Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0486 and Psest_0487 are separated by 183 nucleotides Psest_0487 and Psest_0488 are separated by 104 nucleotides
Psest_0486: Psest_0486 - DNA topoisomerase IV, A subunit, proteobacterial, at 535,982 to 538,234
_0486
Psest_0487: Psest_0487 - Uncharacterized protein conserved in bacteria, at 538,418 to 539,125
_0487
Psest_0488: Psest_0488 - Uncharacterized membrane protein affecting hemolysin expression, at 539,230 to 540,762
_0488
Position (kb)
538
539
540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 538.408 kb on + strand at 538.408 kb on + strand at 538.408 kb on + strand at 538.409 kb on - strand at 538.505 kb on + strand, within Psest_0487 at 538.506 kb on - strand, within Psest_0487 at 538.522 kb on + strand, within Psest_0487 at 538.589 kb on + strand, within Psest_0487 at 538.589 kb on + strand, within Psest_0487 at 538.589 kb on + strand, within Psest_0487 at 538.589 kb on + strand, within Psest_0487 at 538.589 kb on + strand, within Psest_0487 at 538.932 kb on + strand, within Psest_0487 at 538.933 kb on - strand, within Psest_0487 at 538.933 kb on - strand, within Psest_0487 at 538.933 kb on - strand, within Psest_0487 at 538.948 kb on - strand, within Psest_0487
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 538,408 + -0.1 538,408 + -0.7 538,408 + -2.3 538,409 - -2.0 538,505 + Psest_0487 0.12 -2.7 538,506 - Psest_0487 0.12 -0.6 538,522 + Psest_0487 0.15 +0.2 538,589 + Psest_0487 0.24 -2.1 538,589 + Psest_0487 0.24 +0.0 538,589 + Psest_0487 0.24 -1.7 538,589 + Psest_0487 0.24 -2.0 538,589 + Psest_0487 0.24 -2.8 538,932 + Psest_0487 0.73 -1.8 538,933 - Psest_0487 0.73 -1.8 538,933 - Psest_0487 0.73 -1.1 538,933 - Psest_0487 0.73 -2.6 538,948 - Psest_0487 0.75 -2.6
Or see this region's nucleotide sequence