Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35170 and PGA1_c35180 are separated by 75 nucleotides PGA1_c35180 and PGA1_c35190 are separated by 78 nucleotides PGA1_c35190 and PGA1_c35200 are separated by 115 nucleotides PGA1_c35200 and PGA1_c35210 overlap by 4 nucleotides
PGA1_c35170: PGA1_c35170 - DNA polymerase III subunit epsilon, at 3,689,382 to 3,690,119
_c35170
PGA1_c35180: PGA1_c35180 - protein-export protein SecB, at 3,690,195 to 3,690,689
_c35180
PGA1_c35190: PGA1_c35190 - putative protein FxsA, at 3,690,768 to 3,691,274
_c35190
PGA1_c35200: PGA1_c35200 - putative transporter protein, at 3,691,390 to 3,692,046
_c35200
PGA1_c35210: PGA1_c35210 - putative membrane-bound lytic murein transglycosylase A, at 3,692,043 to 3,693,098
_c35210
Position (kb)
3690
3691
3692 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3689.989 kb on + strand, within PGA1_c35170 at 3690.007 kb on + strand, within PGA1_c35170 at 3690.093 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.098 kb on + strand at 3690.104 kb on - strand at 3690.161 kb on - strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.204 kb on + strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.766 kb on + strand at 3690.771 kb on + strand at 3690.779 kb on + strand at 3690.860 kb on - strand, within PGA1_c35190 at 3690.860 kb on - strand, within PGA1_c35190 at 3690.880 kb on + strand, within PGA1_c35190 at 3690.923 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.995 kb on - strand, within PGA1_c35190 at 3691.029 kb on - strand, within PGA1_c35190 at 3691.036 kb on - strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.198 kb on - strand, within PGA1_c35190 at 3691.209 kb on + strand, within PGA1_c35190 at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.300 kb on + strand at 3691.311 kb on - strand at 3691.316 kb on - strand at 3691.320 kb on + strand at 3691.330 kb on + strand at 3691.395 kb on - strand at 3691.416 kb on + strand at 3691.435 kb on - strand at 3691.460 kb on - strand, within PGA1_c35200 at 3691.468 kb on - strand, within PGA1_c35200 at 3691.472 kb on + strand, within PGA1_c35200 at 3691.480 kb on - strand, within PGA1_c35200 at 3691.488 kb on - strand, within PGA1_c35200 at 3691.600 kb on - strand, within PGA1_c35200 at 3691.645 kb on - strand, within PGA1_c35200 at 3691.689 kb on + strand, within PGA1_c35200 at 3691.749 kb on - strand, within PGA1_c35200 at 3691.794 kb on - strand, within PGA1_c35200 at 3691.818 kb on - strand, within PGA1_c35200 at 3691.858 kb on - strand, within PGA1_c35200 at 3691.878 kb on + strand, within PGA1_c35200 at 3691.910 kb on - strand, within PGA1_c35200 at 3692.129 kb on + strand at 3692.139 kb on + strand at 3692.139 kb on + strand at 3692.154 kb on + strand, within PGA1_c35210 at 3692.175 kb on - strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 3,689,989 + PGA1_c35170 0.82 +0.6 3,690,007 + PGA1_c35170 0.85 -0.9 3,690,093 + -0.3 3,690,096 + -2.0 3,690,096 + -0.8 3,690,096 + +0.9 3,690,098 + +0.7 3,690,104 - -0.7 3,690,161 - -0.6 3,690,186 + +0.3 3,690,186 + +1.7 3,690,186 + -0.3 3,690,194 - +2.0 3,690,194 - +1.2 3,690,194 - +2.0 3,690,204 + +1.4 3,690,225 - +0.9 3,690,225 - +1.4 3,690,225 - +2.8 3,690,225 - +0.1 3,690,766 + -1.4 3,690,771 + -0.3 3,690,779 + -1.7 3,690,860 - PGA1_c35190 0.18 -1.9 3,690,860 - PGA1_c35190 0.18 -3.3 3,690,880 + PGA1_c35190 0.22 -2.1 3,690,923 + PGA1_c35190 0.31 -1.9 3,690,987 + PGA1_c35190 0.43 -1.8 3,690,987 + PGA1_c35190 0.43 -1.2 3,690,995 - PGA1_c35190 0.45 -2.8 3,691,029 - PGA1_c35190 0.51 -1.2 3,691,036 - PGA1_c35190 0.53 -1.1 3,691,184 + PGA1_c35190 0.82 +1.3 3,691,184 + PGA1_c35190 0.82 -0.5 3,691,184 + PGA1_c35190 0.82 -3.6 3,691,198 - PGA1_c35190 0.85 -0.6 3,691,209 + PGA1_c35190 0.87 -3.2 3,691,292 - -2.0 3,691,292 - -3.4 3,691,292 - -0.5 3,691,300 + -0.4 3,691,311 - -0.6 3,691,316 - -3.1 3,691,320 + -1.5 3,691,330 + -3.8 3,691,395 - -2.2 3,691,416 + -1.8 3,691,435 - -3.6 3,691,460 - PGA1_c35200 0.11 -1.5 3,691,468 - PGA1_c35200 0.12 -2.8 3,691,472 + PGA1_c35200 0.12 -3.5 3,691,480 - PGA1_c35200 0.14 -2.9 3,691,488 - PGA1_c35200 0.15 -2.1 3,691,600 - PGA1_c35200 0.32 -2.5 3,691,645 - PGA1_c35200 0.39 -3.9 3,691,689 + PGA1_c35200 0.46 -2.8 3,691,749 - PGA1_c35200 0.55 -2.6 3,691,794 - PGA1_c35200 0.61 -1.7 3,691,818 - PGA1_c35200 0.65 -1.5 3,691,858 - PGA1_c35200 0.71 -3.8 3,691,878 + PGA1_c35200 0.74 -3.0 3,691,910 - PGA1_c35200 0.79 -3.2 3,692,129 + -1.0 3,692,139 + -1.5 3,692,139 + +0.1 3,692,154 + PGA1_c35210 0.11 -2.4 3,692,175 - PGA1_c35210 0.12 -0.7 3,692,273 + PGA1_c35210 0.22 +1.3 3,692,273 + PGA1_c35210 0.22 -0.9 3,692,273 + PGA1_c35210 0.22 +1.0 3,692,273 + PGA1_c35210 0.22 +1.1 3,692,273 + PGA1_c35210 0.22 -0.8 3,692,273 + PGA1_c35210 0.22 +0.2 3,692,273 + PGA1_c35210 0.22 -0.1 3,692,273 + PGA1_c35210 0.22 -0.2 3,692,273 + PGA1_c35210 0.22 +0.5 3,692,273 + PGA1_c35210 0.22 +1.2 3,692,273 + PGA1_c35210 0.22 -0.7 3,692,273 + PGA1_c35210 0.22 -0.9 3,692,273 + PGA1_c35210 0.22 +0.2 3,692,273 + PGA1_c35210 0.22 +1.7 3,692,273 + PGA1_c35210 0.22 +0.5 3,692,273 + PGA1_c35210 0.22 -0.3 3,692,273 + PGA1_c35210 0.22 +1.1
Or see this region's nucleotide sequence