Experiment: Sisomicin 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 53 nucleotides rbfA and Sama_0963 overlap by 1 nucleotides Sama_0963 and Sama_R0037 are separated by 41 nucleotides
Sama_0961: infB - translation initiation factor IF-2 (RefSeq), at 1,161,111 to 1,163,759
infB
Sama_0962: rbfA - ribosome-binding factor A (RefSeq), at 1,163,813 to 1,164,226
rbfA
Sama_0963: Sama_0963 - pseudouridylate synthase (RefSeq), at 1,164,226 to 1,165,173
_0963
Sama_R0037: Sama_R0037 - ribosomal S15 leader (NCBI), at 1,165,215 to 1,165,328
_R0037
Position (kb)
1163
1164
1165 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1164.319 kb on - strand at 1164.328 kb on + strand, within Sama_0963 at 1164.328 kb on + strand, within Sama_0963 at 1164.328 kb on + strand, within Sama_0963 at 1164.328 kb on + strand, within Sama_0963 at 1164.328 kb on + strand, within Sama_0963 at 1164.329 kb on - strand, within Sama_0963 at 1164.329 kb on - strand, within Sama_0963 at 1164.352 kb on - strand, within Sama_0963 at 1164.352 kb on - strand, within Sama_0963 at 1164.449 kb on - strand, within Sama_0963 at 1164.457 kb on - strand, within Sama_0963 at 1164.524 kb on + strand, within Sama_0963 at 1164.524 kb on + strand, within Sama_0963 at 1164.577 kb on - strand, within Sama_0963 at 1164.609 kb on + strand, within Sama_0963 at 1164.609 kb on + strand, within Sama_0963 at 1164.609 kb on + strand, within Sama_0963 at 1164.610 kb on - strand, within Sama_0963 at 1164.610 kb on - strand, within Sama_0963 at 1164.613 kb on + strand, within Sama_0963 at 1164.613 kb on + strand, within Sama_0963 at 1164.613 kb on + strand, within Sama_0963 at 1164.631 kb on + strand, within Sama_0963 at 1164.631 kb on + strand, within Sama_0963 at 1164.631 kb on + strand, within Sama_0963 at 1164.632 kb on - strand, within Sama_0963 at 1164.632 kb on - strand, within Sama_0963 at 1164.632 kb on - strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.633 kb on + strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.634 kb on - strand, within Sama_0963 at 1164.637 kb on + strand, within Sama_0963 at 1164.637 kb on + strand, within Sama_0963 at 1164.637 kb on + strand, within Sama_0963 at 1164.637 kb on + strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.638 kb on - strand, within Sama_0963 at 1164.652 kb on - strand, within Sama_0963 at 1164.696 kb on + strand, within Sama_0963 at 1164.735 kb on + strand, within Sama_0963 at 1164.753 kb on + strand, within Sama_0963 at 1164.753 kb on + strand, within Sama_0963 at 1164.753 kb on + strand, within Sama_0963 at 1164.753 kb on + strand, within Sama_0963 at 1164.754 kb on - strand, within Sama_0963 at 1164.754 kb on - strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.757 kb on + strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.758 kb on - strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.761 kb on + strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.762 kb on - strand, within Sama_0963 at 1164.765 kb on + strand, within Sama_0963 at 1164.765 kb on + strand, within Sama_0963 at 1164.765 kb on + strand, within Sama_0963 at 1164.765 kb on + strand, within Sama_0963 at 1164.765 kb on + strand, within Sama_0963 at 1164.766 kb on - strand, within Sama_0963 at 1164.766 kb on - strand, within Sama_0963 at 1164.816 kb on + strand, within Sama_0963 at 1164.816 kb on + strand, within Sama_0963 at 1164.817 kb on - strand, within Sama_0963 at 1164.817 kb on - strand, within Sama_0963 at 1164.817 kb on - strand, within Sama_0963 at 1164.850 kb on + strand, within Sama_0963 at 1164.858 kb on + strand, within Sama_0963 at 1164.858 kb on + strand, within Sama_0963 at 1164.859 kb on - strand, within Sama_0963 at 1164.859 kb on - strand, within Sama_0963 at 1164.859 kb on - strand, within Sama_0963 at 1164.859 kb on - strand, within Sama_0963 at 1164.859 kb on - strand, within Sama_0963 at 1164.988 kb on - strand, within Sama_0963 at 1164.988 kb on - strand, within Sama_0963 at 1165.009 kb on + strand, within Sama_0963 at 1165.068 kb on + strand, within Sama_0963 at 1165.068 kb on + strand, within Sama_0963 at 1165.068 kb on + strand, within Sama_0963 at 1165.069 kb on - strand, within Sama_0963 at 1165.071 kb on + strand, within Sama_0963 at 1165.072 kb on - strand, within Sama_0963 at 1165.072 kb on - strand, within Sama_0963 at 1165.156 kb on + strand at 1165.171 kb on + strand at 1165.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.00390625 mg/ml remove 1,164,319 - -0.0 1,164,328 + Sama_0963 0.11 -1.0 1,164,328 + Sama_0963 0.11 -0.8 1,164,328 + Sama_0963 0.11 +0.9 1,164,328 + Sama_0963 0.11 +0.7 1,164,328 + Sama_0963 0.11 -0.0 1,164,329 - Sama_0963 0.11 +0.3 1,164,329 - Sama_0963 0.11 -0.3 1,164,352 - Sama_0963 0.13 -0.2 1,164,352 - Sama_0963 0.13 +0.5 1,164,449 - Sama_0963 0.24 +0.8 1,164,457 - Sama_0963 0.24 +1.8 1,164,524 + Sama_0963 0.31 +0.3 1,164,524 + Sama_0963 0.31 -0.7 1,164,577 - Sama_0963 0.37 -0.5 1,164,609 + Sama_0963 0.40 -1.0 1,164,609 + Sama_0963 0.40 +1.8 1,164,609 + Sama_0963 0.40 +1.1 1,164,610 - Sama_0963 0.41 +0.1 1,164,610 - Sama_0963 0.41 +0.1 1,164,613 + Sama_0963 0.41 +0.5 1,164,613 + Sama_0963 0.41 +0.1 1,164,613 + Sama_0963 0.41 +0.7 1,164,631 + Sama_0963 0.43 +0.3 1,164,631 + Sama_0963 0.43 +0.2 1,164,631 + Sama_0963 0.43 +0.7 1,164,632 - Sama_0963 0.43 +1.2 1,164,632 - Sama_0963 0.43 -0.0 1,164,632 - Sama_0963 0.43 +1.2 1,164,633 + Sama_0963 0.43 -0.4 1,164,633 + Sama_0963 0.43 +1.6 1,164,633 + Sama_0963 0.43 +0.4 1,164,633 + Sama_0963 0.43 +0.5 1,164,633 + Sama_0963 0.43 +1.0 1,164,633 + Sama_0963 0.43 -0.3 1,164,633 + Sama_0963 0.43 -1.1 1,164,633 + Sama_0963 0.43 +1.3 1,164,633 + Sama_0963 0.43 +0.8 1,164,633 + Sama_0963 0.43 -0.0 1,164,633 + Sama_0963 0.43 -0.4 1,164,633 + Sama_0963 0.43 +0.5 1,164,633 + Sama_0963 0.43 +1.6 1,164,633 + Sama_0963 0.43 -1.2 1,164,634 - Sama_0963 0.43 +1.1 1,164,634 - Sama_0963 0.43 +0.2 1,164,634 - Sama_0963 0.43 +1.0 1,164,634 - Sama_0963 0.43 +0.7 1,164,634 - Sama_0963 0.43 -0.0 1,164,634 - Sama_0963 0.43 -0.5 1,164,634 - Sama_0963 0.43 +1.1 1,164,634 - Sama_0963 0.43 -0.0 1,164,634 - Sama_0963 0.43 -0.1 1,164,637 + Sama_0963 0.43 -1.0 1,164,637 + Sama_0963 0.43 +0.4 1,164,637 + Sama_0963 0.43 -0.6 1,164,637 + Sama_0963 0.43 -0.0 1,164,638 - Sama_0963 0.43 +0.4 1,164,638 - Sama_0963 0.43 -0.2 1,164,638 - Sama_0963 0.43 +0.6 1,164,638 - Sama_0963 0.43 +0.9 1,164,638 - Sama_0963 0.43 +1.1 1,164,638 - Sama_0963 0.43 +0.4 1,164,638 - Sama_0963 0.43 +0.5 1,164,638 - Sama_0963 0.43 -0.5 1,164,638 - Sama_0963 0.43 +1.0 1,164,638 - Sama_0963 0.43 +0.9 1,164,652 - Sama_0963 0.45 +1.2 1,164,696 + Sama_0963 0.50 +0.0 1,164,735 + Sama_0963 0.54 +0.3 1,164,753 + Sama_0963 0.56 +1.5 1,164,753 + Sama_0963 0.56 +0.4 1,164,753 + Sama_0963 0.56 +2.0 1,164,753 + Sama_0963 0.56 +1.0 1,164,754 - Sama_0963 0.56 +1.4 1,164,754 - Sama_0963 0.56 +1.3 1,164,757 + Sama_0963 0.56 +0.1 1,164,757 + Sama_0963 0.56 +1.2 1,164,757 + Sama_0963 0.56 +0.9 1,164,757 + Sama_0963 0.56 +1.2 1,164,757 + Sama_0963 0.56 -0.4 1,164,757 + Sama_0963 0.56 -0.7 1,164,757 + Sama_0963 0.56 +0.6 1,164,757 + Sama_0963 0.56 +0.6 1,164,757 + Sama_0963 0.56 +0.9 1,164,757 + Sama_0963 0.56 +0.6 1,164,757 + Sama_0963 0.56 +0.5 1,164,757 + Sama_0963 0.56 +1.6 1,164,757 + Sama_0963 0.56 -0.4 1,164,757 + Sama_0963 0.56 +0.8 1,164,757 + Sama_0963 0.56 +0.8 1,164,757 + Sama_0963 0.56 +0.3 1,164,757 + Sama_0963 0.56 +1.6 1,164,757 + Sama_0963 0.56 +0.4 1,164,757 + Sama_0963 0.56 -0.1 1,164,757 + Sama_0963 0.56 -0.4 1,164,757 + Sama_0963 0.56 +0.9 1,164,757 + Sama_0963 0.56 -2.6 1,164,757 + Sama_0963 0.56 +1.3 1,164,757 + Sama_0963 0.56 +0.4 1,164,757 + Sama_0963 0.56 +0.5 1,164,757 + Sama_0963 0.56 +0.8 1,164,757 + Sama_0963 0.56 +1.6 1,164,757 + Sama_0963 0.56 +1.6 1,164,757 + Sama_0963 0.56 +0.6 1,164,757 + Sama_0963 0.56 +2.6 1,164,757 + Sama_0963 0.56 +0.4 1,164,757 + Sama_0963 0.56 +0.4 1,164,757 + Sama_0963 0.56 +0.1 1,164,757 + Sama_0963 0.56 +2.2 1,164,757 + Sama_0963 0.56 -0.4 1,164,757 + Sama_0963 0.56 +2.3 1,164,757 + Sama_0963 0.56 +0.4 1,164,757 + Sama_0963 0.56 +1.2 1,164,758 - Sama_0963 0.56 +0.1 1,164,758 - Sama_0963 0.56 +0.8 1,164,758 - Sama_0963 0.56 +0.3 1,164,758 - Sama_0963 0.56 +0.3 1,164,758 - Sama_0963 0.56 -0.4 1,164,758 - Sama_0963 0.56 +0.8 1,164,758 - Sama_0963 0.56 +0.6 1,164,758 - Sama_0963 0.56 +0.7 1,164,758 - Sama_0963 0.56 -0.0 1,164,758 - Sama_0963 0.56 +0.8 1,164,758 - Sama_0963 0.56 +0.2 1,164,758 - Sama_0963 0.56 +0.3 1,164,758 - Sama_0963 0.56 +1.3 1,164,758 - Sama_0963 0.56 +1.9 1,164,758 - Sama_0963 0.56 +0.3 1,164,758 - Sama_0963 0.56 +0.4 1,164,758 - Sama_0963 0.56 -0.4 1,164,758 - Sama_0963 0.56 +0.8 1,164,758 - Sama_0963 0.56 -0.5 1,164,758 - Sama_0963 0.56 -1.1 1,164,758 - Sama_0963 0.56 +1.0 1,164,758 - Sama_0963 0.56 -0.6 1,164,758 - Sama_0963 0.56 +0.4 1,164,758 - Sama_0963 0.56 +0.9 1,164,758 - Sama_0963 0.56 +0.6 1,164,758 - Sama_0963 0.56 -0.7 1,164,758 - Sama_0963 0.56 +0.1 1,164,758 - Sama_0963 0.56 +0.9 1,164,758 - Sama_0963 0.56 +0.8 1,164,758 - Sama_0963 0.56 +0.4 1,164,758 - Sama_0963 0.56 +0.6 1,164,758 - Sama_0963 0.56 +1.0 1,164,758 - Sama_0963 0.56 +1.7 1,164,758 - Sama_0963 0.56 +0.9 1,164,761 + Sama_0963 0.56 +0.9 1,164,761 + Sama_0963 0.56 -0.0 1,164,761 + Sama_0963 0.56 -0.2 1,164,761 + Sama_0963 0.56 -0.9 1,164,761 + Sama_0963 0.56 +0.4 1,164,761 + Sama_0963 0.56 +1.0 1,164,761 + Sama_0963 0.56 +0.3 1,164,761 + Sama_0963 0.56 +1.5 1,164,761 + Sama_0963 0.56 +0.4 1,164,761 + Sama_0963 0.56 +1.1 1,164,761 + Sama_0963 0.56 +0.6 1,164,761 + Sama_0963 0.56 +0.9 1,164,761 + Sama_0963 0.56 +1.8 1,164,761 + Sama_0963 0.56 -1.6 1,164,761 + Sama_0963 0.56 +0.4 1,164,761 + Sama_0963 0.56 -0.4 1,164,761 + Sama_0963 0.56 +1.2 1,164,761 + Sama_0963 0.56 +1.4 1,164,762 - Sama_0963 0.57 +0.4 1,164,762 - Sama_0963 0.57 +0.8 1,164,762 - Sama_0963 0.57 -1.4 1,164,762 - Sama_0963 0.57 +0.8 1,164,762 - Sama_0963 0.57 -0.1 1,164,762 - Sama_0963 0.57 +1.7 1,164,762 - Sama_0963 0.57 -0.5 1,164,762 - Sama_0963 0.57 +0.8 1,164,762 - Sama_0963 0.57 +1.3 1,164,762 - Sama_0963 0.57 -0.9 1,164,762 - Sama_0963 0.57 +1.6 1,164,762 - Sama_0963 0.57 -0.0 1,164,762 - Sama_0963 0.57 -1.7 1,164,762 - Sama_0963 0.57 +1.4 1,164,762 - Sama_0963 0.57 -1.0 1,164,762 - Sama_0963 0.57 +1.9 1,164,762 - Sama_0963 0.57 -1.4 1,164,762 - Sama_0963 0.57 +1.6 1,164,762 - Sama_0963 0.57 +1.6 1,164,765 + Sama_0963 0.57 +1.4 1,164,765 + Sama_0963 0.57 +0.3 1,164,765 + Sama_0963 0.57 +0.9 1,164,765 + Sama_0963 0.57 -0.7 1,164,765 + Sama_0963 0.57 +0.8 1,164,766 - Sama_0963 0.57 +0.1 1,164,766 - Sama_0963 0.57 +1.0 1,164,816 + Sama_0963 0.62 +0.4 1,164,816 + Sama_0963 0.62 +0.9 1,164,817 - Sama_0963 0.62 -0.1 1,164,817 - Sama_0963 0.62 +0.6 1,164,817 - Sama_0963 0.62 +0.4 1,164,850 + Sama_0963 0.66 -0.1 1,164,858 + Sama_0963 0.67 +0.7 1,164,858 + Sama_0963 0.67 +0.8 1,164,859 - Sama_0963 0.67 +0.8 1,164,859 - Sama_0963 0.67 +0.5 1,164,859 - Sama_0963 0.67 +1.6 1,164,859 - Sama_0963 0.67 +0.1 1,164,859 - Sama_0963 0.67 -0.0 1,164,988 - Sama_0963 0.80 +0.3 1,164,988 - Sama_0963 0.80 +0.2 1,165,009 + Sama_0963 0.83 +0.4 1,165,068 + Sama_0963 0.89 -0.1 1,165,068 + Sama_0963 0.89 -0.4 1,165,068 + Sama_0963 0.89 -0.1 1,165,069 - Sama_0963 0.89 -0.5 1,165,071 + Sama_0963 0.89 +0.4 1,165,072 - Sama_0963 0.89 +0.3 1,165,072 - Sama_0963 0.89 -0.2 1,165,156 + +0.9 1,165,171 + +0.3 1,165,172 - -0.4
Or see this region's nucleotide sequence