Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00090

Experiment: KP1_E1_R2_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotidesBWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotidesBWI76_RS00090 and BWI76_RS00095 overlap by 32 nucleotides BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581 _RS00080 BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042 _RS00085 BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 BWI76_RS00095: BWI76_RS00095 - DNA-binding transcriptional regulator, at 18,362 to 19,321 _RS00095 Position (kb) 17 18 19Strain fitness (log2 ratio) -2 -1 0 1 2 3at 16.225 kb on + strand, within BWI76_RS00080at 16.451 kb on + strand, within BWI76_RS00080at 16.461 kb on + strand, within BWI76_RS00080at 16.469 kb on - strand, within BWI76_RS00080at 16.514 kb on - strandat 16.529 kb on - strandat 16.601 kb on - strandat 16.609 kb on + strandat 16.616 kb on + strandat 16.631 kb on + strand, within BWI76_RS00085at 16.631 kb on + strand, within BWI76_RS00085at 16.932 kb on - strand, within BWI76_RS00085at 17.020 kb on - strandat 17.088 kb on - strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.184 kb on - strandat 17.184 kb on - strandat 17.214 kb on - strandat 17.244 kb on + strandat 17.252 kb on - strandat 17.252 kb on - strandat 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090at 17.672 kb on + strand, within BWI76_RS00090at 17.683 kb on + strand, within BWI76_RS00090at 17.688 kb on + strand, within BWI76_RS00090at 17.696 kb on - strand, within BWI76_RS00090at 17.881 kb on - strand, within BWI76_RS00090at 17.963 kb on + strand, within BWI76_RS00090at 18.025 kb on - strand, within BWI76_RS00090at 18.069 kb on - strand, within BWI76_RS00090at 18.111 kb on + strand, within BWI76_RS00090at 18.167 kb on + strand, within BWI76_RS00090at 18.217 kb on - strand, within BWI76_RS00090at 18.219 kb on - strand, within BWI76_RS00090at 18.343 kb on - strandat 18.381 kb on + strandat 18.544 kb on - strandat 18.570 kb on + strand, within BWI76_RS00095at 18.904 kb on + strand, within BWI76_RS00095at 19.096 kb on + strand, within BWI76_RS00095at 19.227 kb on + strandat 19.300 kb on + strandat 19.308 kb on - strandat 19.308 kb on - strandat 19.313 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KP1_E1_R2_1
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16,225 + BWI76_RS00080 0.59 +0.9
16,451 + BWI76_RS00080 0.85 -0.2
16,461 + BWI76_RS00080 0.86 -0.2
16,469 - BWI76_RS00080 0.87 -1.9
16,514 - +1.7
16,529 - +1.1
16,601 - -0.6
16,609 + -2.6
16,616 + -2.5
16,631 + BWI76_RS00085 0.11 -0.0
16,631 + BWI76_RS00085 0.11 -0.2
16,932 - BWI76_RS00085 0.76 -0.3
17,020 - -0.3
17,088 - +2.1
17,176 + +0.3
17,176 + -0.7
17,176 + +0.4
17,176 + -1.8
17,184 - -0.2
17,184 - -0.4
17,214 - +1.0
17,244 + -0.4
17,252 - -0.3
17,252 - -0.4
17,498 - BWI76_RS00090 0.24 -0.6
17,539 + BWI76_RS00090 0.28 -0.6
17,546 + BWI76_RS00090 0.28 -1.1
17,672 + BWI76_RS00090 0.39 -1.1
17,683 + BWI76_RS00090 0.40 +3.1
17,688 + BWI76_RS00090 0.40 -0.9
17,696 - BWI76_RS00090 0.41 -0.6
17,881 - BWI76_RS00090 0.57 +0.1
17,963 + BWI76_RS00090 0.64 -1.2
18,025 - BWI76_RS00090 0.69 -0.1
18,069 - BWI76_RS00090 0.73 -0.2
18,111 + BWI76_RS00090 0.76 -0.5
18,167 + BWI76_RS00090 0.81 +0.4
18,217 - BWI76_RS00090 0.85 -1.4
18,219 - BWI76_RS00090 0.85 -0.1
18,343 - +1.4
18,381 + -0.4
18,544 - +2.1
18,570 + BWI76_RS00095 0.22 +0.5
18,904 + BWI76_RS00095 0.56 +0.0
19,096 + BWI76_RS00095 0.76 -0.5
19,227 + +0.0
19,300 + +0.2
19,308 - +1.1
19,308 - -0.1
19,313 - +0.7

Or see this region's nucleotide sequence