Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0102

Experiment: Cephalothin 0.04 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0101 and Psest_0102 are separated by 173 nucleotidesPsest_0102 and Psest_0103 overlap by 1 nucleotidesPsest_0103 and Psest_0104 are separated by 2 nucleotides Psest_0101: Psest_0101 - Short-chain alcohol dehydrogenase of unknown specificity, at 100,412 to 101,176 _0101 Psest_0102: Psest_0102 - malonate decarboxylase, alpha subunit, at 101,350 to 103,017 _0102 Psest_0103: Psest_0103 - triphosphoribosyl-dephospho-CoA synthase MdcB, at 103,017 to 103,895 _0103 Psest_0104: Psest_0104 - malonate decarboxylase acyl carrier protein, at 103,898 to 104,197 _0104 Position (kb) 101 102 103 104Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 100.428 kb on + strandat 100.428 kb on + strandat 100.428 kb on + strandat 100.429 kb on - strandat 100.429 kb on - strandat 100.429 kb on - strandat 100.868 kb on + strand, within Psest_0101at 100.907 kb on - strand, within Psest_0101at 101.078 kb on + strand, within Psest_0101at 101.078 kb on + strand, within Psest_0101at 101.078 kb on + strand, within Psest_0101at 101.078 kb on + strand, within Psest_0101at 101.078 kb on + strand, within Psest_0101at 101.079 kb on - strand, within Psest_0101at 101.079 kb on - strand, within Psest_0101at 101.079 kb on - strand, within Psest_0101at 101.174 kb on + strandat 101.174 kb on + strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.175 kb on - strandat 101.187 kb on + strandat 101.248 kb on - strandat 101.311 kb on + strandat 101.311 kb on + strandat 101.312 kb on - strandat 101.312 kb on - strandat 101.312 kb on - strandat 101.312 kb on - strandat 101.312 kb on - strandat 101.312 kb on - strandat 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.749 kb on + strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.750 kb on - strand, within Psest_0102at 101.761 kb on + strand, within Psest_0102at 101.761 kb on + strand, within Psest_0102at 101.761 kb on + strand, within Psest_0102at 101.762 kb on - strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.839 kb on + strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 101.840 kb on - strand, within Psest_0102at 102.001 kb on + strand, within Psest_0102at 102.001 kb on + strand, within Psest_0102at 102.085 kb on + strand, within Psest_0102at 102.085 kb on + strand, within Psest_0102at 102.085 kb on + strand, within Psest_0102at 102.085 kb on + strand, within Psest_0102at 102.085 kb on + strand, within Psest_0102at 102.086 kb on - strand, within Psest_0102at 102.086 kb on - strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.301 kb on + strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.302 kb on - strand, within Psest_0102at 102.388 kb on + strand, within Psest_0102at 102.388 kb on + strand, within Psest_0102at 102.389 kb on - strand, within Psest_0102at 102.715 kb on + strand, within Psest_0102at 102.715 kb on + strand, within Psest_0102at 102.715 kb on + strand, within Psest_0102at 102.716 kb on - strand, within Psest_0102at 102.716 kb on - strand, within Psest_0102at 102.760 kb on + strand, within Psest_0102at 102.761 kb on - strand, within Psest_0102at 102.766 kb on + strand, within Psest_0102at 102.766 kb on + strand, within Psest_0102at 102.766 kb on + strand, within Psest_0102at 102.766 kb on + strand, within Psest_0102at 102.766 kb on + strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.767 kb on - strand, within Psest_0102at 102.985 kb on + strandat 102.985 kb on + strandat 102.985 kb on + strandat 102.985 kb on + strandat 102.985 kb on + strandat 102.985 kb on + strandat 102.986 kb on - strandat 102.986 kb on - strandat 102.986 kb on - strandat 103.327 kb on + strand, within Psest_0103at 103.327 kb on + strand, within Psest_0103at 103.328 kb on - strand, within Psest_0103at 103.328 kb on - strand, within Psest_0103at 103.466 kb on + strand, within Psest_0103at 103.466 kb on + strand, within Psest_0103

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.04 mg/ml
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100,428 + +1.4
100,428 + +1.0
100,428 + +1.7
100,429 - -1.4
100,429 - +0.4
100,429 - +0.4
100,868 + Psest_0101 0.60 +1.5
100,907 - Psest_0101 0.65 +0.8
101,078 + Psest_0101 0.87 -2.1
101,078 + Psest_0101 0.87 +2.4
101,078 + Psest_0101 0.87 +1.0
101,078 + Psest_0101 0.87 -1.2
101,078 + Psest_0101 0.87 -1.0
101,079 - Psest_0101 0.87 +1.7
101,079 - Psest_0101 0.87 +2.4
101,079 - Psest_0101 0.87 +1.4
101,174 + -0.5
101,174 + +0.1
101,175 - +0.6
101,175 - +3.0
101,175 - +1.3
101,175 - +2.0
101,175 - +0.4
101,175 - +0.8
101,175 - -0.4
101,175 - +1.4
101,175 - +0.4
101,175 - +0.4
101,187 + +0.2
101,248 - +1.0
101,311 + -1.4
101,311 + -2.8
101,312 - +0.5
101,312 - -0.3
101,312 - -1.0
101,312 - +2.0
101,312 - +1.4
101,312 - +0.1
101,749 + Psest_0102 0.24 +1.6
101,749 + Psest_0102 0.24 -2.2
101,749 + Psest_0102 0.24 -1.4
101,749 + Psest_0102 0.24 +0.1
101,749 + Psest_0102 0.24 -0.2
101,749 + Psest_0102 0.24 -0.6
101,749 + Psest_0102 0.24 -0.6
101,750 - Psest_0102 0.24 +0.1
101,750 - Psest_0102 0.24 +1.0
101,750 - Psest_0102 0.24 +0.4
101,750 - Psest_0102 0.24 +0.4
101,750 - Psest_0102 0.24 +0.1
101,750 - Psest_0102 0.24 +0.0
101,750 - Psest_0102 0.24 +0.0
101,750 - Psest_0102 0.24 +0.1
101,750 - Psest_0102 0.24 +0.4
101,750 - Psest_0102 0.24 +1.0
101,750 - Psest_0102 0.24 +1.0
101,750 - Psest_0102 0.24 +0.1
101,761 + Psest_0102 0.25 -2.5
101,761 + Psest_0102 0.25 +0.8
101,761 + Psest_0102 0.25 -1.8
101,762 - Psest_0102 0.25 +2.7
101,839 + Psest_0102 0.29 -0.2
101,839 + Psest_0102 0.29 -1.0
101,839 + Psest_0102 0.29 -0.1
101,839 + Psest_0102 0.29 +0.6
101,839 + Psest_0102 0.29 -1.5
101,839 + Psest_0102 0.29 -0.2
101,839 + Psest_0102 0.29 -2.3
101,839 + Psest_0102 0.29 -0.2
101,839 + Psest_0102 0.29 -0.3
101,840 - Psest_0102 0.29 +1.2
101,840 - Psest_0102 0.29 +0.5
101,840 - Psest_0102 0.29 -0.6
101,840 - Psest_0102 0.29 +1.4
101,840 - Psest_0102 0.29 +1.1
101,840 - Psest_0102 0.29 -0.7
102,001 + Psest_0102 0.39 +0.6
102,001 + Psest_0102 0.39 -1.2
102,085 + Psest_0102 0.44 -0.2
102,085 + Psest_0102 0.44 -1.8
102,085 + Psest_0102 0.44 +0.2
102,085 + Psest_0102 0.44 -1.8
102,085 + Psest_0102 0.44 +0.8
102,086 - Psest_0102 0.44 +0.6
102,086 - Psest_0102 0.44 +0.8
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +0.8
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +1.0
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +2.5
102,301 + Psest_0102 0.57 +2.7
102,301 + Psest_0102 0.57 -0.2
102,301 + Psest_0102 0.57 +0.7
102,301 + Psest_0102 0.57 +0.4
102,301 + Psest_0102 0.57 -0.8
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +1.4
102,301 + Psest_0102 0.57 +0.4
102,301 + Psest_0102 0.57 +1.4
102,302 - Psest_0102 0.57 -0.4
102,302 - Psest_0102 0.57 +0.6
102,302 - Psest_0102 0.57 +0.6
102,302 - Psest_0102 0.57 -1.0
102,302 - Psest_0102 0.57 +3.2
102,302 - Psest_0102 0.57 -0.1
102,302 - Psest_0102 0.57 +0.2
102,302 - Psest_0102 0.57 +0.9
102,302 - Psest_0102 0.57 +2.1
102,302 - Psest_0102 0.57 -2.2
102,388 + Psest_0102 0.62 +0.1
102,388 + Psest_0102 0.62 +1.0
102,389 - Psest_0102 0.62 +0.2
102,715 + Psest_0102 0.82 +0.4
102,715 + Psest_0102 0.82 +1.4
102,715 + Psest_0102 0.82 +1.4
102,716 - Psest_0102 0.82 +2.0
102,716 - Psest_0102 0.82 +1.0
102,760 + Psest_0102 0.85 +0.9
102,761 - Psest_0102 0.85 +0.2
102,766 + Psest_0102 0.85 -0.2
102,766 + Psest_0102 0.85 +0.4
102,766 + Psest_0102 0.85 -1.0
102,766 + Psest_0102 0.85 +0.6
102,766 + Psest_0102 0.85 +4.0
102,767 - Psest_0102 0.85 +0.7
102,767 - Psest_0102 0.85 +1.7
102,767 - Psest_0102 0.85 +1.0
102,767 - Psest_0102 0.85 +0.4
102,767 - Psest_0102 0.85 +0.7
102,767 - Psest_0102 0.85 +1.4
102,767 - Psest_0102 0.85 +1.0
102,767 - Psest_0102 0.85 -0.6
102,985 + +0.8
102,985 + +1.0
102,985 + +1.2
102,985 + +3.0
102,985 + +1.4
102,985 + +3.4
102,986 - -0.2
102,986 - -1.2
102,986 - +1.4
103,327 + Psest_0103 0.35 +2.4
103,327 + Psest_0103 0.35 +2.8
103,328 - Psest_0103 0.35 +1.0
103,328 - Psest_0103 0.35 -0.0
103,466 + Psest_0103 0.51 +1.4
103,466 + Psest_0103 0.51 +1.4

Or see this region's nucleotide sequence