Experiment: m.b. Benzethonium chloride 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03160 and PGA1_c03170 are separated by 24 nucleotides PGA1_c03170 and PGA1_c03180 are separated by 82 nucleotides PGA1_c03180 and PGA1_c03190 are separated by 88 nucleotides PGA1_c03190 and PGA1_c03200 are separated by 8 nucleotides PGA1_c03200 and PGA1_c03210 are separated by 79 nucleotides
PGA1_c03160: PGA1_c03160 - metallo-beta-lactamase-like protein, at 305,300 to 306,337
_c03160
PGA1_c03170: PGA1_c03170 - hypothetical protein, at 306,362 to 306,826
_c03170
PGA1_c03180: PGA1_c03180 - hypothetical protein, at 306,909 to 307,394
_c03180
PGA1_c03190: PGA1_c03190 - Bacterial aa3 type cytochrome c oxidase subunit IV., at 307,483 to 307,617
_c03190
PGA1_c03200: PGA1_c03200 - hypothetical protein, at 307,626 to 308,294
_c03200
PGA1_c03210: PGA1_c03210 - branched-chain amino acid transport protein azlD-like protein, at 308,374 to 308,709
_c03210
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 305.923 kb on - strand, within PGA1_c03160 at 306.014 kb on - strand, within PGA1_c03160 at 306.037 kb on + strand, within PGA1_c03160 at 306.047 kb on + strand, within PGA1_c03160 at 306.073 kb on - strand, within PGA1_c03160 at 306.099 kb on + strand, within PGA1_c03160 at 306.163 kb on + strand, within PGA1_c03160 at 306.233 kb on + strand, within PGA1_c03160 at 306.238 kb on + strand at 306.285 kb on - strand at 306.325 kb on + strand at 306.332 kb on + strand at 306.358 kb on + strand at 306.358 kb on + strand at 306.371 kb on - strand at 306.395 kb on + strand at 306.407 kb on + strand at 306.415 kb on - strand, within PGA1_c03170 at 306.425 kb on - strand, within PGA1_c03170 at 306.425 kb on - strand, within PGA1_c03170 at 306.436 kb on - strand, within PGA1_c03170 at 306.516 kb on + strand, within PGA1_c03170 at 306.524 kb on - strand, within PGA1_c03170 at 306.570 kb on - strand, within PGA1_c03170 at 306.584 kb on + strand, within PGA1_c03170 at 306.592 kb on - strand, within PGA1_c03170 at 306.676 kb on + strand, within PGA1_c03170 at 306.684 kb on - strand, within PGA1_c03170 at 306.832 kb on + strand at 306.837 kb on + strand at 306.900 kb on + strand at 307.016 kb on - strand, within PGA1_c03180 at 307.026 kb on - strand, within PGA1_c03180 at 307.039 kb on + strand, within PGA1_c03180 at 307.093 kb on + strand, within PGA1_c03180 at 307.145 kb on + strand, within PGA1_c03180 at 307.335 kb on + strand, within PGA1_c03180 at 307.346 kb on + strand at 307.423 kb on + strand at 307.423 kb on + strand at 307.423 kb on + strand at 307.431 kb on - strand at 307.571 kb on - strand, within PGA1_c03190 at 307.619 kb on - strand at 307.700 kb on - strand, within PGA1_c03200 at 307.700 kb on - strand, within PGA1_c03200 at 307.750 kb on - strand, within PGA1_c03200 at 307.843 kb on - strand, within PGA1_c03200 at 307.870 kb on + strand, within PGA1_c03200 at 307.981 kb on + strand, within PGA1_c03200 at 307.989 kb on - strand, within PGA1_c03200 at 308.020 kb on + strand, within PGA1_c03200 at 308.035 kb on - strand, within PGA1_c03200 at 308.054 kb on - strand, within PGA1_c03200 at 308.144 kb on - strand, within PGA1_c03200 at 308.194 kb on - strand, within PGA1_c03200 at 308.198 kb on + strand, within PGA1_c03200 at 308.234 kb on - strand at 308.264 kb on + strand at 308.272 kb on - strand at 308.272 kb on - strand at 308.306 kb on - strand at 308.367 kb on + strand at 308.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.025 mM remove 305,923 - PGA1_c03160 0.60 -1.7 306,014 - PGA1_c03160 0.69 +1.5 306,037 + PGA1_c03160 0.71 -2.9 306,047 + PGA1_c03160 0.72 -0.8 306,073 - PGA1_c03160 0.74 -2.0 306,099 + PGA1_c03160 0.77 -0.4 306,163 + PGA1_c03160 0.83 +1.1 306,233 + PGA1_c03160 0.90 -0.6 306,238 + -1.0 306,285 - +0.7 306,325 + -1.5 306,332 + +0.6 306,358 + +0.5 306,358 + -0.1 306,371 - +0.1 306,395 + +2.2 306,407 + -1.0 306,415 - PGA1_c03170 0.11 +0.5 306,425 - PGA1_c03170 0.14 +0.3 306,425 - PGA1_c03170 0.14 -0.0 306,436 - PGA1_c03170 0.16 +0.8 306,516 + PGA1_c03170 0.33 -1.0 306,524 - PGA1_c03170 0.35 +1.4 306,570 - PGA1_c03170 0.45 +0.9 306,584 + PGA1_c03170 0.48 -1.6 306,592 - PGA1_c03170 0.49 +0.2 306,676 + PGA1_c03170 0.68 +0.2 306,684 - PGA1_c03170 0.69 +1.0 306,832 + -2.0 306,837 + -2.7 306,900 + -1.1 307,016 - PGA1_c03180 0.22 -1.0 307,026 - PGA1_c03180 0.24 -0.8 307,039 + PGA1_c03180 0.27 -2.0 307,093 + PGA1_c03180 0.38 -3.2 307,145 + PGA1_c03180 0.49 +0.8 307,335 + PGA1_c03180 0.88 +1.2 307,346 + -0.8 307,423 + -2.6 307,423 + +1.5 307,423 + -1.6 307,431 - +0.9 307,571 - PGA1_c03190 0.65 -0.6 307,619 - +0.5 307,700 - PGA1_c03200 0.11 +1.8 307,700 - PGA1_c03200 0.11 -0.6 307,750 - PGA1_c03200 0.19 +2.0 307,843 - PGA1_c03200 0.32 +1.2 307,870 + PGA1_c03200 0.36 +2.7 307,981 + PGA1_c03200 0.53 +2.5 307,989 - PGA1_c03200 0.54 -2.0 308,020 + PGA1_c03200 0.59 -1.1 308,035 - PGA1_c03200 0.61 -1.8 308,054 - PGA1_c03200 0.64 +2.4 308,144 - PGA1_c03200 0.77 +2.5 308,194 - PGA1_c03200 0.85 +1.8 308,198 + PGA1_c03200 0.86 +2.1 308,234 - +1.5 308,264 + +1.7 308,272 - +3.8 308,272 - +2.2 308,306 - +1.0 308,367 + -0.0 308,375 - +2.4
Or see this region's nucleotide sequence