Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00950 and PGA1_c00960 overlap by 4 nucleotides PGA1_c00960 and PGA1_c00970 are separated by 121 nucleotides PGA1_c00970 and PGA1_c00980 are separated by 190 nucleotides PGA1_c00980 and PGA1_c00990 are separated by 139 nucleotides
PGA1_c00950: PGA1_c00950 - Protein of unknown function (DUF3429)., at 90,668 to 91,120
_c00950
PGA1_c00960: PGA1_c00960 - lysE type translocator-like protein, at 91,117 to 91,734
_c00960
PGA1_c00970: PGA1_c00970 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase IspH, at 91,856 to 92,806
_c00970
PGA1_c00980: PGA1_c00980 - Uncharacterized conserved protein, at 92,997 to 93,566
_c00980
PGA1_c00990: PGA1_c00990 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase FolK, at 93,706 to 94,275
_c00990
Position (kb) 91
92
93 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 90.878 kb on + strand, within PGA1_c00950 at 90.878 kb on + strand, within PGA1_c00950 at 90.879 kb on + strand, within PGA1_c00950 at 90.963 kb on + strand, within PGA1_c00950 at 90.971 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.032 kb on + strand, within PGA1_c00950 at 91.040 kb on - strand, within PGA1_c00950 at 91.051 kb on + strand, within PGA1_c00950 at 91.056 kb on + strand, within PGA1_c00950 at 91.077 kb on - strand at 91.077 kb on - strand at 91.079 kb on + strand at 91.123 kb on - strand at 91.238 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.284 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.402 kb on - strand, within PGA1_c00960 at 91.523 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.538 kb on + strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.632 kb on - strand, within PGA1_c00960 at 91.639 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.656 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.677 kb on - strand at 91.693 kb on + strand at 91.719 kb on - strand at 91.758 kb on + strand at 91.758 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.794 kb on + strand at 91.820 kb on + strand at 91.830 kb on - strand at 91.830 kb on - strand at 91.847 kb on - strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.856 kb on + strand at 92.859 kb on - strand at 92.859 kb on - strand at 92.859 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.877 kb on + strand at 92.947 kb on + strand at 92.951 kb on + strand at 92.959 kb on - strand at 93.055 kb on + strand, within PGA1_c00980 at 93.069 kb on - strand, within PGA1_c00980 at 93.141 kb on + strand, within PGA1_c00980 at 93.149 kb on + strand, within PGA1_c00980 at 93.157 kb on - strand, within PGA1_c00980 at 93.164 kb on + strand, within PGA1_c00980 at 93.248 kb on + strand, within PGA1_c00980 at 93.255 kb on + strand, within PGA1_c00980 at 93.256 kb on - strand, within PGA1_c00980 at 93.256 kb on - strand, within PGA1_c00980 at 93.285 kb on + strand, within PGA1_c00980 at 93.285 kb on + strand, within PGA1_c00980 at 93.324 kb on - strand, within PGA1_c00980 at 93.341 kb on + strand, within PGA1_c00980 at 93.345 kb on + strand, within PGA1_c00980 at 93.362 kb on - strand, within PGA1_c00980 at 93.413 kb on + strand, within PGA1_c00980 at 93.418 kb on + strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.422 kb on + strand, within PGA1_c00980 at 93.426 kb on - strand, within PGA1_c00980 at 93.430 kb on - strand, within PGA1_c00980 at 93.439 kb on + strand, within PGA1_c00980 at 93.466 kb on - strand, within PGA1_c00980 at 93.494 kb on - strand, within PGA1_c00980 at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.518 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.539 kb on + strand at 93.547 kb on + strand at 93.547 kb on - strand at 93.547 kb on - strand at 93.590 kb on + strand at 93.597 kb on + strand at 93.598 kb on - strand at 93.608 kb on - strand at 93.643 kb on + strand at 93.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 90,878 + PGA1_c00950 0.46 -1.4 90,878 + PGA1_c00950 0.46 +0.6 90,879 + PGA1_c00950 0.47 -1.3 90,963 + PGA1_c00950 0.65 -0.9 90,971 - PGA1_c00950 0.67 -1.1 91,025 - PGA1_c00950 0.79 -0.4 91,025 - PGA1_c00950 0.79 +0.0 91,032 + PGA1_c00950 0.80 -0.9 91,040 - PGA1_c00950 0.82 +0.7 91,051 + PGA1_c00950 0.85 -2.3 91,056 + PGA1_c00950 0.86 +1.3 91,077 - +0.1 91,077 - +1.8 91,079 + +1.3 91,123 - -0.8 91,238 + PGA1_c00960 0.20 +0.1 91,264 + PGA1_c00960 0.24 -0.9 91,264 + PGA1_c00960 0.24 +0.1 91,264 + PGA1_c00960 0.24 -0.7 91,264 + PGA1_c00960 0.24 +0.0 91,264 + PGA1_c00960 0.24 -1.4 91,272 - PGA1_c00960 0.25 -0.0 91,272 - PGA1_c00960 0.25 +0.7 91,272 - PGA1_c00960 0.25 -0.8 91,272 - PGA1_c00960 0.25 -0.3 91,272 - PGA1_c00960 0.25 -2.4 91,272 - PGA1_c00960 0.25 -0.6 91,272 - PGA1_c00960 0.25 -0.8 91,272 - PGA1_c00960 0.25 +1.4 91,272 - PGA1_c00960 0.25 +0.9 91,272 - PGA1_c00960 0.25 +0.2 91,284 + PGA1_c00960 0.27 +0.9 91,368 + PGA1_c00960 0.41 -0.5 91,368 + PGA1_c00960 0.41 +0.7 91,402 - PGA1_c00960 0.46 +3.1 91,523 + PGA1_c00960 0.66 +1.1 91,536 + PGA1_c00960 0.68 +0.5 91,536 + PGA1_c00960 0.68 +0.7 91,536 + PGA1_c00960 0.68 +0.0 91,536 + PGA1_c00960 0.68 +1.7 91,536 + PGA1_c00960 0.68 -2.8 91,536 + PGA1_c00960 0.68 +0.6 91,536 + PGA1_c00960 0.68 -0.7 91,536 + PGA1_c00960 0.68 +1.1 91,536 + PGA1_c00960 0.68 -0.5 91,538 + PGA1_c00960 0.68 -0.3 91,544 - PGA1_c00960 0.69 +0.2 91,544 - PGA1_c00960 0.69 -0.4 91,544 - PGA1_c00960 0.69 +1.3 91,544 - PGA1_c00960 0.69 +0.1 91,544 - PGA1_c00960 0.69 +1.1 91,551 - PGA1_c00960 0.70 -1.0 91,551 - PGA1_c00960 0.70 +1.1 91,554 - PGA1_c00960 0.71 -0.1 91,554 - PGA1_c00960 0.71 -0.3 91,632 - PGA1_c00960 0.83 -0.6 91,639 + PGA1_c00960 0.84 -0.9 91,644 + PGA1_c00960 0.85 +0.2 91,644 + PGA1_c00960 0.85 +1.1 91,656 + PGA1_c00960 0.87 -0.9 91,664 + PGA1_c00960 0.89 -0.3 91,664 + PGA1_c00960 0.89 -1.7 91,674 + -1.0 91,674 + -0.6 91,674 + +0.4 91,674 + -1.5 91,677 - -0.5 91,693 + +0.6 91,719 - +1.5 91,758 + -0.0 91,758 + -1.2 91,768 + -0.7 91,768 + -4.1 91,768 + +0.1 91,768 + -2.8 91,768 + -0.2 91,768 + +0.7 91,768 + -0.2 91,768 + +0.9 91,776 - -0.1 91,776 - -0.2 91,776 - +0.3 91,776 - -2.2 91,776 - -0.4 91,776 - -2.2 91,776 - +1.7 91,776 - +1.2 91,776 - -2.9 91,776 - -2.4 91,776 - -1.9 91,776 - +1.6 91,776 - +1.4 91,776 - -0.4 91,776 - -0.0 91,794 + +0.4 91,820 + +1.6 91,830 - +0.8 91,830 - +0.7 91,847 - +0.6 92,851 + +0.3 92,851 + -0.2 92,851 + -0.1 92,851 + +0.4 92,851 + -0.1 92,856 + +1.1 92,859 - +0.0 92,859 - +1.5 92,859 - -0.7 92,864 - -0.9 92,864 - -0.2 92,864 - -0.7 92,864 - -0.6 92,864 - -2.0 92,877 + +1.1 92,947 + -0.7 92,951 + -2.7 92,959 - -1.4 93,055 + PGA1_c00980 0.10 -1.4 93,069 - PGA1_c00980 0.13 -0.4 93,141 + PGA1_c00980 0.25 +0.3 93,149 + PGA1_c00980 0.27 -0.9 93,157 - PGA1_c00980 0.28 -2.8 93,164 + PGA1_c00980 0.29 -3.8 93,248 + PGA1_c00980 0.44 -0.3 93,255 + PGA1_c00980 0.45 -1.8 93,256 - PGA1_c00980 0.45 -0.4 93,256 - PGA1_c00980 0.45 -0.3 93,285 + PGA1_c00980 0.51 +0.3 93,285 + PGA1_c00980 0.51 -1.3 93,324 - PGA1_c00980 0.57 -3.2 93,341 + PGA1_c00980 0.60 -3.1 93,345 + PGA1_c00980 0.61 -1.5 93,362 - PGA1_c00980 0.64 -1.3 93,413 + PGA1_c00980 0.73 -0.9 93,418 + PGA1_c00980 0.74 -1.0 93,421 - PGA1_c00980 0.74 -1.3 93,421 - PGA1_c00980 0.74 -1.0 93,422 + PGA1_c00980 0.75 -1.0 93,426 - PGA1_c00980 0.75 -0.6 93,430 - PGA1_c00980 0.76 +0.1 93,439 + PGA1_c00980 0.78 -0.6 93,466 - PGA1_c00980 0.82 -1.4 93,494 - PGA1_c00980 0.87 -1.1 93,511 + -2.0 93,511 + -1.7 93,511 + -1.8 93,511 + -3.3 93,511 + -1.2 93,518 + -2.7 93,529 + -1.7 93,529 + -2.3 93,539 + -0.3 93,547 + -0.9 93,547 - -1.3 93,547 - -2.3 93,590 + -1.0 93,597 + -3.5 93,598 - -1.5 93,608 - +0.3 93,643 + -1.3 93,652 - -0.9
Or see this region's nucleotide sequence