Psest_2163
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,313,475 + Psest_2160 0.89 -1.1 2,313,608 + -0.0 2,313,615 + -0.1 2,313,615 + -1.0 2,313,615 + +0.9 2,313,615 + -0.0 2,313,615 + +0.6 2,313,615 + +0.1 2,313,615 + +0.5 2,313,615 + -0.8 2,313,615 + -0.5 2,313,615 + +0.2 2,313,615 + -0.4 2,313,615 + +0.7 2,313,615 + +0.9 2,313,615 + +0.2 2,313,615 + +0.0 2,313,615 + +0.5 2,313,615 + +0.4 2,313,615 + -0.2 2,313,615 + -0.4 2,313,616 - -1.5 2,313,616 - +1.6 2,313,616 - -0.6 2,313,616 - +0.7 2,313,616 - +0.1 2,313,616 - +0.8 2,313,616 - -0.5 2,313,616 - -0.1 2,313,616 - +0.5 2,313,616 - +0.3 2,313,616 - +0.2 2,313,616 - -1.0 2,313,616 - -0.6 2,313,616 - +0.1 2,313,616 - +0.2 2,313,616 - -0.0 2,313,616 - +0.2 2,313,617 + +1.1 2,313,617 + -1.3 2,313,617 + -0.2 2,313,617 + +0.1 2,313,617 + +2.4 2,313,617 + +0.2 2,313,617 + -0.2 2,313,617 + -0.3 2,313,617 + -0.2 2,313,617 + -0.4 2,313,617 + -0.5 2,313,617 + -0.6 2,313,617 + +1.5 2,313,617 + +1.2 2,313,617 + -0.6 2,313,617 + +0.1 2,313,617 + +0.0 2,313,617 + +0.9 2,313,617 + -0.6 2,313,617 + -0.7 2,313,617 + -0.7 2,313,617 + -1.5 2,313,617 + -0.4 2,313,617 + +0.1 2,313,617 + -0.6 2,313,617 + -1.4 2,313,617 + +0.2 2,313,617 + -0.4 2,313,617 + +0.4 2,313,617 + -0.7 2,313,617 + -0.2 2,313,617 + -0.3 2,313,617 + -0.6 2,313,617 + +0.9 2,313,617 + +0.2 2,313,617 + -0.7 2,313,617 + -0.2 2,313,617 + +0.2 2,313,617 + +0.0 2,313,617 + +0.8 2,313,617 + -0.0 2,313,617 + -0.4 2,313,617 + -0.0 2,313,617 + -0.3 2,313,617 + -0.0 2,313,617 + -0.5 2,313,617 + +1.4 2,313,617 + -2.1 2,313,617 + -0.3 2,313,617 + -0.2 2,313,617 + -0.4 2,313,617 + -0.6 2,313,617 + +0.0 2,313,617 + -0.0 2,313,617 + -0.1 2,313,617 + -0.8 2,313,617 + -0.5 2,313,617 + -0.5 2,313,617 + +1.4 2,313,618 - -0.9 2,313,618 - +0.0 2,313,618 - -0.4 2,313,618 - -1.1 2,313,618 - +0.1 2,313,618 - +0.6 2,313,618 - -0.7 2,313,618 - -0.0 2,313,618 - -0.4 2,313,618 - -0.6 2,313,618 - -0.8 2,313,618 - -0.2 2,313,618 - +1.1 2,313,618 - +0.2 2,313,618 - -0.6 2,313,618 - -0.7 2,313,618 - +0.9 2,313,618 - +0.6 2,313,618 - -0.8 2,313,618 - -0.5 2,313,618 - -0.9 2,313,618 - -0.3 2,313,618 - -2.4 2,313,618 - +1.1 2,313,618 - -1.5 2,313,618 - -0.6 2,313,618 - -0.5 2,313,618 - -1.8 2,313,618 - -0.1 2,313,618 - -0.4 2,313,618 - -0.4 2,313,618 - +0.6 2,313,618 - -0.0 2,313,618 - -0.2 2,313,618 - -2.0 2,313,618 - -0.5 2,313,618 - -0.3 2,313,618 - +0.0 2,313,618 - -1.2 2,313,618 - -0.0 2,313,621 + -0.6 2,313,621 + -1.1 2,313,621 + -0.6 2,313,621 + -0.8 2,313,621 + +0.0 2,313,621 + -0.4 2,313,621 + +0.2 2,313,621 + +0.2 2,313,621 + +0.3 2,313,621 + -0.6 2,313,621 + +0.8 2,313,622 - -0.4 2,313,622 - -0.4 2,313,622 - -0.3 2,313,622 - +0.2 2,313,622 - +0.6 2,313,622 - -0.2 2,313,622 - +0.4 2,313,622 - +0.2 2,313,622 - -0.5 2,313,622 - +0.2 2,313,622 - +0.5 2,313,622 - -0.4 2,313,622 - -0.1 2,313,622 - -1.4 2,313,678 + +0.6 2,313,678 + -1.0 2,313,678 + +1.2 2,313,678 + +1.1 2,313,679 - -3.6 2,313,712 + Psest_2161 0.12 +0.6 2,313,712 + Psest_2161 0.12 +0.0 2,313,712 + Psest_2161 0.12 +2.0 2,313,712 + Psest_2161 0.12 -0.4 2,313,712 + Psest_2161 0.12 -0.1 2,313,712 + Psest_2161 0.12 -0.5 2,313,712 + Psest_2161 0.12 +0.9 2,313,712 + Psest_2161 0.12 +0.3 2,313,712 + Psest_2161 0.12 +0.8 2,313,712 + Psest_2161 0.12 -0.8 2,313,712 + Psest_2161 0.12 -1.0 2,313,712 + Psest_2161 0.12 +0.1 2,313,712 + Psest_2161 0.12 -0.2 2,313,713 - Psest_2161 0.13 -2.3 2,313,990 + Psest_2161 0.47 -0.8 2,313,990 + Psest_2161 0.47 +1.6 2,313,990 + Psest_2161 0.47 +0.0 2,313,991 - Psest_2161 0.47 -2.0 2,313,991 - Psest_2161 0.47 -0.5 2,314,162 + Psest_2161 0.68 +1.1 2,314,163 - Psest_2161 0.69 -0.3 2,314,206 - Psest_2161 0.74 -0.8 2,314,430 + +0.5 2,314,430 + -0.1 2,314,430 + +1.1 2,314,430 + -0.1 2,314,430 + +0.8 2,314,431 - -0.4 2,314,431 - -1.6 2,314,431 - +0.9 2,314,431 - -1.8 2,314,530 + Psest_2162 0.13 -1.1 2,314,530 + Psest_2162 0.13 -0.2 2,314,536 + Psest_2162 0.14 -0.1 2,314,537 - Psest_2162 0.14 -1.1 2,314,537 - Psest_2162 0.14 -1.2 2,314,671 + Psest_2162 0.28 -1.6 2,314,671 + Psest_2162 0.28 -0.4 2,314,671 + Psest_2162 0.28 -0.4 2,314,672 - Psest_2162 0.28 -1.3 2,314,672 - Psest_2162 0.28 -0.6 2,314,701 + Psest_2162 0.31 -0.4 2,314,701 + Psest_2162 0.31 -0.5 2,314,701 + Psest_2162 0.31 -0.9 2,314,701 + Psest_2162 0.31 -0.7 2,314,701 + Psest_2162 0.31 -0.3 2,314,701 + Psest_2162 0.31 -0.4 2,314,702 - Psest_2162 0.32 -2.0 2,314,702 - Psest_2162 0.32 -1.5 2,314,702 - Psest_2162 0.32 -3.3 2,314,702 - Psest_2162 0.32 -0.8 2,314,776 + Psest_2162 0.40 +1.7 2,314,776 + Psest_2162 0.40 +0.8 2,314,776 + Psest_2162 0.40 -1.2 2,314,777 - Psest_2162 0.40 +0.3 2,314,777 - Psest_2162 0.40 -2.2 2,314,777 - Psest_2162 0.40 -1.4 2,314,777 - Psest_2162 0.40 -1.6 2,314,777 - Psest_2162 0.40 -0.4 2,314,780 + Psest_2162 0.40 -0.8 2,314,781 - Psest_2162 0.40 -0.8 2,314,781 - Psest_2162 0.40 -0.8 2,314,872 + Psest_2162 0.50 +0.4 2,314,873 - Psest_2162 0.50 -0.2 2,314,873 - Psest_2162 0.50 -0.1 2,314,949 + Psest_2162 0.58 -1.1 2,314,949 + Psest_2162 0.58 +0.4 2,314,950 - Psest_2162 0.59 -0.2 2,315,187 + Psest_2162 0.84 +0.3 2,315,187 + Psest_2162 0.84 +0.1 2,315,187 + Psest_2162 0.84 -1.2 2,315,268 + -0.8 2,315,268 + -0.0 2,315,308 + -0.7 2,315,308 + -1.0 2,315,412 - -0.7 2,315,412 - -1.6 2,315,445 + -0.0 2,315,445 + +1.6 2,315,445 + +0.7 2,315,445 + -0.6 2,315,445 + +0.1 2,315,445 + +0.0 2,315,445 + +0.0 2,315,446 - -0.4 2,315,446 - -1.2 2,315,446 - -2.5 2,315,446 - -0.2 2,315,446 - -0.1 2,315,446 - -1.2 2,315,446 - -0.9 2,315,446 - -1.1 2,315,446 - -1.4 2,315,446 - -2.4 2,315,537 + -1.4 2,315,538 - -0.7 2,315,555 + +1.6 2,315,556 - +0.3 2,315,556 - -0.7 2,315,556 - -0.1 2,315,765 + -0.0 2,315,765 + +0.2 2,315,915 + Psest_2163 0.22 -0.0 2,315,916 - Psest_2163 0.22 +0.6 2,315,916 - Psest_2163 0.22 +0.5 2,315,916 - Psest_2163 0.22 -0.5 2,316,211 - Psest_2163 0.54 -0.5 2,316,249 + Psest_2163 0.58 +0.1 2,316,249 + Psest_2163 0.58 +0.6 2,316,268 + Psest_2163 0.60 -1.3
Or see this region's nucleotide sequence