Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05040 and PGA1_c05050 are separated by 311 nucleotides PGA1_c05050 and PGA1_c05060 are separated by 124 nucleotides PGA1_c05060 and PGA1_c05070 are separated by 20 nucleotides
PGA1_c05040: PGA1_c05040 - transposase, at 498,177 to 498,566
_c05040
PGA1_c05050: PGA1_c05050 - tRNA-Ser, at 498,878 to 498,967
_c05050
PGA1_c05060: PGA1_c05060 - putative short chain dehydrogenase, at 499,092 to 499,790
_c05060
PGA1_c05070: PGA1_c05070 - putative pyridoxal-phosphate-dependent aminotransferase, at 499,811 to 500,908
_c05070
Position (kb)
499
500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 498.635 kb on - strand at 498.659 kb on + strand at 498.680 kb on + strand at 498.693 kb on - strand at 498.718 kb on - strand at 498.740 kb on + strand at 498.740 kb on + strand at 498.740 kb on + strand at 498.740 kb on + strand at 498.748 kb on - strand at 498.748 kb on - strand at 498.748 kb on - strand at 498.755 kb on + strand at 498.986 kb on + strand at 499.004 kb on - strand at 499.015 kb on + strand at 499.066 kb on - strand at 499.078 kb on + strand at 499.113 kb on + strand at 499.121 kb on - strand at 499.123 kb on + strand at 499.123 kb on + strand at 499.123 kb on + strand at 499.123 kb on + strand at 499.123 kb on + strand at 499.124 kb on + strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.151 kb on + strand at 499.175 kb on - strand, within PGA1_c05060 at 499.197 kb on + strand, within PGA1_c05060 at 499.197 kb on + strand, within PGA1_c05060 at 499.205 kb on - strand, within PGA1_c05060 at 499.210 kb on - strand, within PGA1_c05060 at 499.262 kb on - strand, within PGA1_c05060 at 499.262 kb on - strand, within PGA1_c05060 at 499.263 kb on + strand, within PGA1_c05060 at 499.276 kb on - strand, within PGA1_c05060 at 499.280 kb on + strand, within PGA1_c05060 at 499.280 kb on + strand, within PGA1_c05060 at 499.280 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.289 kb on + strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.297 kb on - strand, within PGA1_c05060 at 499.306 kb on + strand, within PGA1_c05060 at 499.308 kb on + strand, within PGA1_c05060 at 499.352 kb on + strand, within PGA1_c05060 at 499.407 kb on - strand, within PGA1_c05060 at 499.449 kb on + strand, within PGA1_c05060 at 499.524 kb on + strand, within PGA1_c05060 at 499.524 kb on + strand, within PGA1_c05060 at 499.524 kb on + strand, within PGA1_c05060 at 499.530 kb on + strand, within PGA1_c05060 at 499.532 kb on - strand, within PGA1_c05060 at 499.545 kb on + strand, within PGA1_c05060 at 499.545 kb on + strand, within PGA1_c05060 at 499.545 kb on + strand, within PGA1_c05060 at 499.550 kb on + strand, within PGA1_c05060 at 499.550 kb on + strand, within PGA1_c05060 at 499.550 kb on + strand, within PGA1_c05060 at 499.550 kb on + strand, within PGA1_c05060 at 499.553 kb on - strand, within PGA1_c05060 at 499.558 kb on - strand, within PGA1_c05060 at 499.558 kb on - strand, within PGA1_c05060 at 499.558 kb on - strand, within PGA1_c05060 at 499.575 kb on + strand, within PGA1_c05060 at 499.610 kb on + strand, within PGA1_c05060 at 499.610 kb on + strand, within PGA1_c05060 at 499.654 kb on - strand, within PGA1_c05060 at 499.665 kb on + strand, within PGA1_c05060 at 499.683 kb on - strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.685 kb on + strand, within PGA1_c05060 at 499.690 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.693 kb on - strand, within PGA1_c05060 at 499.730 kb on + strand at 499.738 kb on + strand at 499.738 kb on + strand at 499.738 kb on + strand at 499.738 kb on + strand at 499.738 kb on + strand at 499.746 kb on - strand at 499.783 kb on + strand at 499.801 kb on - strand at 499.809 kb on + strand at 499.830 kb on - strand at 499.865 kb on - strand at 499.867 kb on - strand at 499.867 kb on - strand at 499.867 kb on - strand at 499.872 kb on - strand at 499.972 kb on + strand, within PGA1_c05070 at 499.977 kb on + strand, within PGA1_c05070 at 499.977 kb on + strand, within PGA1_c05070 at 499.982 kb on + strand, within PGA1_c05070 at 499.985 kb on - strand, within PGA1_c05070 at 500.040 kb on + strand, within PGA1_c05070 at 500.104 kb on + strand, within PGA1_c05070 at 500.114 kb on + strand, within PGA1_c05070 at 500.176 kb on - strand, within PGA1_c05070 at 500.211 kb on + strand, within PGA1_c05070 at 500.225 kb on + strand, within PGA1_c05070 at 500.225 kb on + strand, within PGA1_c05070 at 500.293 kb on + strand, within PGA1_c05070 at 500.293 kb on + strand, within PGA1_c05070 at 500.359 kb on + strand, within PGA1_c05070 at 500.367 kb on - strand, within PGA1_c05070 at 500.449 kb on + strand, within PGA1_c05070 at 500.449 kb on + strand, within PGA1_c05070 at 500.457 kb on - strand, within PGA1_c05070 at 500.520 kb on + strand, within PGA1_c05070 at 500.543 kb on + strand, within PGA1_c05070 at 500.589 kb on + strand, within PGA1_c05070 at 500.611 kb on + strand, within PGA1_c05070 at 500.622 kb on + strand, within PGA1_c05070 at 500.631 kb on + strand, within PGA1_c05070 at 500.640 kb on - strand, within PGA1_c05070 at 500.684 kb on - strand, within PGA1_c05070 at 500.713 kb on + strand, within PGA1_c05070 at 500.740 kb on + strand, within PGA1_c05070 at 500.748 kb on - strand, within PGA1_c05070 at 500.748 kb on - strand, within PGA1_c05070 at 500.750 kb on + strand, within PGA1_c05070 at 500.787 kb on + strand, within PGA1_c05070
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 498,635 - -2.6 498,659 + -0.2 498,680 + -1.8 498,693 - -0.3 498,718 - +0.2 498,740 + -0.7 498,740 + -0.3 498,740 + +0.1 498,740 + -1.4 498,748 - -1.3 498,748 - +0.4 498,748 - -0.2 498,755 + -0.0 498,986 + +1.9 499,004 - +0.6 499,015 + +1.4 499,066 - +2.0 499,078 + +0.4 499,113 + +0.6 499,121 - -1.6 499,123 + -0.8 499,123 + -0.5 499,123 + -3.2 499,123 + -0.2 499,123 + -0.2 499,124 + -0.6 499,131 - -1.5 499,131 - -0.8 499,131 - -0.5 499,131 - +0.4 499,131 - +0.6 499,131 - +0.5 499,131 - +0.2 499,131 - +1.5 499,131 - +2.2 499,131 - +0.6 499,151 + -2.5 499,175 - PGA1_c05060 0.12 -1.9 499,197 + PGA1_c05060 0.15 +0.1 499,197 + PGA1_c05060 0.15 -0.7 499,205 - PGA1_c05060 0.16 +0.2 499,210 - PGA1_c05060 0.17 +0.6 499,262 - PGA1_c05060 0.24 -1.5 499,262 - PGA1_c05060 0.24 -1.5 499,263 + PGA1_c05060 0.24 -0.6 499,276 - PGA1_c05060 0.26 -0.6 499,280 + PGA1_c05060 0.27 +1.3 499,280 + PGA1_c05060 0.27 +0.2 499,280 + PGA1_c05060 0.27 -0.6 499,289 + PGA1_c05060 0.28 +0.9 499,289 + PGA1_c05060 0.28 +0.3 499,289 + PGA1_c05060 0.28 -0.4 499,289 + PGA1_c05060 0.28 -1.9 499,289 + PGA1_c05060 0.28 +0.4 499,289 + PGA1_c05060 0.28 +0.5 499,289 + PGA1_c05060 0.28 -0.5 499,289 + PGA1_c05060 0.28 -1.1 499,289 + PGA1_c05060 0.28 +0.5 499,289 + PGA1_c05060 0.28 -1.8 499,289 + PGA1_c05060 0.28 -2.4 499,289 + PGA1_c05060 0.28 -0.5 499,289 + PGA1_c05060 0.28 +0.2 499,289 + PGA1_c05060 0.28 +0.3 499,289 + PGA1_c05060 0.28 +0.5 499,289 + PGA1_c05060 0.28 +0.7 499,289 + PGA1_c05060 0.28 +0.5 499,289 + PGA1_c05060 0.28 +0.6 499,289 + PGA1_c05060 0.28 +2.0 499,289 + PGA1_c05060 0.28 +0.1 499,289 + PGA1_c05060 0.28 +1.4 499,289 + PGA1_c05060 0.28 -0.4 499,289 + PGA1_c05060 0.28 -0.8 499,289 + PGA1_c05060 0.28 -0.5 499,289 + PGA1_c05060 0.28 -1.3 499,289 + PGA1_c05060 0.28 -0.9 499,289 + PGA1_c05060 0.28 +1.5 499,297 - PGA1_c05060 0.29 +0.2 499,297 - PGA1_c05060 0.29 -0.9 499,297 - PGA1_c05060 0.29 +0.3 499,297 - PGA1_c05060 0.29 -0.9 499,297 - PGA1_c05060 0.29 -1.6 499,297 - PGA1_c05060 0.29 +0.6 499,297 - PGA1_c05060 0.29 -1.2 499,297 - PGA1_c05060 0.29 -0.4 499,297 - PGA1_c05060 0.29 +0.2 499,297 - PGA1_c05060 0.29 -0.2 499,297 - PGA1_c05060 0.29 +0.6 499,297 - PGA1_c05060 0.29 -0.4 499,297 - PGA1_c05060 0.29 +0.2 499,297 - PGA1_c05060 0.29 -0.4 499,297 - PGA1_c05060 0.29 -1.4 499,306 + PGA1_c05060 0.31 +2.8 499,308 + PGA1_c05060 0.31 +1.3 499,352 + PGA1_c05060 0.37 -1.2 499,407 - PGA1_c05060 0.45 -0.9 499,449 + PGA1_c05060 0.51 +0.0 499,524 + PGA1_c05060 0.62 -1.2 499,524 + PGA1_c05060 0.62 +0.6 499,524 + PGA1_c05060 0.62 -0.1 499,530 + PGA1_c05060 0.63 +0.5 499,532 - PGA1_c05060 0.63 -0.7 499,545 + PGA1_c05060 0.65 +0.1 499,545 + PGA1_c05060 0.65 +0.2 499,545 + PGA1_c05060 0.65 +1.9 499,550 + PGA1_c05060 0.66 +0.7 499,550 + PGA1_c05060 0.66 -0.8 499,550 + PGA1_c05060 0.66 +0.9 499,550 + PGA1_c05060 0.66 -1.2 499,553 - PGA1_c05060 0.66 +1.4 499,558 - PGA1_c05060 0.67 -3.6 499,558 - PGA1_c05060 0.67 +0.4 499,558 - PGA1_c05060 0.67 -0.9 499,575 + PGA1_c05060 0.69 -2.6 499,610 + PGA1_c05060 0.74 -1.5 499,610 + PGA1_c05060 0.74 +0.7 499,654 - PGA1_c05060 0.80 +0.6 499,665 + PGA1_c05060 0.82 -1.0 499,683 - PGA1_c05060 0.85 -0.2 499,685 + PGA1_c05060 0.85 -0.5 499,685 + PGA1_c05060 0.85 -1.3 499,685 + PGA1_c05060 0.85 +1.4 499,685 + PGA1_c05060 0.85 +0.9 499,685 + PGA1_c05060 0.85 -0.3 499,685 + PGA1_c05060 0.85 +0.0 499,685 + PGA1_c05060 0.85 -0.0 499,685 + PGA1_c05060 0.85 +0.5 499,685 + PGA1_c05060 0.85 -0.1 499,685 + PGA1_c05060 0.85 +0.2 499,685 + PGA1_c05060 0.85 -3.4 499,685 + PGA1_c05060 0.85 +0.3 499,685 + PGA1_c05060 0.85 -0.6 499,685 + PGA1_c05060 0.85 -1.4 499,685 + PGA1_c05060 0.85 -2.4 499,685 + PGA1_c05060 0.85 -0.6 499,685 + PGA1_c05060 0.85 +0.1 499,685 + PGA1_c05060 0.85 -0.6 499,685 + PGA1_c05060 0.85 -2.6 499,685 + PGA1_c05060 0.85 -0.4 499,690 - PGA1_c05060 0.86 -2.4 499,693 - PGA1_c05060 0.86 +1.4 499,693 - PGA1_c05060 0.86 -1.8 499,693 - PGA1_c05060 0.86 -2.0 499,693 - PGA1_c05060 0.86 +1.7 499,693 - PGA1_c05060 0.86 -0.8 499,693 - PGA1_c05060 0.86 +1.2 499,693 - PGA1_c05060 0.86 -0.6 499,693 - PGA1_c05060 0.86 +0.3 499,693 - PGA1_c05060 0.86 +0.2 499,693 - PGA1_c05060 0.86 +0.7 499,693 - PGA1_c05060 0.86 +0.9 499,730 + +0.6 499,738 + -0.5 499,738 + -2.1 499,738 + -0.4 499,738 + +0.8 499,738 + -0.3 499,746 - +2.3 499,783 + -0.0 499,801 - -2.4 499,809 + -0.6 499,830 - +0.2 499,865 - -0.3 499,867 - -0.2 499,867 - +1.3 499,867 - +1.2 499,872 - -1.4 499,972 + PGA1_c05070 0.15 -3.4 499,977 + PGA1_c05070 0.15 -2.2 499,977 + PGA1_c05070 0.15 -1.0 499,982 + PGA1_c05070 0.16 +0.1 499,985 - PGA1_c05070 0.16 -3.0 500,040 + PGA1_c05070 0.21 -1.3 500,104 + PGA1_c05070 0.27 -0.3 500,114 + PGA1_c05070 0.28 -0.6 500,176 - PGA1_c05070 0.33 -0.2 500,211 + PGA1_c05070 0.36 -0.3 500,225 + PGA1_c05070 0.38 -1.3 500,225 + PGA1_c05070 0.38 +1.2 500,293 + PGA1_c05070 0.44 +0.6 500,293 + PGA1_c05070 0.44 -0.2 500,359 + PGA1_c05070 0.50 -1.5 500,367 - PGA1_c05070 0.51 -0.3 500,449 + PGA1_c05070 0.58 -0.9 500,449 + PGA1_c05070 0.58 -1.2 500,457 - PGA1_c05070 0.59 -1.2 500,520 + PGA1_c05070 0.65 -0.1 500,543 + PGA1_c05070 0.67 -1.0 500,589 + PGA1_c05070 0.71 -1.5 500,611 + PGA1_c05070 0.73 +1.2 500,622 + PGA1_c05070 0.74 -2.7 500,631 + PGA1_c05070 0.75 -0.6 500,640 - PGA1_c05070 0.76 +0.3 500,684 - PGA1_c05070 0.80 -0.4 500,713 + PGA1_c05070 0.82 -2.0 500,740 + PGA1_c05070 0.85 -0.8 500,748 - PGA1_c05070 0.85 -1.2 500,748 - PGA1_c05070 0.85 -0.7 500,750 + PGA1_c05070 0.86 -1.3 500,787 + PGA1_c05070 0.89 +1.9
Or see this region's nucleotide sequence