Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0002 and Psest_0003 are separated by 33 nucleotides Psest_0003 and Psest_0004 are separated by 13 nucleotides
Psest_0002: Psest_0002 - chromosomal replication initiator protein DnaA, at 661 to 2,109
_0002
Psest_0003: Psest_0003 - DNA polymerase III, beta subunit, at 2,143 to 3,246
_0003
Psest_0004: Psest_0004 - recF protein, at 3,260 to 4,372
_0004
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3.245 kb on - strand at 3.258 kb on + strand at 3.258 kb on + strand at 3.258 kb on + strand at 3.258 kb on + strand at 3.258 kb on + strand at 3.259 kb on - strand at 3.259 kb on - strand at 3.316 kb on - strand at 3.332 kb on - strand at 3.446 kb on + strand, within Psest_0004 at 3.446 kb on + strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.447 kb on - strand, within Psest_0004 at 3.540 kb on + strand, within Psest_0004 at 3.540 kb on + strand, within Psest_0004 at 3.540 kb on + strand, within Psest_0004 at 3.541 kb on - strand, within Psest_0004 at 3.541 kb on - strand, within Psest_0004 at 3.541 kb on - strand, within Psest_0004 at 3.613 kb on + strand, within Psest_0004 at 3.622 kb on + strand, within Psest_0004 at 3.622 kb on + strand, within Psest_0004 at 3.622 kb on + strand, within Psest_0004 at 3.766 kb on + strand, within Psest_0004 at 3.766 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.848 kb on + strand, within Psest_0004 at 3.849 kb on - strand, within Psest_0004 at 3.923 kb on + strand, within Psest_0004 at 3.923 kb on + strand, within Psest_0004 at 3.923 kb on + strand, within Psest_0004 at 4.004 kb on - strand, within Psest_0004 at 4.107 kb on + strand, within Psest_0004 at 4.107 kb on + strand, within Psest_0004 at 4.107 kb on + strand, within Psest_0004 at 4.111 kb on + strand, within Psest_0004 at 4.111 kb on + strand, within Psest_0004 at 4.111 kb on + strand, within Psest_0004 at 4.111 kb on + strand, within Psest_0004 at 4.111 kb on + strand, within Psest_0004 at 4.112 kb on - strand, within Psest_0004 at 4.160 kb on + strand, within Psest_0004 at 4.160 kb on + strand, within Psest_0004
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 3,245 - -0.8 3,258 + +0.3 3,258 + -0.7 3,258 + -0.2 3,258 + +0.1 3,258 + -0.4 3,259 - -0.2 3,259 - -3.1 3,316 - -0.8 3,332 - -0.4 3,446 + Psest_0004 0.17 -0.8 3,446 + Psest_0004 0.17 -1.3 3,447 - Psest_0004 0.17 -1.2 3,447 - Psest_0004 0.17 +0.2 3,447 - Psest_0004 0.17 -0.2 3,447 - Psest_0004 0.17 +0.8 3,447 - Psest_0004 0.17 -1.5 3,447 - Psest_0004 0.17 +0.2 3,540 + Psest_0004 0.25 -0.6 3,540 + Psest_0004 0.25 -0.3 3,540 + Psest_0004 0.25 -1.0 3,541 - Psest_0004 0.25 -2.3 3,541 - Psest_0004 0.25 -1.2 3,541 - Psest_0004 0.25 -1.5 3,613 + Psest_0004 0.32 -0.6 3,622 + Psest_0004 0.33 -1.5 3,622 + Psest_0004 0.33 -0.3 3,622 + Psest_0004 0.33 -0.3 3,766 + Psest_0004 0.45 -1.8 3,766 + Psest_0004 0.45 -2.2 3,848 + Psest_0004 0.53 -0.4 3,848 + Psest_0004 0.53 +0.1 3,848 + Psest_0004 0.53 -2.4 3,848 + Psest_0004 0.53 +0.5 3,848 + Psest_0004 0.53 +0.1 3,848 + Psest_0004 0.53 -0.7 3,848 + Psest_0004 0.53 -0.1 3,848 + Psest_0004 0.53 -0.4 3,849 - Psest_0004 0.53 -0.7 3,923 + Psest_0004 0.60 -0.9 3,923 + Psest_0004 0.60 -0.8 3,923 + Psest_0004 0.60 -0.2 4,004 - Psest_0004 0.67 -0.8 4,107 + Psest_0004 0.76 -1.0 4,107 + Psest_0004 0.76 -0.0 4,107 + Psest_0004 0.76 -0.3 4,111 + Psest_0004 0.76 -0.3 4,111 + Psest_0004 0.76 +0.1 4,111 + Psest_0004 0.76 -0.7 4,111 + Psest_0004 0.76 +1.0 4,111 + Psest_0004 0.76 -1.2 4,112 - Psest_0004 0.77 -1.4 4,160 + Psest_0004 0.81 -0.8 4,160 + Psest_0004 0.81 -1.1
Or see this region's nucleotide sequence