Experiment: Roth35v2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotides BWI76_RS22240 and BWI76_RS22245 overlap by 8 nucleotides BWI76_RS22245 and BWI76_RS22250 overlap by 38 nucleotides
BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327
_RS22235
BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169
_RS22240
BWI76_RS22245: BWI76_RS22245 - arabinose 5-phosphate isomerase GutQ, at 4,636,162 to 4,637,127
_RS22245
BWI76_RS22250: BWI76_RS22250 - nitric oxide reductase transcription regulator, at 4,637,090 to 4,638,634
_RS22250
Position (kb)
4636
4637
4638 Strain fitness (log2 ratio)
-2
-1
0
1 at 4635.182 kb on - strand, within BWI76_RS22235 at 4635.467 kb on - strand at 4635.495 kb on - strand, within BWI76_RS22240 at 4635.583 kb on - strand, within BWI76_RS22240 at 4635.705 kb on + strand, within BWI76_RS22240 at 4635.964 kb on - strand, within BWI76_RS22240 at 4636.176 kb on + strand at 4636.196 kb on + strand at 4636.356 kb on - strand, within BWI76_RS22245 at 4636.472 kb on - strand, within BWI76_RS22245 at 4636.513 kb on - strand, within BWI76_RS22245 at 4636.622 kb on + strand, within BWI76_RS22245 at 4636.623 kb on + strand, within BWI76_RS22245 at 4636.835 kb on - strand, within BWI76_RS22245 at 4636.847 kb on + strand, within BWI76_RS22245 at 4637.022 kb on - strand, within BWI76_RS22245 at 4637.163 kb on - strand at 4637.358 kb on - strand, within BWI76_RS22250 at 4637.886 kb on + strand, within BWI76_RS22250 at 4638.004 kb on + strand, within BWI76_RS22250 at 4638.077 kb on + strand, within BWI76_RS22250 at 4638.102 kb on + strand, within BWI76_RS22250 at 4638.119 kb on - strand, within BWI76_RS22250
Per-strain Table
Position Strand Gene LocusTag Fraction Roth35v2 remove 4,635,182 - BWI76_RS22235 0.59 -0.6 4,635,467 - +0.8 4,635,495 - BWI76_RS22240 0.13 -1.6 4,635,583 - BWI76_RS22240 0.24 +0.4 4,635,705 + BWI76_RS22240 0.40 -0.2 4,635,964 - BWI76_RS22240 0.73 -1.2 4,636,176 + -1.6 4,636,196 + +0.0 4,636,356 - BWI76_RS22245 0.20 -0.8 4,636,472 - BWI76_RS22245 0.32 -1.1 4,636,513 - BWI76_RS22245 0.36 -0.2 4,636,622 + BWI76_RS22245 0.48 -1.9 4,636,623 + BWI76_RS22245 0.48 -1.3 4,636,835 - BWI76_RS22245 0.70 -1.7 4,636,847 + BWI76_RS22245 0.71 -1.3 4,637,022 - BWI76_RS22245 0.89 -0.9 4,637,163 - +0.2 4,637,358 - BWI76_RS22250 0.17 -0.7 4,637,886 + BWI76_RS22250 0.52 +0.5 4,638,004 + BWI76_RS22250 0.59 -0.7 4,638,077 + BWI76_RS22250 0.64 -0.4 4,638,102 + BWI76_RS22250 0.66 -1.0 4,638,119 - BWI76_RS22250 0.67 +0.0
Or see this region's nucleotide sequence