Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08960 and PGA1_c08970 are separated by 19 nucleotides PGA1_c08970 and PGA1_c08980 are separated by 205 nucleotides PGA1_c08980 and PGA1_c08990 are separated by 142 nucleotides PGA1_c08990 and PGA1_c09000 overlap by 2 nucleotides PGA1_c09000 and PGA1_c09010 are separated by 220 nucleotides
PGA1_c08960: PGA1_c08960 - DNA repair endonuclease mmcB (DUF1052) (from data) , at 925,190 to 925,645
_c08960
PGA1_c08970: PGA1_c08970 - hypothetical protein, at 925,665 to 925,853
_c08970
PGA1_c08980: PGA1_c08980 - tRNA-His, at 926,059 to 926,135
_c08980
PGA1_c08990: PGA1_c08990 - hypothetical protein, at 926,278 to 926,592
_c08990
PGA1_c09000: PGA1_c09000 - ribosomal large subunit pseudouridine synthase D, at 926,591 to 927,625
_c09000
PGA1_c09010: PGA1_c09010 - RNA polymerase sigma-32 factor RpoH, at 927,846 to 928,742
_c09010
Position (kb)
926
927
928 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 925.625 kb on + strand at 925.632 kb on - strand at 925.633 kb on - strand at 925.699 kb on + strand, within PGA1_c08970 at 925.840 kb on + strand at 925.873 kb on - strand at 925.880 kb on - strand at 925.901 kb on + strand at 925.929 kb on + strand at 925.944 kb on + strand at 925.952 kb on - strand at 925.952 kb on - strand at 925.963 kb on - strand at 925.963 kb on - strand at 926.233 kb on + strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.243 kb on - strand at 926.293 kb on - strand at 926.293 kb on - strand at 926.340 kb on - strand, within PGA1_c08990 at 926.439 kb on + strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.484 kb on - strand, within PGA1_c08990 at 926.596 kb on - strand at 926.692 kb on + strand at 926.971 kb on + strand, within PGA1_c09000 at 926.989 kb on + strand, within PGA1_c09000 at 927.011 kb on - strand, within PGA1_c09000 at 927.030 kb on + strand at 927.038 kb on - strand, within PGA1_c09000 at 927.068 kb on + strand, within PGA1_c09000 at 927.129 kb on + strand, within PGA1_c09000 at 927.137 kb on - strand, within PGA1_c09000 at 927.270 kb on - strand, within PGA1_c09000 at 927.270 kb on - strand, within PGA1_c09000 at 927.337 kb on + strand, within PGA1_c09000 at 927.366 kb on + strand, within PGA1_c09000 at 927.405 kb on + strand, within PGA1_c09000 at 927.406 kb on - strand, within PGA1_c09000 at 927.528 kb on + strand at 927.547 kb on - strand at 927.729 kb on - strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.773 kb on + strand at 927.781 kb on - strand at 927.781 kb on - strand at 927.783 kb on + strand at 927.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 925,625 + -1.3 925,632 - -0.7 925,633 - -2.5 925,699 + PGA1_c08970 0.18 -0.7 925,840 + -3.2 925,873 - -1.4 925,880 - -1.1 925,901 + -1.3 925,929 + +1.9 925,944 + +0.2 925,952 - -1.7 925,952 - +0.3 925,963 - +0.7 925,963 - -0.3 926,233 + -0.1 926,243 - -0.9 926,243 - -0.1 926,243 - -0.3 926,243 - -0.7 926,243 - +0.1 926,243 - -0.3 926,243 - +0.3 926,293 - +0.8 926,293 - +0.8 926,340 - PGA1_c08990 0.20 -1.2 926,439 + PGA1_c08990 0.51 -1.8 926,484 - PGA1_c08990 0.65 -3.1 926,484 - PGA1_c08990 0.65 -1.6 926,596 - +4.0 926,692 + +3.6 926,971 + PGA1_c09000 0.37 +4.1 926,989 + PGA1_c09000 0.38 +3.2 927,011 - PGA1_c09000 0.41 +3.9 927,030 + +1.9 927,038 - PGA1_c09000 0.43 +4.9 927,068 + PGA1_c09000 0.46 +3.8 927,129 + PGA1_c09000 0.52 +4.6 927,137 - PGA1_c09000 0.53 +6.2 927,270 - PGA1_c09000 0.66 +2.7 927,270 - PGA1_c09000 0.66 +5.1 927,337 + PGA1_c09000 0.72 +3.8 927,366 + PGA1_c09000 0.75 +4.3 927,405 + PGA1_c09000 0.79 +4.8 927,406 - PGA1_c09000 0.79 +4.6 927,528 + +4.6 927,547 - +4.5 927,729 - -1.1 927,773 + -0.8 927,773 + -0.7 927,773 + -1.6 927,773 + -1.7 927,773 + -0.3 927,781 - -1.1 927,781 - +1.2 927,783 + -2.0 927,785 + -1.6
Or see this region's nucleotide sequence