Experiment: m.b. Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01440 and PGA1_c01460 are separated by 569 nucleotides PGA1_c01460 and PGA1_c01470 overlap by 4 nucleotides PGA1_c01470 and PGA1_c01480 are separated by 6 nucleotides
PGA1_c01440: PGA1_c01440 - DNA-directed RNA polymerase subunit beta', at 137,926 to 142,167
_c01440
PGA1_c01460: PGA1_c01460 - Protein of unknown function (DUF3118)., at 142,737 to 143,561
_c01460
PGA1_c01470: PGA1_c01470 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 143,558 to 144,460
_c01470
PGA1_c01480: PGA1_c01480 - Protein of unknown function (DUF3118)., at 144,467 to 145,243
_c01480
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 142.079 kb on + strand at 142.148 kb on + strand at 142.203 kb on - strand at 142.250 kb on + strand at 142.251 kb on - strand at 142.273 kb on - strand at 142.344 kb on - strand at 142.367 kb on + strand at 142.375 kb on - strand at 142.375 kb on - strand at 142.376 kb on + strand at 142.376 kb on + strand at 142.384 kb on - strand at 142.384 kb on - strand at 142.392 kb on - strand at 142.438 kb on - strand at 142.438 kb on - strand at 142.516 kb on - strand at 142.584 kb on - strand at 142.623 kb on - strand at 142.630 kb on - strand at 142.649 kb on - strand at 142.720 kb on + strand at 142.720 kb on + strand at 142.720 kb on + strand at 142.723 kb on - strand at 142.728 kb on - strand at 142.761 kb on + strand at 142.774 kb on + strand at 142.774 kb on + strand at 142.782 kb on - strand at 142.787 kb on - strand at 142.806 kb on - strand at 142.900 kb on + strand, within PGA1_c01460 at 142.991 kb on + strand, within PGA1_c01460 at 142.991 kb on + strand, within PGA1_c01460 at 143.008 kb on + strand, within PGA1_c01460 at 143.016 kb on - strand, within PGA1_c01460 at 143.070 kb on - strand, within PGA1_c01460 at 143.123 kb on - strand, within PGA1_c01460 at 143.172 kb on + strand, within PGA1_c01460 at 143.180 kb on - strand, within PGA1_c01460 at 143.180 kb on - strand, within PGA1_c01460 at 143.181 kb on + strand, within PGA1_c01460 at 143.210 kb on - strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.227 kb on + strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.235 kb on - strand, within PGA1_c01460 at 143.245 kb on + strand, within PGA1_c01460 at 143.372 kb on - strand, within PGA1_c01460 at 143.374 kb on + strand, within PGA1_c01460 at 143.382 kb on + strand, within PGA1_c01460 at 143.405 kb on + strand, within PGA1_c01460 at 143.410 kb on + strand, within PGA1_c01460 at 143.413 kb on - strand, within PGA1_c01460 at 143.462 kb on + strand, within PGA1_c01460 at 143.470 kb on - strand, within PGA1_c01460 at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.505 kb on + strand at 143.513 kb on - strand at 143.513 kb on - strand at 143.604 kb on - strand at 143.618 kb on - strand at 143.641 kb on + strand at 143.641 kb on + strand at 143.649 kb on - strand, within PGA1_c01470 at 143.649 kb on - strand, within PGA1_c01470 at 143.679 kb on + strand, within PGA1_c01470 at 143.687 kb on - strand, within PGA1_c01470 at 143.778 kb on + strand, within PGA1_c01470 at 143.786 kb on + strand, within PGA1_c01470 at 143.787 kb on + strand, within PGA1_c01470 at 143.788 kb on + strand, within PGA1_c01470 at 143.831 kb on + strand, within PGA1_c01470 at 143.834 kb on + strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.842 kb on - strand, within PGA1_c01470 at 143.956 kb on - strand, within PGA1_c01470 at 143.983 kb on - strand, within PGA1_c01470 at 144.020 kb on + strand, within PGA1_c01470 at 144.022 kb on - strand, within PGA1_c01470 at 144.028 kb on - strand, within PGA1_c01470 at 144.054 kb on + strand, within PGA1_c01470 at 144.120 kb on - strand, within PGA1_c01470 at 144.136 kb on - strand, within PGA1_c01470 at 144.136 kb on - strand, within PGA1_c01470 at 144.147 kb on + strand, within PGA1_c01470 at 144.155 kb on - strand, within PGA1_c01470 at 144.155 kb on - strand, within PGA1_c01470 at 144.165 kb on - strand, within PGA1_c01470 at 144.173 kb on - strand, within PGA1_c01470 at 144.173 kb on - strand, within PGA1_c01470 at 144.209 kb on + strand, within PGA1_c01470 at 144.210 kb on - strand, within PGA1_c01470 at 144.216 kb on + strand, within PGA1_c01470 at 144.435 kb on + strand at 144.435 kb on + strand at 144.548 kb on + strand, within PGA1_c01480
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloride 600 mM remove 142,079 + -2.9 142,148 + -1.2 142,203 - +1.3 142,250 + -1.0 142,251 - +0.0 142,273 - +0.0 142,344 - +2.0 142,367 + +2.9 142,375 - +0.3 142,375 - +1.0 142,376 + +0.6 142,376 + +0.2 142,384 - -3.4 142,384 - +0.2 142,392 - -0.5 142,438 - +0.5 142,438 - -2.2 142,516 - -0.7 142,584 - +0.5 142,623 - +0.1 142,630 - +0.6 142,649 - +0.3 142,720 + +1.9 142,720 + -0.8 142,720 + -0.2 142,723 - -0.5 142,728 - +0.6 142,761 + -0.1 142,774 + -0.1 142,774 + +0.6 142,782 - -0.1 142,787 - -1.1 142,806 - -0.3 142,900 + PGA1_c01460 0.20 -1.8 142,991 + PGA1_c01460 0.31 +1.0 142,991 + PGA1_c01460 0.31 +0.6 143,008 + PGA1_c01460 0.33 +0.9 143,016 - PGA1_c01460 0.34 -1.9 143,070 - PGA1_c01460 0.40 -1.9 143,123 - PGA1_c01460 0.47 +1.0 143,172 + PGA1_c01460 0.53 +1.0 143,180 - PGA1_c01460 0.54 -0.4 143,180 - PGA1_c01460 0.54 -0.3 143,181 + PGA1_c01460 0.54 +1.0 143,210 - PGA1_c01460 0.57 -0.0 143,227 + PGA1_c01460 0.59 -0.2 143,227 + PGA1_c01460 0.59 +2.1 143,227 + PGA1_c01460 0.59 -1.8 143,235 - PGA1_c01460 0.60 -0.9 143,235 - PGA1_c01460 0.60 +0.4 143,235 - PGA1_c01460 0.60 -0.3 143,235 - PGA1_c01460 0.60 +1.5 143,245 + PGA1_c01460 0.62 +0.8 143,372 - PGA1_c01460 0.77 +0.7 143,374 + PGA1_c01460 0.77 +0.3 143,382 + PGA1_c01460 0.78 -2.1 143,405 + PGA1_c01460 0.81 +0.9 143,410 + PGA1_c01460 0.82 -0.2 143,413 - PGA1_c01460 0.82 -0.0 143,462 + PGA1_c01460 0.88 +1.2 143,470 - PGA1_c01460 0.89 -0.4 143,505 + -1.9 143,505 + +1.2 143,505 + +0.8 143,505 + +1.2 143,505 + +0.6 143,505 + -0.9 143,513 - +0.3 143,513 - -0.5 143,604 - -1.9 143,618 - -0.1 143,641 + +2.2 143,641 + +0.4 143,649 - PGA1_c01470 0.10 -2.6 143,649 - PGA1_c01470 0.10 +0.7 143,679 + PGA1_c01470 0.13 +0.9 143,687 - PGA1_c01470 0.14 -0.3 143,778 + PGA1_c01470 0.24 -2.6 143,786 + PGA1_c01470 0.25 -0.2 143,787 + PGA1_c01470 0.25 +0.1 143,788 + PGA1_c01470 0.25 -2.3 143,831 + PGA1_c01470 0.30 +0.8 143,834 + PGA1_c01470 0.31 -0.6 143,842 - PGA1_c01470 0.31 -1.1 143,842 - PGA1_c01470 0.31 +3.1 143,842 - PGA1_c01470 0.31 -1.2 143,842 - PGA1_c01470 0.31 -1.3 143,956 - PGA1_c01470 0.44 +0.7 143,983 - PGA1_c01470 0.47 +1.3 144,020 + PGA1_c01470 0.51 -0.2 144,022 - PGA1_c01470 0.51 -0.7 144,028 - PGA1_c01470 0.52 -1.3 144,054 + PGA1_c01470 0.55 -0.3 144,120 - PGA1_c01470 0.62 -0.4 144,136 - PGA1_c01470 0.64 -0.2 144,136 - PGA1_c01470 0.64 +0.7 144,147 + PGA1_c01470 0.65 -0.8 144,155 - PGA1_c01470 0.66 -1.3 144,155 - PGA1_c01470 0.66 -1.4 144,165 - PGA1_c01470 0.67 -1.1 144,173 - PGA1_c01470 0.68 -2.7 144,173 - PGA1_c01470 0.68 -0.2 144,209 + PGA1_c01470 0.72 -0.6 144,210 - PGA1_c01470 0.72 +2.1 144,216 + PGA1_c01470 0.73 -0.4 144,435 + +1.2 144,435 + +0.7 144,548 + PGA1_c01480 0.10 +1.9
Or see this region's nucleotide sequence