Experiment: m.b. Sisomicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20870 and PGA1_c20880 are separated by 189 nucleotides PGA1_c20880 and PGA1_c20890 are separated by 118 nucleotides
PGA1_c20870: PGA1_c20870 - molybdenum hydroxylase family protein, large subunit, at 2,163,018 to 2,165,381
_c20870
PGA1_c20880: PGA1_c20880 - molybdenum hydroxylase family protein, small subunit, at 2,165,571 to 2,166,056
_c20880
PGA1_c20890: PGA1_c20890 - Molybdopterin biosynthesis enzyme, at 2,166,175 to 2,167,179
_c20890
Position (kb)
2165
2166
2167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2164.646 kb on + strand, within PGA1_c20870 at 2164.654 kb on - strand, within PGA1_c20870 at 2164.657 kb on + strand, within PGA1_c20870 at 2164.667 kb on + strand, within PGA1_c20870 at 2164.667 kb on + strand, within PGA1_c20870 at 2164.667 kb on + strand, within PGA1_c20870 at 2164.808 kb on + strand, within PGA1_c20870 at 2164.816 kb on - strand, within PGA1_c20870 at 2165.013 kb on - strand, within PGA1_c20870 at 2165.015 kb on + strand, within PGA1_c20870 at 2165.065 kb on + strand, within PGA1_c20870 at 2165.065 kb on + strand, within PGA1_c20870 at 2165.089 kb on - strand, within PGA1_c20870 at 2165.129 kb on + strand, within PGA1_c20870 at 2165.137 kb on + strand, within PGA1_c20870 at 2165.145 kb on - strand at 2165.145 kb on - strand at 2165.192 kb on + strand at 2165.210 kb on + strand at 2165.300 kb on - strand at 2165.328 kb on + strand at 2165.336 kb on - strand at 2165.353 kb on - strand at 2165.373 kb on - strand at 2165.391 kb on - strand at 2165.414 kb on + strand at 2165.414 kb on + strand at 2165.414 kb on + strand at 2165.472 kb on - strand at 2165.472 kb on - strand at 2165.594 kb on - strand at 2165.598 kb on - strand at 2165.598 kb on - strand at 2165.602 kb on - strand at 2165.603 kb on - strand at 2165.639 kb on + strand, within PGA1_c20880 at 2165.647 kb on + strand, within PGA1_c20880 at 2165.651 kb on + strand, within PGA1_c20880 at 2165.661 kb on + strand, within PGA1_c20880 at 2165.662 kb on + strand, within PGA1_c20880 at 2165.670 kb on - strand, within PGA1_c20880 at 2165.704 kb on - strand, within PGA1_c20880 at 2165.715 kb on - strand, within PGA1_c20880 at 2165.736 kb on - strand, within PGA1_c20880 at 2165.969 kb on - strand, within PGA1_c20880 at 2166.065 kb on + strand at 2166.065 kb on + strand at 2166.093 kb on + strand at 2166.110 kb on - strand at 2166.111 kb on - strand at 2166.124 kb on + strand at 2166.171 kb on - strand at 2166.171 kb on - strand at 2166.198 kb on - strand at 2166.202 kb on - strand at 2166.340 kb on + strand, within PGA1_c20890 at 2166.340 kb on + strand, within PGA1_c20890 at 2166.472 kb on + strand, within PGA1_c20890 at 2166.739 kb on + strand, within PGA1_c20890 at 2166.747 kb on + strand, within PGA1_c20890 at 2166.747 kb on - strand, within PGA1_c20890 at 2166.778 kb on + strand, within PGA1_c20890 at 2166.809 kb on + strand, within PGA1_c20890 at 2166.834 kb on + strand, within PGA1_c20890 at 2166.866 kb on + strand, within PGA1_c20890 at 2166.869 kb on - strand, within PGA1_c20890 at 2166.877 kb on + strand, within PGA1_c20890 at 2166.885 kb on - strand, within PGA1_c20890 at 2166.885 kb on - strand, within PGA1_c20890 at 2166.939 kb on + strand, within PGA1_c20890 at 2167.007 kb on - strand, within PGA1_c20890 at 2167.031 kb on - strand, within PGA1_c20890
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.005 mg/ml remove 2,164,646 + PGA1_c20870 0.69 +0.1 2,164,654 - PGA1_c20870 0.69 -1.6 2,164,657 + PGA1_c20870 0.69 -1.5 2,164,667 + PGA1_c20870 0.70 -2.5 2,164,667 + PGA1_c20870 0.70 +0.2 2,164,667 + PGA1_c20870 0.70 +0.0 2,164,808 + PGA1_c20870 0.76 -2.1 2,164,816 - PGA1_c20870 0.76 -0.4 2,165,013 - PGA1_c20870 0.84 +1.6 2,165,015 + PGA1_c20870 0.84 +0.0 2,165,065 + PGA1_c20870 0.87 +0.9 2,165,065 + PGA1_c20870 0.87 -0.7 2,165,089 - PGA1_c20870 0.88 +1.4 2,165,129 + PGA1_c20870 0.89 -1.2 2,165,137 + PGA1_c20870 0.90 +1.5 2,165,145 - -0.4 2,165,145 - -0.5 2,165,192 + -0.4 2,165,210 + -0.5 2,165,300 - +0.6 2,165,328 + -1.3 2,165,336 - -0.1 2,165,353 - -2.2 2,165,373 - -1.7 2,165,391 - -0.3 2,165,414 + -0.1 2,165,414 + -1.3 2,165,414 + -1.3 2,165,472 - -0.0 2,165,472 - -0.0 2,165,594 - -1.9 2,165,598 - +0.1 2,165,598 - -1.4 2,165,602 - -2.3 2,165,603 - -0.7 2,165,639 + PGA1_c20880 0.14 -1.8 2,165,647 + PGA1_c20880 0.16 +1.2 2,165,651 + PGA1_c20880 0.16 +0.3 2,165,661 + PGA1_c20880 0.19 -1.3 2,165,662 + PGA1_c20880 0.19 +0.2 2,165,670 - PGA1_c20880 0.20 -1.8 2,165,704 - PGA1_c20880 0.27 -2.6 2,165,715 - PGA1_c20880 0.30 -0.3 2,165,736 - PGA1_c20880 0.34 -2.4 2,165,969 - PGA1_c20880 0.82 -0.8 2,166,065 + -1.4 2,166,065 + -2.0 2,166,093 + -1.3 2,166,110 - -3.0 2,166,111 - +1.7 2,166,124 + +0.8 2,166,171 - -1.8 2,166,171 - +0.3 2,166,198 - +0.0 2,166,202 - +0.4 2,166,340 + PGA1_c20890 0.16 +0.4 2,166,340 + PGA1_c20890 0.16 +0.8 2,166,472 + PGA1_c20890 0.30 -1.8 2,166,739 + PGA1_c20890 0.56 -0.5 2,166,747 + PGA1_c20890 0.57 -0.3 2,166,747 - PGA1_c20890 0.57 +0.2 2,166,778 + PGA1_c20890 0.60 +1.9 2,166,809 + PGA1_c20890 0.63 -0.4 2,166,834 + PGA1_c20890 0.66 +0.7 2,166,866 + PGA1_c20890 0.69 -2.3 2,166,869 - PGA1_c20890 0.69 +0.8 2,166,877 + PGA1_c20890 0.70 +1.4 2,166,885 - PGA1_c20890 0.71 -1.5 2,166,885 - PGA1_c20890 0.71 -0.0 2,166,939 + PGA1_c20890 0.76 +0.2 2,167,007 - PGA1_c20890 0.83 -1.5 2,167,031 - PGA1_c20890 0.85 +1.6
Or see this region's nucleotide sequence