Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03730 and PGA1_c03740 are separated by 192 nucleotides PGA1_c03740 and PGA1_c03750 are separated by 18 nucleotides PGA1_c03750 and PGA1_c03760 are separated by 15 nucleotides PGA1_c03760 and PGA1_c03770 are separated by 84 nucleotides
PGA1_c03730: PGA1_c03730 - Protein of unknown function (DUF1194)., at 358,494 to 359,183
_c03730
PGA1_c03740: PGA1_c03740 - succinate dehydrogenase cytochrome b556 subunit, at 359,376 to 359,759
_c03740
PGA1_c03750: PGA1_c03750 - succinate dehydrogenase hydrophobic membrane anchor subunit, at 359,778 to 360,149
_c03750
PGA1_c03760: PGA1_c03760 - succinate dehydrogenase flavoprotein subunit, at 360,165 to 361,970
_c03760
PGA1_c03770: PGA1_c03770 - hypothetical protein, at 362,055 to 362,969
_c03770
Position (kb) 360
361
362 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 359.169 kb on - strand at 359.169 kb on - strand at 359.169 kb on - strand at 359.203 kb on - strand at 359.758 kb on + strand at 360.126 kb on + strand at 361.972 kb on + strand at 362.040 kb on + strand at 362.042 kb on + strand at 362.042 kb on + strand at 362.494 kb on + strand, within PGA1_c03770 at 362.516 kb on + strand, within PGA1_c03770 at 362.516 kb on + strand, within PGA1_c03770 at 362.630 kb on + strand, within PGA1_c03770 at 362.735 kb on + strand, within PGA1_c03770 at 362.737 kb on - strand, within PGA1_c03770 at 362.754 kb on + strand, within PGA1_c03770 at 362.766 kb on + strand, within PGA1_c03770 at 362.766 kb on + strand, within PGA1_c03770 at 362.852 kb on - strand, within PGA1_c03770 at 362.884 kb on - strand at 362.899 kb on + strand at 362.963 kb on + strand at 362.963 kb on + strand at 362.963 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 359,169 - +1.4 359,169 - -0.2 359,169 - -0.4 359,203 - +1.2 359,758 + -0.3 360,126 + -3.4 361,972 + +1.6 362,040 + +0.3 362,042 + +1.7 362,042 + +2.2 362,494 + PGA1_c03770 0.48 -1.0 362,516 + PGA1_c03770 0.50 -1.8 362,516 + PGA1_c03770 0.50 -0.9 362,630 + PGA1_c03770 0.63 +1.6 362,735 + PGA1_c03770 0.74 +0.5 362,737 - PGA1_c03770 0.75 -2.7 362,754 + PGA1_c03770 0.76 +1.8 362,766 + PGA1_c03770 0.78 -0.7 362,766 + PGA1_c03770 0.78 +0.3 362,852 - PGA1_c03770 0.87 -1.5 362,884 - -0.6 362,899 + -0.1 362,963 + +1.0 362,963 + -1.2 362,963 + -1.7
Or see this region's nucleotide sequence