Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19385

Experiment: MM10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19380 and BWI76_RS19385 are separated by 363 nucleotides BWI76_RS19380: BWI76_RS19380 - hydroxyethylthiazole kinase, at 4,039,526 to 4,040,299 _RS19380 BWI76_RS19385: BWI76_RS19385 - MBL fold hydrolase, at 4,040,663 to 4,042,639 _RS19385 Position (kb) 4040 4041 4042 4043Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4039.865 kb on + strand, within BWI76_RS19380at 4039.906 kb on - strand, within BWI76_RS19380at 4040.019 kb on + strand, within BWI76_RS19380at 4040.225 kb on + strandat 4040.298 kb on - strandat 4040.406 kb on + strandat 4040.496 kb on + strandat 4040.496 kb on + strandat 4040.504 kb on - strandat 4040.504 kb on - strandat 4040.514 kb on - strandat 4040.867 kb on - strand, within BWI76_RS19385at 4041.393 kb on - strand, within BWI76_RS19385at 4041.588 kb on + strand, within BWI76_RS19385at 4041.588 kb on + strand, within BWI76_RS19385at 4041.715 kb on + strand, within BWI76_RS19385at 4041.744 kb on + strand, within BWI76_RS19385at 4041.752 kb on - strand, within BWI76_RS19385at 4042.060 kb on - strand, within BWI76_RS19385at 4042.086 kb on - strand, within BWI76_RS19385at 4042.184 kb on - strand, within BWI76_RS19385at 4042.264 kb on - strand, within BWI76_RS19385at 4042.414 kb on + strand, within BWI76_RS19385at 4042.529 kb on - strandat 4042.674 kb on - strandat 4043.242 kb on - strandat 4043.285 kb on + strandat 4043.405 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MM10
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4,039,865 + BWI76_RS19380 0.44 -0.7
4,039,906 - BWI76_RS19380 0.49 -0.1
4,040,019 + BWI76_RS19380 0.64 +0.9
4,040,225 + -0.2
4,040,298 - -0.3
4,040,406 + +0.7
4,040,496 + -0.6
4,040,496 + +0.1
4,040,504 - +2.8
4,040,504 - -0.8
4,040,514 - -0.2
4,040,867 - BWI76_RS19385 0.10 -2.7
4,041,393 - BWI76_RS19385 0.37 -1.3
4,041,588 + BWI76_RS19385 0.47 -0.3
4,041,588 + BWI76_RS19385 0.47 +1.2
4,041,715 + BWI76_RS19385 0.53 -1.1
4,041,744 + BWI76_RS19385 0.55 +0.2
4,041,752 - BWI76_RS19385 0.55 +0.7
4,042,060 - BWI76_RS19385 0.71 +0.0
4,042,086 - BWI76_RS19385 0.72 -0.3
4,042,184 - BWI76_RS19385 0.77 -0.2
4,042,264 - BWI76_RS19385 0.81 -1.1
4,042,414 + BWI76_RS19385 0.89 -0.2
4,042,529 - -1.9
4,042,674 - -0.9
4,043,242 - +0.4
4,043,285 + +0.0
4,043,405 + -0.3

Or see this region's nucleotide sequence