Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_3201 and Sama_3202 are separated by 230 nucleotides Sama_3202 and Sama_3203 overlap by 1 nucleotides Sama_3203 and Sama_3204 overlap by 4 nucleotides
Sama_3201: Sama_3201 - Formiminoglutamase (EC 3.5.3.8) (from data) , at 3,791,925 to 3,792,962
_3201
Sama_3202: Sama_3202 - ubiquinone/menaquinone biosynthesis methlytransferase UbiE (RefSeq), at 3,793,193 to 3,793,948
_3202
Sama_3203: Sama_3203 - hypothetical protein (RefSeq), at 3,793,948 to 3,794,565
_3203
Sama_3204: Sama_3204 - ubiquinone biosynthesis protein AarF (RefSeq), at 3,794,562 to 3,796,211
_3204
Position (kb)
3793
3794
3795 Strain fitness (log2 ratio)
-2
-1
0
1 at 3792.994 kb on - strand at 3793.000 kb on + strand at 3793.010 kb on + strand at 3793.010 kb on + strand at 3793.010 kb on + strand at 3793.034 kb on + strand at 3793.040 kb on + strand at 3793.040 kb on + strand at 3793.040 kb on + strand at 3793.132 kb on + strand at 3793.162 kb on + strand at 3793.180 kb on + strand at 3793.180 kb on + strand at 3793.180 kb on + strand at 3793.971 kb on + strand at 3793.971 kb on + strand at 3794.041 kb on + strand, within Sama_3203 at 3794.041 kb on + strand, within Sama_3203 at 3794.185 kb on + strand, within Sama_3203 at 3794.220 kb on + strand, within Sama_3203 at 3794.255 kb on + strand, within Sama_3203 at 3794.255 kb on + strand, within Sama_3203 at 3794.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,792,994 - +0.0 3,793,000 + -1.3 3,793,010 + +1.0 3,793,010 + +0.7 3,793,010 + -0.9 3,793,034 + +1.0 3,793,040 + +0.6 3,793,040 + +0.0 3,793,040 + -0.4 3,793,132 + -0.9 3,793,162 + +0.8 3,793,180 + -0.6 3,793,180 + -1.0 3,793,180 + -0.5 3,793,971 + -0.7 3,793,971 + -2.1 3,794,041 + Sama_3203 0.15 -1.1 3,794,041 + Sama_3203 0.15 -0.8 3,794,185 + Sama_3203 0.38 +0.0 3,794,220 + Sama_3203 0.44 -0.8 3,794,255 + Sama_3203 0.50 -1.4 3,794,255 + Sama_3203 0.50 +0.8 3,794,543 + +0.9
Or see this region's nucleotide sequence