Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32880 and PGA1_c32890 are separated by 90 nucleotides PGA1_c32890 and PGA1_c32900 are separated by 22 nucleotides PGA1_c32900 and PGA1_c32910 overlap by 1 nucleotides PGA1_c32910 and PGA1_c32920 are separated by 3 nucleotides PGA1_c32920 and PGA1_c32930 are separated by 114 nucleotides
PGA1_c32880: PGA1_c32880 - putative ribosomal RNA small subunit methyltransferase B, at 3,449,245 to 3,450,510
_c32880
PGA1_c32890: PGA1_c32890 - Protein of unknown function (DUF1674)., at 3,450,601 to 3,450,795
_c32890
PGA1_c32900: PGA1_c32900 - Antibiotic biosynthesis monooxygenase., at 3,450,818 to 3,450,973
_c32900
PGA1_c32910: PGA1_c32910 - hypothetical protein, at 3,450,973 to 3,451,152
_c32910
PGA1_c32920: PGA1_c32920 - dihydrodipicolinate reductase DapB, at 3,451,156 to 3,451,977
_c32920
PGA1_c32930: PGA1_c32930 - ribosome-binding factor A, at 3,452,092 to 3,452,487
_c32930
Position (kb)
3450
3451
3452 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3450.029 kb on + strand, within PGA1_c32880 at 3450.029 kb on + strand, within PGA1_c32880 at 3450.239 kb on + strand, within PGA1_c32880 at 3450.239 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.245 kb on + strand, within PGA1_c32880 at 3450.247 kb on - strand, within PGA1_c32880 at 3450.253 kb on - strand, within PGA1_c32880 at 3450.253 kb on - strand, within PGA1_c32880 at 3450.263 kb on - strand, within PGA1_c32880 at 3450.263 kb on - strand, within PGA1_c32880 at 3450.286 kb on - strand, within PGA1_c32880 at 3450.286 kb on - strand, within PGA1_c32880 at 3450.305 kb on + strand, within PGA1_c32880 at 3450.333 kb on - strand, within PGA1_c32880 at 3450.369 kb on + strand, within PGA1_c32880 at 3450.390 kb on + strand at 3450.390 kb on + strand at 3450.390 kb on + strand at 3450.457 kb on - strand at 3450.471 kb on + strand at 3450.471 kb on + strand at 3450.490 kb on + strand at 3450.508 kb on - strand at 3450.611 kb on - strand at 3450.801 kb on + strand at 3450.806 kb on + strand at 3450.806 kb on + strand at 3450.814 kb on - strand at 3450.814 kb on - strand at 3450.851 kb on - strand, within PGA1_c32900 at 3450.874 kb on - strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on + strand, within PGA1_c32900 at 3450.897 kb on - strand, within PGA1_c32900 at 3450.905 kb on - strand, within PGA1_c32900 at 3450.905 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3450.910 kb on - strand, within PGA1_c32900 at 3451.057 kb on - strand, within PGA1_c32910 at 3451.106 kb on + strand, within PGA1_c32910 at 3451.108 kb on + strand, within PGA1_c32910 at 3451.116 kb on - strand, within PGA1_c32910 at 3451.136 kb on - strand at 3451.159 kb on - strand at 3452.030 kb on + strand at 3452.043 kb on + strand at 3452.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 3,450,029 + PGA1_c32880 0.62 -0.4 3,450,029 + PGA1_c32880 0.62 -0.9 3,450,239 + PGA1_c32880 0.79 -1.1 3,450,239 + PGA1_c32880 0.79 -1.1 3,450,245 + PGA1_c32880 0.79 -1.1 3,450,245 + PGA1_c32880 0.79 +0.3 3,450,245 + PGA1_c32880 0.79 -3.3 3,450,245 + PGA1_c32880 0.79 -2.3 3,450,247 - PGA1_c32880 0.79 -2.0 3,450,253 - PGA1_c32880 0.80 -0.9 3,450,253 - PGA1_c32880 0.80 -2.6 3,450,263 - PGA1_c32880 0.80 -1.8 3,450,263 - PGA1_c32880 0.80 -1.8 3,450,286 - PGA1_c32880 0.82 -0.8 3,450,286 - PGA1_c32880 0.82 -0.6 3,450,305 + PGA1_c32880 0.84 -1.0 3,450,333 - PGA1_c32880 0.86 -0.6 3,450,369 + PGA1_c32880 0.89 -4.0 3,450,390 + -1.0 3,450,390 + -4.3 3,450,390 + -0.2 3,450,457 - -0.4 3,450,471 + -0.5 3,450,471 + -0.8 3,450,490 + -0.7 3,450,508 - -1.0 3,450,611 - -0.8 3,450,801 + -1.6 3,450,806 + -0.5 3,450,806 + -1.4 3,450,814 - +0.4 3,450,814 - +0.5 3,450,851 - PGA1_c32900 0.21 -0.3 3,450,874 - PGA1_c32900 0.36 +0.8 3,450,897 + PGA1_c32900 0.51 +0.3 3,450,897 + PGA1_c32900 0.51 -0.6 3,450,897 + PGA1_c32900 0.51 +0.1 3,450,897 - PGA1_c32900 0.51 +1.6 3,450,905 - PGA1_c32900 0.56 -2.3 3,450,905 - PGA1_c32900 0.56 +0.4 3,450,910 - PGA1_c32900 0.59 -0.7 3,450,910 - PGA1_c32900 0.59 -1.1 3,450,910 - PGA1_c32900 0.59 -1.5 3,451,057 - PGA1_c32910 0.47 -3.2 3,451,106 + PGA1_c32910 0.74 +0.9 3,451,108 + PGA1_c32910 0.75 +0.1 3,451,116 - PGA1_c32910 0.79 +0.5 3,451,136 - +1.2 3,451,159 - -0.6 3,452,030 + -1.7 3,452,043 + +0.5 3,452,123 - -1.3
Or see this region's nucleotide sequence