Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1949 and Sama_1950 are separated by 56 nucleotides Sama_1950 and ihfA are separated by 81 nucleotides ihfA and pheT are separated by 3 nucleotides
Sama_1949: Sama_1949 - diguanylate cyclase, putative (RefSeq), at 2,376,276 to 2,377,259
_1949
Sama_1950: Sama_1950 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (RefSeq), at 2,377,316 to 2,378,242
_1950
Sama_1951: ihfA - integration host factor subunit alpha (RefSeq), at 2,378,324 to 2,378,620
ihfA
Sama_1952: pheT - phenylalanyl-tRNA synthetase subunit beta (RefSeq), at 2,378,624 to 2,381,011
pheT
Position (kb)
2377
2378
2379 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.348 kb on + strand at 2376.392 kb on + strand, within Sama_1949 at 2376.435 kb on - strand, within Sama_1949 at 2376.468 kb on + strand, within Sama_1949 at 2376.534 kb on + strand, within Sama_1949 at 2376.537 kb on - strand, within Sama_1949 at 2376.572 kb on + strand, within Sama_1949 at 2376.830 kb on - strand, within Sama_1949 at 2376.866 kb on + strand, within Sama_1949 at 2376.867 kb on - strand, within Sama_1949 at 2376.897 kb on - strand, within Sama_1949 at 2376.932 kb on + strand, within Sama_1949 at 2377.113 kb on + strand, within Sama_1949 at 2377.195 kb on - strand at 2377.195 kb on - strand at 2377.277 kb on - strand at 2377.290 kb on + strand at 2377.290 kb on + strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.292 kb on + strand at 2377.324 kb on - strand at 2377.376 kb on - strand at 2377.378 kb on - strand at 2377.611 kb on - strand, within Sama_1950 at 2377.710 kb on + strand, within Sama_1950 at 2377.768 kb on + strand, within Sama_1950 at 2377.832 kb on + strand, within Sama_1950 at 2377.842 kb on - strand, within Sama_1950 at 2378.051 kb on - strand, within Sama_1950 at 2378.254 kb on - strand at 2378.265 kb on - strand at 2378.541 kb on - strand, within ihfA at 2378.541 kb on - strand, within ihfA at 2378.541 kb on - strand, within ihfA at 2378.626 kb on - strand at 2378.626 kb on - strand at 2378.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 2,376,326 + +0.5 2,376,326 + +0.8 2,376,326 + -0.8 2,376,326 + -0.3 2,376,326 + +0.6 2,376,326 + -0.3 2,376,327 - +1.8 2,376,327 - -1.1 2,376,327 - -1.3 2,376,327 - -0.7 2,376,327 - -1.1 2,376,327 - +0.3 2,376,327 - -1.1 2,376,327 - -0.1 2,376,327 - -2.4 2,376,327 - -2.4 2,376,327 - +0.0 2,376,348 + -0.2 2,376,392 + Sama_1949 0.12 +0.6 2,376,435 - Sama_1949 0.16 +0.2 2,376,468 + Sama_1949 0.20 -0.9 2,376,534 + Sama_1949 0.26 -0.2 2,376,537 - Sama_1949 0.27 -0.1 2,376,572 + Sama_1949 0.30 -1.1 2,376,830 - Sama_1949 0.56 -0.4 2,376,866 + Sama_1949 0.60 -0.0 2,376,867 - Sama_1949 0.60 +0.5 2,376,897 - Sama_1949 0.63 +0.1 2,376,932 + Sama_1949 0.67 -0.1 2,377,113 + Sama_1949 0.85 -0.4 2,377,195 - -1.4 2,377,195 - -0.9 2,377,277 - -0.4 2,377,290 + -0.8 2,377,290 + +0.3 2,377,291 - -0.1 2,377,291 - -0.2 2,377,291 - +2.2 2,377,291 - +0.7 2,377,291 - +0.5 2,377,291 - -0.0 2,377,292 + -0.1 2,377,324 - -2.1 2,377,376 - -0.4 2,377,378 - -1.8 2,377,611 - Sama_1950 0.32 -1.0 2,377,710 + Sama_1950 0.43 -0.6 2,377,768 + Sama_1950 0.49 -1.0 2,377,832 + Sama_1950 0.56 -2.0 2,377,842 - Sama_1950 0.57 -1.4 2,378,051 - Sama_1950 0.79 -1.4 2,378,254 - +0.2 2,378,265 - +1.3 2,378,541 - ihfA Sama_1951 0.73 +4.1 2,378,541 - ihfA Sama_1951 0.73 +2.8 2,378,541 - ihfA Sama_1951 0.73 +2.8 2,378,626 - +3.2 2,378,626 - +2.4 2,378,626 - +4.1
Or see this region's nucleotide sequence