Experiment: Cephalothin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2304 and Psest_2305 are separated by 6 nucleotides Psest_2305 and Psest_2306 overlap by 4 nucleotides Psest_2306 and Psest_2307 are separated by 132 nucleotides
Psest_2304: Psest_2304 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 2,455,315 to 2,455,809
_2304
Psest_2305: Psest_2305 - UDP-2,3-diacylglucosamine hydrolase, at 2,455,816 to 2,456,538
_2305
Psest_2306: Psest_2306 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 2,456,535 to 2,457,185
_2306
Psest_2307: Psest_2307 - Universal stress protein UspA and related nucleotide-binding proteins, at 2,457,318 to 2,458,178
_2307
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2456.536 kb on + strand at 2456.537 kb on - strand at 2456.659 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.675 kb on - strand, within Psest_2306 at 2456.717 kb on - strand, within Psest_2306 at 2456.727 kb on + strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.988 kb on + strand, within Psest_2306 at 2456.988 kb on + strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.170 kb on - strand at 2457.170 kb on - strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.251 kb on + strand at 2457.251 kb on + strand at 2457.251 kb on + strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.281 kb on - strand at 2457.281 kb on - strand at 2457.282 kb on + strand at 2457.282 kb on + strand at 2457.283 kb on - strand at 2457.283 kb on - strand at 2457.283 kb on - strand at 2457.686 kb on + strand, within Psest_2307 at 2457.686 kb on + strand, within Psest_2307 at 2457.687 kb on - strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.884 kb on - strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.965 kb on + strand, within Psest_2307 at 2457.966 kb on - strand, within Psest_2307 at 2458.016 kb on + strand, within Psest_2307 at 2458.017 kb on - strand, within Psest_2307 at 2458.036 kb on + strand, within Psest_2307 at 2458.037 kb on - strand, within Psest_2307 at 2458.082 kb on + strand, within Psest_2307
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.04 mg/ml remove 2,456,536 + +1.9 2,456,537 - +0.3 2,456,659 + Psest_2306 0.19 -0.2 2,456,674 + Psest_2306 0.21 +0.2 2,456,674 + Psest_2306 0.21 -0.1 2,456,674 + Psest_2306 0.21 -1.2 2,456,675 - Psest_2306 0.22 +0.5 2,456,717 - Psest_2306 0.28 -0.7 2,456,727 + Psest_2306 0.29 -0.2 2,456,728 - Psest_2306 0.30 +1.5 2,456,728 - Psest_2306 0.30 -0.2 2,456,988 + Psest_2306 0.70 -0.2 2,456,988 + Psest_2306 0.70 -0.9 2,456,989 - Psest_2306 0.70 +0.0 2,456,989 - Psest_2306 0.70 +1.3 2,456,989 - Psest_2306 0.70 -0.4 2,456,989 - Psest_2306 0.70 -1.1 2,457,060 + Psest_2306 0.81 -0.8 2,457,060 + Psest_2306 0.81 -1.6 2,457,060 + Psest_2306 0.81 +0.8 2,457,060 + Psest_2306 0.81 -2.1 2,457,060 + Psest_2306 0.81 -0.6 2,457,061 - Psest_2306 0.81 -1.8 2,457,061 - Psest_2306 0.81 -0.3 2,457,061 - Psest_2306 0.81 -2.0 2,457,061 - Psest_2306 0.81 -0.8 2,457,061 - Psest_2306 0.81 -0.9 2,457,061 - Psest_2306 0.81 +0.0 2,457,061 - Psest_2306 0.81 -0.5 2,457,061 - Psest_2306 0.81 -1.4 2,457,061 - Psest_2306 0.81 +1.0 2,457,061 - Psest_2306 0.81 -0.6 2,457,061 - Psest_2306 0.81 -0.3 2,457,170 - -1.5 2,457,170 - -1.0 2,457,214 + -0.9 2,457,214 + -1.1 2,457,214 + +0.3 2,457,214 + +0.2 2,457,215 - -2.4 2,457,215 - +0.1 2,457,215 - -0.6 2,457,215 - +0.7 2,457,215 - -0.9 2,457,215 - -0.2 2,457,215 - +1.0 2,457,215 - +2.0 2,457,251 + -1.1 2,457,251 + -1.6 2,457,251 + -1.0 2,457,252 - +0.8 2,457,252 - +0.6 2,457,252 - +0.6 2,457,252 - -0.5 2,457,252 - -0.7 2,457,252 - -1.7 2,457,281 - -3.0 2,457,281 - +0.0 2,457,282 + -0.6 2,457,282 + -1.7 2,457,283 - -0.3 2,457,283 - +0.9 2,457,283 - -2.2 2,457,686 + Psest_2307 0.43 -0.9 2,457,686 + Psest_2307 0.43 -0.8 2,457,687 - Psest_2307 0.43 -1.7 2,457,829 + Psest_2307 0.59 +0.1 2,457,829 + Psest_2307 0.59 +0.2 2,457,829 + Psest_2307 0.59 +0.6 2,457,884 - Psest_2307 0.66 +0.6 2,457,961 + Psest_2307 0.75 +0.1 2,457,961 + Psest_2307 0.75 +2.2 2,457,961 + Psest_2307 0.75 +0.7 2,457,961 + Psest_2307 0.75 +0.6 2,457,961 + Psest_2307 0.75 -0.4 2,457,961 + Psest_2307 0.75 -1.0 2,457,961 + Psest_2307 0.75 +0.5 2,457,962 - Psest_2307 0.75 -0.7 2,457,962 - Psest_2307 0.75 -1.7 2,457,962 - Psest_2307 0.75 -0.0 2,457,962 - Psest_2307 0.75 -0.3 2,457,962 - Psest_2307 0.75 -2.5 2,457,962 - Psest_2307 0.75 +1.1 2,457,962 - Psest_2307 0.75 +0.9 2,457,965 + Psest_2307 0.75 +0.5 2,457,966 - Psest_2307 0.75 -0.4 2,458,016 + Psest_2307 0.81 -0.2 2,458,017 - Psest_2307 0.81 -0.0 2,458,036 + Psest_2307 0.83 +0.6 2,458,037 - Psest_2307 0.84 -0.8 2,458,082 + Psest_2307 0.89 +0.0
Or see this region's nucleotide sequence