Experiment: m.b. Benzethonium chloride 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12270 and PGA1_c12280 are separated by 264 nucleotides PGA1_c12280 and PGA1_c12290 overlap by 1 nucleotides PGA1_c12290 and PGA1_c12300 are separated by 106 nucleotides
PGA1_c12270: PGA1_c12270 - monovalent cation/proton antiporter subunit A/B, at 1,271,917 to 1,274,778
_c12270
PGA1_c12280: PGA1_c12280 - Rhodanese-related sulfurtransferase, at 1,275,043 to 1,275,606
_c12280
PGA1_c12290: PGA1_c12290 - Poly(3-hydroxybutyrate) depolymerase, at 1,275,606 to 1,276,466
_c12290
PGA1_c12300: PGA1_c12300 - glucans biosynthesis glucosyltransferase H, at 1,276,573 to 1,278,483
_c12300
Position (kb) 1275
1276
1277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1274.866 kb on - strand at 1274.910 kb on + strand at 1274.915 kb on + strand at 1274.925 kb on + strand at 1274.963 kb on + strand at 1274.971 kb on - strand at 1275.023 kb on + strand at 1275.026 kb on + strand at 1275.083 kb on - strand at 1275.252 kb on - strand, within PGA1_c12280 at 1275.282 kb on + strand, within PGA1_c12280 at 1275.282 kb on + strand, within PGA1_c12280 at 1275.290 kb on - strand, within PGA1_c12280 at 1275.295 kb on - strand, within PGA1_c12280 at 1275.315 kb on - strand, within PGA1_c12280 at 1275.406 kb on - strand, within PGA1_c12280 at 1275.444 kb on + strand, within PGA1_c12280 at 1275.463 kb on + strand, within PGA1_c12280 at 1275.540 kb on - strand, within PGA1_c12280 at 1275.550 kb on + strand at 1275.603 kb on - strand at 1275.692 kb on + strand, within PGA1_c12290 at 1275.781 kb on + strand, within PGA1_c12290 at 1275.894 kb on + strand, within PGA1_c12290 at 1276.012 kb on - strand, within PGA1_c12290 at 1276.062 kb on - strand, within PGA1_c12290 at 1276.203 kb on - strand, within PGA1_c12290 at 1276.260 kb on + strand, within PGA1_c12290 at 1276.440 kb on + strand at 1276.440 kb on + strand at 1276.448 kb on - strand at 1276.448 kb on - strand at 1276.724 kb on - strand at 1276.761 kb on - strand at 1276.795 kb on + strand, within PGA1_c12300 at 1276.795 kb on + strand, within PGA1_c12300 at 1276.859 kb on - strand, within PGA1_c12300 at 1276.922 kb on - strand, within PGA1_c12300 at 1276.922 kb on - strand, within PGA1_c12300 at 1276.953 kb on - strand, within PGA1_c12300 at 1277.007 kb on + strand, within PGA1_c12300 at 1277.007 kb on + strand, within PGA1_c12300 at 1277.099 kb on + strand, within PGA1_c12300 at 1277.142 kb on + strand, within PGA1_c12300 at 1277.160 kb on - strand, within PGA1_c12300 at 1277.185 kb on + strand, within PGA1_c12300 at 1277.193 kb on - strand, within PGA1_c12300 at 1277.193 kb on - strand, within PGA1_c12300 at 1277.210 kb on + strand, within PGA1_c12300 at 1277.309 kb on - strand, within PGA1_c12300 at 1277.466 kb on - strand, within PGA1_c12300
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.025 mM remove 1,274,866 - -1.3 1,274,910 + +0.8 1,274,915 + -0.9 1,274,925 + -1.0 1,274,963 + -1.5 1,274,971 - +3.0 1,275,023 + -1.0 1,275,026 + +1.6 1,275,083 - -0.3 1,275,252 - PGA1_c12280 0.37 -0.5 1,275,282 + PGA1_c12280 0.42 -2.8 1,275,282 + PGA1_c12280 0.42 +1.2 1,275,290 - PGA1_c12280 0.44 -1.8 1,275,295 - PGA1_c12280 0.45 -0.7 1,275,315 - PGA1_c12280 0.48 +2.6 1,275,406 - PGA1_c12280 0.64 -0.5 1,275,444 + PGA1_c12280 0.71 -2.2 1,275,463 + PGA1_c12280 0.74 -1.5 1,275,540 - PGA1_c12280 0.88 +1.2 1,275,550 + +1.4 1,275,603 - -0.4 1,275,692 + PGA1_c12290 0.10 +1.5 1,275,781 + PGA1_c12290 0.20 -1.1 1,275,894 + PGA1_c12290 0.33 +0.0 1,276,012 - PGA1_c12290 0.47 +1.6 1,276,062 - PGA1_c12290 0.53 -0.3 1,276,203 - PGA1_c12290 0.69 +0.4 1,276,260 + PGA1_c12290 0.76 +1.2 1,276,440 + -1.7 1,276,440 + +1.1 1,276,448 - +1.5 1,276,448 - -2.7 1,276,724 - -2.6 1,276,761 - -2.8 1,276,795 + PGA1_c12300 0.12 -1.1 1,276,795 + PGA1_c12300 0.12 -2.5 1,276,859 - PGA1_c12300 0.15 -1.0 1,276,922 - PGA1_c12300 0.18 +0.7 1,276,922 - PGA1_c12300 0.18 -3.3 1,276,953 - PGA1_c12300 0.20 -1.1 1,277,007 + PGA1_c12300 0.23 -2.5 1,277,007 + PGA1_c12300 0.23 -2.3 1,277,099 + PGA1_c12300 0.28 -1.5 1,277,142 + PGA1_c12300 0.30 -2.5 1,277,160 - PGA1_c12300 0.31 -0.0 1,277,185 + PGA1_c12300 0.32 -1.2 1,277,193 - PGA1_c12300 0.32 -3.0 1,277,193 - PGA1_c12300 0.32 -3.3 1,277,210 + PGA1_c12300 0.33 -3.1 1,277,309 - PGA1_c12300 0.39 -0.6 1,277,466 - PGA1_c12300 0.47 -2.0
Or see this region's nucleotide sequence