Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1789 and Psest_1790 are separated by 174 nucleotides Psest_1790 and Psest_1791 overlap by 8 nucleotides Psest_1791 and Psest_1792 are separated by 4 nucleotides
Psest_1789: Psest_1789 - Zn-dependent hydrolases, including glyoxylases, at 1,884,233 to 1,885,096
_1789
Psest_1790: Psest_1790 - ABC-type antimicrobial peptide transport system, ATPase component, at 1,885,271 to 1,886,002
_1790
Psest_1791: Psest_1791 - ABC-type transport system, involved in lipoprotein release, permease component, at 1,885,995 to 1,887,215
_1791
Psest_1792: Psest_1792 - RND family efflux transporter, MFP subunit, at 1,887,220 to 1,888,440
_1792
Position (kb)
1885
1886
1887
1888 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1885.007 kb on + strand, within Psest_1789 at 1885.040 kb on + strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.041 kb on - strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.044 kb on + strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.045 kb on - strand at 1885.050 kb on + strand at 1885.050 kb on + strand at 1885.050 kb on + strand at 1885.050 kb on + strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.051 kb on - strand at 1885.101 kb on + strand at 1885.171 kb on + strand at 1885.190 kb on + strand at 1885.191 kb on - strand at 1885.776 kb on + strand, within Psest_1790 at 1885.776 kb on + strand, within Psest_1790 at 1885.776 kb on + strand, within Psest_1790 at 1885.777 kb on - strand, within Psest_1790 at 1885.777 kb on - strand, within Psest_1790 at 1885.819 kb on + strand, within Psest_1790 at 1885.820 kb on - strand, within Psest_1790 at 1885.824 kb on - strand, within Psest_1790 at 1885.824 kb on - strand, within Psest_1790 at 1885.828 kb on + strand, within Psest_1790 at 1885.828 kb on + strand, within Psest_1790 at 1885.828 kb on + strand, within Psest_1790 at 1885.828 kb on + strand, within Psest_1790 at 1885.828 kb on - strand, within Psest_1790 at 1885.829 kb on - strand, within Psest_1790 at 1885.879 kb on - strand, within Psest_1790 at 1885.946 kb on + strand at 1885.946 kb on + strand at 1885.947 kb on - strand at 1885.947 kb on - strand at 1886.064 kb on + strand at 1886.064 kb on + strand at 1886.155 kb on - strand, within Psest_1791 at 1886.155 kb on - strand, within Psest_1791 at 1886.155 kb on - strand, within Psest_1791 at 1886.404 kb on - strand, within Psest_1791 at 1886.566 kb on + strand, within Psest_1791 at 1886.567 kb on - strand, within Psest_1791 at 1886.567 kb on - strand, within Psest_1791 at 1886.567 kb on - strand, within Psest_1791 at 1886.572 kb on + strand, within Psest_1791 at 1886.573 kb on - strand, within Psest_1791 at 1886.573 kb on - strand, within Psest_1791 at 1887.397 kb on + strand, within Psest_1792 at 1887.398 kb on - strand, within Psest_1792 at 1887.400 kb on + strand, within Psest_1792 at 1887.400 kb on + strand, within Psest_1792 at 1887.400 kb on + strand, within Psest_1792 at 1887.401 kb on - strand, within Psest_1792 at 1887.401 kb on - strand, within Psest_1792 at 1887.742 kb on + strand, within Psest_1792 at 1887.742 kb on + strand, within Psest_1792 at 1887.743 kb on - strand, within Psest_1792 at 1887.787 kb on + strand, within Psest_1792 at 1887.850 kb on + strand, within Psest_1792
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,885,007 + Psest_1789 0.90 +0.1 1,885,040 + -0.1 1,885,041 - +1.2 1,885,041 - -0.0 1,885,041 - -0.5 1,885,041 - -0.3 1,885,041 - +0.3 1,885,041 - -0.1 1,885,041 - -0.8 1,885,041 - -0.6 1,885,041 - +0.7 1,885,041 - -0.7 1,885,041 - -0.2 1,885,041 - -1.3 1,885,044 + +0.5 1,885,044 + -0.1 1,885,044 + +0.0 1,885,044 + +0.9 1,885,044 + -0.5 1,885,044 + -0.4 1,885,044 + -1.5 1,885,044 + +0.6 1,885,044 + -0.8 1,885,044 + +0.0 1,885,044 + +0.3 1,885,044 + -0.3 1,885,044 + -1.0 1,885,044 + -0.2 1,885,044 + +0.4 1,885,044 + -0.6 1,885,044 + -0.1 1,885,044 + +0.2 1,885,045 - -1.8 1,885,045 - -0.0 1,885,045 - -0.1 1,885,045 - +0.2 1,885,045 - -0.8 1,885,045 - -0.5 1,885,045 - -0.7 1,885,045 - +1.2 1,885,045 - -0.1 1,885,045 - -2.1 1,885,045 - +0.6 1,885,045 - -1.6 1,885,050 + +3.1 1,885,050 + -0.3 1,885,050 + -0.8 1,885,050 + +0.7 1,885,051 - -0.1 1,885,051 - -1.9 1,885,051 - -1.2 1,885,051 - -0.0 1,885,051 - -0.9 1,885,051 - -0.4 1,885,051 - +0.7 1,885,051 - -0.0 1,885,051 - -0.2 1,885,051 - -0.1 1,885,101 + -0.1 1,885,171 + +0.6 1,885,190 + -0.3 1,885,191 - +0.2 1,885,776 + Psest_1790 0.69 -0.4 1,885,776 + Psest_1790 0.69 +0.1 1,885,776 + Psest_1790 0.69 -0.1 1,885,777 - Psest_1790 0.69 -0.3 1,885,777 - Psest_1790 0.69 +0.1 1,885,819 + Psest_1790 0.75 -0.0 1,885,820 - Psest_1790 0.75 +1.0 1,885,824 - Psest_1790 0.76 -0.2 1,885,824 - Psest_1790 0.76 +1.0 1,885,828 + Psest_1790 0.76 -0.6 1,885,828 + Psest_1790 0.76 -0.1 1,885,828 + Psest_1790 0.76 +0.2 1,885,828 + Psest_1790 0.76 +0.8 1,885,828 - Psest_1790 0.76 -0.4 1,885,829 - Psest_1790 0.76 +0.6 1,885,879 - Psest_1790 0.83 +0.5 1,885,946 + -0.3 1,885,946 + +1.1 1,885,947 - +0.5 1,885,947 - +0.7 1,886,064 + +0.2 1,886,064 + -0.1 1,886,155 - Psest_1791 0.13 +0.2 1,886,155 - Psest_1791 0.13 +0.2 1,886,155 - Psest_1791 0.13 -0.0 1,886,404 - Psest_1791 0.33 -1.0 1,886,566 + Psest_1791 0.47 -0.8 1,886,567 - Psest_1791 0.47 -0.4 1,886,567 - Psest_1791 0.47 -2.1 1,886,567 - Psest_1791 0.47 -0.3 1,886,572 + Psest_1791 0.47 +0.1 1,886,573 - Psest_1791 0.47 -0.8 1,886,573 - Psest_1791 0.47 -1.1 1,887,397 + Psest_1792 0.14 -1.3 1,887,398 - Psest_1792 0.15 +1.4 1,887,400 + Psest_1792 0.15 -0.3 1,887,400 + Psest_1792 0.15 +0.0 1,887,400 + Psest_1792 0.15 +0.9 1,887,401 - Psest_1792 0.15 -1.0 1,887,401 - Psest_1792 0.15 -0.1 1,887,742 + Psest_1792 0.43 +0.9 1,887,742 + Psest_1792 0.43 -0.3 1,887,743 - Psest_1792 0.43 -0.1 1,887,787 + Psest_1792 0.46 -0.6 1,887,850 + Psest_1792 0.52 -0.8
Or see this region's nucleotide sequence