Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0032

Experiment: A22 0.005 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	A22 0.005 mg/ml
30,869	+		Psest_0030	0.52	+0.3
30,870	-		Psest_0030	0.52	+0.2
30,974	-		Psest_0030	0.69	+0.7
31,125	-				+0.5
31,125	-				+2.3
31,310	+		Psest_0031	0.43	+1.6
31,311	-		Psest_0031	0.43	+0.0
31,570	+				+0.6
31,570	+				+1.3
31,570	+				-0.9
31,570	+				+0.7
31,571	-				-1.3
31,571	-				-1.5
31,571	-				+2.3
31,663	-				+0.0
31,665	-				+1.3
31,665	-				-0.5
31,680	-				-0.3
31,680	-				-0.5
31,729	-				-1.6
31,747	-				-0.7
31,863	+		Psest_0032	0.14	+0.3
31,864	-		Psest_0032	0.14	+1.0
31,879	+		Psest_0032	0.16	-0.3
31,879	+		Psest_0032	0.16	+0.3
31,879	+		Psest_0032	0.16	-0.4
31,880	-		Psest_0032	0.16	-0.5
31,880	-		Psest_0032	0.16	+0.2
31,880	-		Psest_0032	0.16	+1.6
32,194	+		Psest_0032	0.49	-0.3
32,194	+		Psest_0032	0.49	-0.6
32,194	+		Psest_0032	0.49	-0.5
32,194	+		Psest_0032	0.49	-1.1
32,194	+		Psest_0032	0.49	+0.2
32,194	+		Psest_0032	0.49	+0.3
32,195	-		Psest_0032	0.49	-0.5
32,195	-		Psest_0032	0.49	-1.1
32,195	-		Psest_0032	0.49	+0.3
32,195	-		Psest_0032	0.49	-1.7
32,195	-		Psest_0032	0.49	+0.0
32,197	+		Psest_0032	0.50	-1.8
32,197	+		Psest_0032	0.50	+1.3
32,197	+		Psest_0032	0.50	+2.3
32,197	+		Psest_0032	0.50	+0.5
32,197	+		Psest_0032	0.50	-1.1
32,197	+		Psest_0032	0.50	+1.3
32,197	+		Psest_0032	0.50	-0.5
32,197	+		Psest_0032	0.50	+1.3
32,197	+		Psest_0032	0.50	-3.1
32,198	-		Psest_0032	0.50	-1.1
32,198	-		Psest_0032	0.50	-0.7
32,198	-		Psest_0032	0.50	+0.7
32,198	-		Psest_0032	0.50	-0.5
32,198	-		Psest_0032	0.50	+0.0
32,198	-		Psest_0032	0.50	+0.7
32,198	-		Psest_0032	0.50	-2.7
32,279	-		Psest_0032	0.58	+0.0
32,301	+		Psest_0032	0.61	+0.0
32,341	+		Psest_0032	0.65	+0.5
32,341	+		Psest_0032	0.65	-0.8
32,341	+		Psest_0032	0.65	-0.3
32,341	+		Psest_0032	0.65	-0.7
32,341	+		Psest_0032	0.65	+0.7
32,341	+		Psest_0032	0.65	+0.2
32,342	-		Psest_0032	0.65	-2.5
32,342	-		Psest_0032	0.65	-0.7
32,342	-		Psest_0032	0.65	+1.3
32,342	-		Psest_0032	0.65	+0.0
32,342	-		Psest_0032	0.65	-0.5
32,342	-		Psest_0032	0.65	+0.9
32,342	-		Psest_0032	0.65	-1.5
32,342	-		Psest_0032	0.65	-1.1
32,342	-				+0.7
32,342	-		Psest_0032	0.65	-2.1
32,342	-		Psest_0032	0.65	-0.1
32,454	+		Psest_0032	0.77	-2.1
32,454	+		Psest_0032	0.77	-0.1
32,455	-		Psest_0032	0.77	-0.3
32,455	-		Psest_0032	0.77	+1.7
32,506	+		Psest_0032	0.82	+0.7
32,506	+		Psest_0032	0.82	+0.0
32,506	+		Psest_0032	0.82	-1.7
32,506	+		Psest_0032	0.82	+0.9
32,506	+		Psest_0032	0.82	+0.2
32,506	+		Psest_0032	0.82	-1.3
32,506	+		Psest_0032	0.82	-0.3
32,507	-		Psest_0032	0.83	+0.3
32,507	-		Psest_0032	0.83	-1.2
32,507	-		Psest_0032	0.83	-0.8
32,507	-		Psest_0032	0.83	+0.7
32,507	-		Psest_0032	0.83	+0.7
32,507	-		Psest_0032	0.83	-0.8
32,507	-		Psest_0032	0.83	+0.3
32,514	+		Psest_0032	0.83	+0.3
32,590	+				+0.3
32,591	-				+1.9
32,591	-				+0.2
32,591	-				+0.0
32,736	+				-1.2
32,778	+				+1.1
33,259	+				+1.3
33,259	+				+0.5
33,434	+				-0.5
33,434	+				+3.1
33,435	-				+0.2
33,435	-				+1.3
33,593	+		Psest_0034	0.11	+1.6
33,593	+		Psest_0034	0.11	-0.3
33,594	-		Psest_0034	0.11	+1.6