Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35260 and PGA1_c35270 are separated by 334 nucleotides PGA1_c35270 and PGA1_c35280 are separated by 81 nucleotides PGA1_c35280 and PGA1_c35290 overlap by 4 nucleotides PGA1_c35290 and PGA1_c35300 are separated by 92 nucleotides PGA1_c35300 and PGA1_c35310 are separated by 62 nucleotides
PGA1_c35260: PGA1_c35260 - ATP-dependent protease HslV, at 3,697,348 to 3,697,911
_c35260
PGA1_c35270: PGA1_c35270 - Uncharacterized conserved small protein, at 3,698,246 to 3,698,440
_c35270
PGA1_c35280: PGA1_c35280 - conserved hypothetical protein, at 3,698,522 to 3,698,887
_c35280
PGA1_c35290: PGA1_c35290 - Conserved hypothetical protein (Lin0512_fam)., at 3,698,884 to 3,699,231
_c35290
PGA1_c35300: PGA1_c35300 - thioredoxin TrxA, at 3,699,324 to 3,699,644
_c35300
PGA1_c35310: PGA1_c35310 - double strand break repair helicase AddA, at 3,699,707 to 3,703,075
_c35310
Position (kb)
3698
3699
3700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3697.931 kb on - strand at 3697.998 kb on - strand at 3698.076 kb on + strand at 3698.265 kb on + strand at 3698.265 kb on + strand at 3698.319 kb on + strand, within PGA1_c35270 at 3698.385 kb on + strand, within PGA1_c35270 at 3698.393 kb on - strand, within PGA1_c35270 at 3698.442 kb on - strand at 3698.442 kb on - strand at 3698.450 kb on + strand at 3698.453 kb on - strand at 3698.477 kb on + strand at 3698.509 kb on - strand at 3698.541 kb on + strand at 3698.541 kb on + strand at 3698.555 kb on - strand at 3698.572 kb on - strand, within PGA1_c35280 at 3698.630 kb on + strand, within PGA1_c35280 at 3698.683 kb on + strand, within PGA1_c35280 at 3698.748 kb on + strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.754 kb on - strand, within PGA1_c35280 at 3698.795 kb on + strand, within PGA1_c35280 at 3698.803 kb on + strand, within PGA1_c35280 at 3698.803 kb on - strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.817 kb on + strand, within PGA1_c35280 at 3698.822 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.823 kb on + strand, within PGA1_c35280 at 3698.826 kb on - strand, within PGA1_c35280 at 3698.830 kb on - strand, within PGA1_c35280 at 3698.831 kb on - strand, within PGA1_c35280 at 3698.831 kb on - strand, within PGA1_c35280 at 3698.937 kb on - strand, within PGA1_c35290 at 3698.938 kb on + strand, within PGA1_c35290 at 3698.974 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.975 kb on + strand, within PGA1_c35290 at 3698.983 kb on - strand, within PGA1_c35290 at 3698.983 kb on - strand, within PGA1_c35290 at 3699.007 kb on - strand, within PGA1_c35290 at 3699.015 kb on - strand, within PGA1_c35290 at 3699.020 kb on - strand, within PGA1_c35290 at 3699.049 kb on - strand, within PGA1_c35290 at 3699.073 kb on + strand, within PGA1_c35290 at 3699.081 kb on - strand, within PGA1_c35290 at 3699.127 kb on + strand, within PGA1_c35290 at 3699.175 kb on - strand, within PGA1_c35290 at 3699.175 kb on - strand, within PGA1_c35290 at 3699.197 kb on + strand at 3699.227 kb on + strand at 3699.248 kb on - strand at 3699.724 kb on + strand at 3699.747 kb on - strand at 3699.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 3,697,931 - -0.6 3,697,998 - +1.3 3,698,076 + -3.7 3,698,265 + +0.3 3,698,265 + -0.7 3,698,319 + PGA1_c35270 0.37 +1.5 3,698,385 + PGA1_c35270 0.71 +0.1 3,698,393 - PGA1_c35270 0.75 +0.3 3,698,442 - -0.3 3,698,442 - -0.1 3,698,450 + +0.8 3,698,453 - -0.1 3,698,477 + +2.1 3,698,509 - -0.6 3,698,541 + -2.0 3,698,541 + -0.7 3,698,555 - -0.7 3,698,572 - PGA1_c35280 0.14 -0.8 3,698,630 + PGA1_c35280 0.30 +0.4 3,698,683 + PGA1_c35280 0.44 -0.7 3,698,748 + PGA1_c35280 0.62 -0.3 3,698,754 - PGA1_c35280 0.63 +0.2 3,698,754 - PGA1_c35280 0.63 +0.7 3,698,754 - PGA1_c35280 0.63 +0.4 3,698,795 + PGA1_c35280 0.75 -0.4 3,698,803 + PGA1_c35280 0.77 +0.5 3,698,803 - PGA1_c35280 0.77 +0.2 3,698,817 + PGA1_c35280 0.81 +0.5 3,698,817 + PGA1_c35280 0.81 +3.0 3,698,817 + PGA1_c35280 0.81 +0.1 3,698,822 + PGA1_c35280 0.82 -0.0 3,698,823 + PGA1_c35280 0.82 -0.3 3,698,823 + PGA1_c35280 0.82 -0.6 3,698,823 + PGA1_c35280 0.82 +0.3 3,698,826 - PGA1_c35280 0.83 +0.2 3,698,830 - PGA1_c35280 0.84 +1.4 3,698,831 - PGA1_c35280 0.84 -2.9 3,698,831 - PGA1_c35280 0.84 -0.9 3,698,937 - PGA1_c35290 0.15 +0.9 3,698,938 + PGA1_c35290 0.16 -0.3 3,698,974 + PGA1_c35290 0.26 +1.2 3,698,975 + PGA1_c35290 0.26 +0.6 3,698,975 + PGA1_c35290 0.26 -0.4 3,698,975 + PGA1_c35290 0.26 +1.0 3,698,983 - PGA1_c35290 0.28 +0.8 3,698,983 - PGA1_c35290 0.28 +1.4 3,699,007 - PGA1_c35290 0.35 -0.9 3,699,015 - PGA1_c35290 0.38 -1.0 3,699,020 - PGA1_c35290 0.39 -0.2 3,699,049 - PGA1_c35290 0.47 -2.7 3,699,073 + PGA1_c35290 0.54 -1.6 3,699,081 - PGA1_c35290 0.57 -1.0 3,699,127 + PGA1_c35290 0.70 -3.4 3,699,175 - PGA1_c35290 0.84 -0.6 3,699,175 - PGA1_c35290 0.84 +0.1 3,699,197 + +0.6 3,699,227 + +0.9 3,699,248 - -0.5 3,699,724 + -0.1 3,699,747 - +0.2 3,699,751 - +0.9
Or see this region's nucleotide sequence