Strain Fitness in Shewanella amazonensis SB2B around Sama_1223

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSama_1222 and Sama_R0046 are separated by 237 nucleotidesSama_R0046 and Sama_R0047 are separated by 64 nucleotidesSama_R0047 and Sama_R0048 are separated by 12 nucleotidesSama_R0048 and tig are separated by 252 nucleotidestig and clpP are separated by 89 nucleotidesclpP and clpX are separated by 85 nucleotides Sama_1222: Sama_1222 - methylenetetrahydrofolate dehydrogenase (NADP(+)) (RefSeq), at 1,503,327 to 1,504,181 _1222 Sama_R0046: Sama_R0046 - tRNA-Pro (RefSeq), at 1,504,419 to 1,504,495 _R0046 Sama_R0047: Sama_R0047 - tRNA-Arg (RefSeq), at 1,504,560 to 1,504,636 _R0047 Sama_R0048: Sama_R0048 - tRNA-His (RefSeq), at 1,504,649 to 1,504,724 _R0048 Sama_1223: tig - trigger factor (RefSeq), at 1,504,977 to 1,506,281 tig Sama_1224: clpP - ATP-dependent Clp protease proteolytic subunit (RefSeq), at 1,506,371 to 1,506,979 clpP Sama_1225: clpX - ATP-dependent protease ATP-binding subunit ClpX (RefSeq), at 1,507,065 to 1,508,342 clpX Position (kb) 1504 1505 1506 1507Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1504.303 kb on + strandat 1504.304 kb on - strandat 1504.312 kb on - strandat 1504.313 kb on + strandat 1504.314 kb on - strandat 1504.484 kb on + strand, within Sama_R0046at 1504.499 kb on + strandat 1504.499 kb on + strandat 1504.501 kb on + strandat 1504.501 kb on + strandat 1504.501 kb on + strandat 1504.515 kb on + strandat 1504.554 kb on + strandat 1504.554 kb on + strandat 1504.739 kb on + strandat 1504.774 kb on + strandat 1504.815 kb on + strandat 1504.815 kb on + strandat 1504.919 kb on + strandat 1504.919 kb on + strandat 1504.919 kb on + strandat 1504.919 kb on + strandat 1504.920 kb on - strandat 1504.923 kb on - strandat 1504.942 kb on + strandat 1504.943 kb on - strandat 1504.954 kb on + strandat 1504.954 kb on + strandat 1504.955 kb on - strandat 1504.960 kb on + strandat 1504.960 kb on + strandat 1504.960 kb on + strandat 1504.961 kb on - strandat 1505.060 kb on + strandat 1505.118 kb on - strand, within tigat 1505.122 kb on + strand, within tigat 1505.122 kb on + strand, within tigat 1505.126 kb on + strand, within tigat 1505.126 kb on + strand, within tigat 1505.126 kb on + strand, within tigat 1505.148 kb on + strand, within tigat 1505.246 kb on + strand, within tigat 1505.246 kb on + strand, within tigat 1505.317 kb on + strand, within tigat 1505.317 kb on + strand, within tigat 1505.420 kb on + strand, within tigat 1505.420 kb on + strand, within tigat 1505.420 kb on + strand, within tigat 1505.420 kb on + strand, within tigat 1505.420 kb on + strand, within tigat 1505.552 kb on + strand, within tigat 1505.634 kb on + strand, within tigat 1505.634 kb on + strand, within tigat 1505.716 kb on + strand, within tigat 1505.716 kb on + strand, within tigat 1505.770 kb on + strand, within tigat 1505.911 kb on + strand, within tigat 1505.911 kb on + strand, within tigat 1505.911 kb on + strand, within tigat 1506.044 kb on + strand, within tigat 1506.092 kb on + strand, within tigat 1506.092 kb on + strand, within tigat 1506.132 kb on + strand, within tigat 1506.135 kb on + strand, within tigat 1506.135 kb on + strand, within tigat 1506.254 kb on + strandat 1506.254 kb on + strandat 1506.314 kb on + strandat 1506.314 kb on + strandat 1506.326 kb on + strandat 1506.326 kb on + strandat 1506.353 kb on + strandat 1506.449 kb on + strand, within clpPat 1506.449 kb on + strand, within clpPat 1506.450 kb on - strand, within clpPat 1506.450 kb on - strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.458 kb on + strand, within clpPat 1506.459 kb on - strand, within clpPat 1506.459 kb on - strand, within clpPat 1506.578 kb on + strand, within clpPat 1506.578 kb on + strand, within clpPat 1506.578 kb on + strand, within clpPat 1506.579 kb on - strand, within clpPat 1506.584 kb on + strand, within clpPat 1506.585 kb on - strand, within clpPat 1506.585 kb on - strand, within clpPat 1506.629 kb on + strand, within clpPat 1506.634 kb on + strand, within clpPat 1506.637 kb on + strand, within clpPat 1506.637 kb on + strand, within clpPat 1506.637 kb on + strand, within clpPat 1506.637 kb on + strand, within clpPat 1506.637 kb on + strand, within clpPat 1506.638 kb on - strand, within clpPat 1506.691 kb on + strand, within clpPat 1506.691 kb on + strand, within clpPat 1506.859 kb on - strand, within clpPat 1506.944 kb on + strandat 1507.023 kb on + strandat 1507.104 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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1,504,303 + -0.7
1,504,304 - +0.3
1,504,312 - +0.2
1,504,313 + +0.3
1,504,314 - +0.1
1,504,484 + Sama_R0046 0.84 +0.8
1,504,499 + -0.7
1,504,499 + -0.7
1,504,501 + -1.8
1,504,501 + -1.6
1,504,501 + +0.8
1,504,515 + -0.3
1,504,554 + -1.7
1,504,554 + -0.8
1,504,739 + +0.5
1,504,774 + +0.3
1,504,815 + -0.6
1,504,815 + -1.1
1,504,919 + -1.9
1,504,919 + -0.6
1,504,919 + -0.6
1,504,919 + -1.1
1,504,920 - -1.7
1,504,923 - +0.0
1,504,942 + -2.6
1,504,943 - +0.3
1,504,954 + -1.1
1,504,954 + -0.6
1,504,955 - -1.6
1,504,960 + -1.6
1,504,960 + -0.1
1,504,960 + -1.3
1,504,961 - -1.6
1,505,060 + -1.1
1,505,118 - tig Sama_1223 0.11 -1.3
1,505,122 + tig Sama_1223 0.11 -2.7
1,505,122 + tig Sama_1223 0.11 -2.4
1,505,126 + tig Sama_1223 0.11 +0.7
1,505,126 + tig Sama_1223 0.11 -0.7
1,505,126 + tig Sama_1223 0.11 -0.6
1,505,148 + tig Sama_1223 0.13 -2.2
1,505,246 + tig Sama_1223 0.21 -2.7
1,505,246 + tig Sama_1223 0.21 +0.3
1,505,317 + tig Sama_1223 0.26 +0.4
1,505,317 + tig Sama_1223 0.26 -1.9
1,505,420 + tig Sama_1223 0.34 -2.4
1,505,420 + tig Sama_1223 0.34 -0.2
1,505,420 + tig Sama_1223 0.34 -1.9
1,505,420 + tig Sama_1223 0.34 -1.0
1,505,420 + tig Sama_1223 0.34 -1.1
1,505,552 + tig Sama_1223 0.44 -2.3
1,505,634 + tig Sama_1223 0.50 -1.6
1,505,634 + tig Sama_1223 0.50 -3.3
1,505,716 + tig Sama_1223 0.57 -1.4
1,505,716 + tig Sama_1223 0.57 +1.0
1,505,770 + tig Sama_1223 0.61 -0.7
1,505,911 + tig Sama_1223 0.72 -1.9
1,505,911 + tig Sama_1223 0.72 -2.3
1,505,911 + tig Sama_1223 0.72 -1.6
1,506,044 + tig Sama_1223 0.82 -1.6
1,506,092 + tig Sama_1223 0.85 -2.4
1,506,092 + tig Sama_1223 0.85 -2.1
1,506,132 + tig Sama_1223 0.89 -0.7
1,506,135 + tig Sama_1223 0.89 -0.7
1,506,135 + tig Sama_1223 0.89 -1.7
1,506,254 + -1.1
1,506,254 + +0.4
1,506,314 + -1.1
1,506,314 + -0.6
1,506,326 + -1.3
1,506,326 + -1.1
1,506,353 + +3.1
1,506,449 + clpP Sama_1224 0.13 -1.7
1,506,449 + clpP Sama_1224 0.13 -0.2
1,506,450 - clpP Sama_1224 0.13 -2.0
1,506,450 - clpP Sama_1224 0.13 -2.7
1,506,458 + clpP Sama_1224 0.14 +0.8
1,506,458 + clpP Sama_1224 0.14 +0.4
1,506,458 + clpP Sama_1224 0.14 +2.5
1,506,458 + clpP Sama_1224 0.14 -0.0
1,506,458 + clpP Sama_1224 0.14 -1.3
1,506,458 + clpP Sama_1224 0.14 -2.2
1,506,459 - clpP Sama_1224 0.14 -0.9
1,506,459 - clpP Sama_1224 0.14 -2.1
1,506,578 + clpP Sama_1224 0.34 -2.7
1,506,578 + clpP Sama_1224 0.34 +2.3
1,506,578 + clpP Sama_1224 0.34 -0.7
1,506,579 - clpP Sama_1224 0.34 -0.6
1,506,584 + clpP Sama_1224 0.35 +1.1
1,506,585 - clpP Sama_1224 0.35 -1.2
1,506,585 - clpP Sama_1224 0.35 +1.3
1,506,629 + clpP Sama_1224 0.42 -1.7
1,506,634 + clpP Sama_1224 0.43 +3.6
1,506,637 + clpP Sama_1224 0.44 -1.1
1,506,637 + clpP Sama_1224 0.44 +0.3
1,506,637 + clpP Sama_1224 0.44 -0.6
1,506,637 + clpP Sama_1224 0.44 -2.1
1,506,637 + clpP Sama_1224 0.44 -0.3
1,506,638 - clpP Sama_1224 0.44 -0.1
1,506,691 + clpP Sama_1224 0.53 -0.3
1,506,691 + clpP Sama_1224 0.53 -0.3
1,506,859 - clpP Sama_1224 0.80 -1.7
1,506,944 + +0.0
1,507,023 + -1.3
1,507,104 + -0.9

Or see this region's nucleotide sequence