Experiment: Survival; Treatment=20250415-M6-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS12270 and ABCV34_RS12275 are separated by 15 nucleotides ABCV34_RS12275 and ABCV34_RS12280 overlap by 14 nucleotides ABCV34_RS12280 and ABCV34_RS12285 are separated by 160 nucleotides
ABCV34_RS12270: ABCV34_RS12270 - TRAP transporter large permease subunit, at 2,592,857 to 2,594,137
_RS12270
ABCV34_RS12275: ABCV34_RS12275 - TRAP transporter small permease, at 2,594,153 to 2,594,641
_RS12275
ABCV34_RS12280: ABCV34_RS12280 - gluconokinase, at 2,594,628 to 2,595,179
_RS12280
ABCV34_RS12285: ABCV34_RS12285 - LacI family DNA-binding transcriptional regulator, at 2,595,340 to 2,596,404
_RS12285
Position (kb)
2594
2595
2596 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.642 kb on + strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.643 kb on - strand, within ABCV34_RS12270 at 2593.659 kb on + strand, within ABCV34_RS12270 at 2593.659 kb on + strand, within ABCV34_RS12270 at 2593.659 kb on + strand, within ABCV34_RS12270 at 2593.659 kb on + strand, within ABCV34_RS12270 at 2593.660 kb on - strand, within ABCV34_RS12270 at 2593.663 kb on + strand, within ABCV34_RS12270 at 2593.663 kb on + strand, within ABCV34_RS12270 at 2593.663 kb on + strand, within ABCV34_RS12270 at 2593.663 kb on + strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.664 kb on - strand, within ABCV34_RS12270 at 2593.731 kb on + strand, within ABCV34_RS12270 at 2593.732 kb on - strand, within ABCV34_RS12270 at 2593.851 kb on + strand, within ABCV34_RS12270 at 2593.851 kb on + strand, within ABCV34_RS12270 at 2593.852 kb on - strand, within ABCV34_RS12270 at 2593.852 kb on - strand, within ABCV34_RS12270 at 2593.852 kb on - strand, within ABCV34_RS12270 at 2593.852 kb on - strand, within ABCV34_RS12270 at 2594.219 kb on + strand, within ABCV34_RS12275 at 2594.220 kb on - strand, within ABCV34_RS12275 at 2594.220 kb on - strand, within ABCV34_RS12275 at 2594.268 kb on + strand, within ABCV34_RS12275 at 2594.268 kb on + strand, within ABCV34_RS12275 at 2594.268 kb on + strand, within ABCV34_RS12275 at 2594.268 kb on + strand, within ABCV34_RS12275 at 2594.268 kb on + strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.269 kb on - strand, within ABCV34_RS12275 at 2594.312 kb on + strand, within ABCV34_RS12275 at 2594.312 kb on + strand, within ABCV34_RS12275 at 2594.312 kb on + strand, within ABCV34_RS12275 at 2594.313 kb on - strand, within ABCV34_RS12275 at 2594.425 kb on - strand, within ABCV34_RS12275 at 2594.425 kb on - strand, within ABCV34_RS12275 at 2594.450 kb on + strand, within ABCV34_RS12275 at 2594.450 kb on + strand, within ABCV34_RS12275 at 2594.450 kb on + strand, within ABCV34_RS12275 at 2594.450 kb on + strand, within ABCV34_RS12275 at 2594.450 kb on + strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.451 kb on - strand, within ABCV34_RS12275 at 2594.487 kb on + strand, within ABCV34_RS12275 at 2594.487 kb on + strand, within ABCV34_RS12275 at 2594.487 kb on + strand, within ABCV34_RS12275 at 2594.487 kb on + strand, within ABCV34_RS12275 at 2594.487 kb on + strand, within ABCV34_RS12275 at 2594.488 kb on - strand, within ABCV34_RS12275 at 2594.488 kb on - strand, within ABCV34_RS12275 at 2594.488 kb on - strand, within ABCV34_RS12275 at 2594.488 kb on - strand, within ABCV34_RS12275 at 2594.514 kb on + strand, within ABCV34_RS12275 at 2594.515 kb on - strand, within ABCV34_RS12275 at 2594.515 kb on - strand, within ABCV34_RS12275 at 2594.515 kb on - strand, within ABCV34_RS12275 at 2594.887 kb on + strand, within ABCV34_RS12280 at 2594.888 kb on - strand, within ABCV34_RS12280 at 2594.888 kb on - strand, within ABCV34_RS12280 at 2594.888 kb on - strand, within ABCV34_RS12280 at 2594.888 kb on - strand, within ABCV34_RS12280 at 2594.955 kb on + strand, within ABCV34_RS12280 at 2594.956 kb on - strand, within ABCV34_RS12280 at 2594.956 kb on - strand, within ABCV34_RS12280 at 2595.183 kb on + strand at 2595.183 kb on + strand at 2595.184 kb on - strand at 2595.226 kb on + strand at 2595.227 kb on - strand at 2595.235 kb on + strand at 2595.235 kb on + strand at 2595.235 kb on + strand at 2595.235 kb on + strand at 2595.236 kb on - strand at 2595.300 kb on + strand at 2595.300 kb on + strand at 2595.301 kb on - strand at 2595.306 kb on + strand at 2595.307 kb on - strand at 2595.326 kb on + strand at 2595.339 kb on + strand at 2595.340 kb on - strand at 2595.340 kb on - strand at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.627 kb on + strand, within ABCV34_RS12285 at 2595.709 kb on + strand, within ABCV34_RS12285 at 2595.709 kb on + strand, within ABCV34_RS12285 at 2595.710 kb on - strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.745 kb on + strand, within ABCV34_RS12285 at 2595.746 kb on - strand, within ABCV34_RS12285 at 2595.746 kb on - strand, within ABCV34_RS12285 at 2595.746 kb on - strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.872 kb on + strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2595.873 kb on - strand, within ABCV34_RS12285 at 2596.046 kb on + strand, within ABCV34_RS12285 at 2596.047 kb on - strand, within ABCV34_RS12285 at 2596.146 kb on + strand, within ABCV34_RS12285 at 2596.147 kb on - strand, within ABCV34_RS12285 at 2596.147 kb on - strand, within ABCV34_RS12285
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-M6-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 2,593,642 + ABCV34_RS12270 0.61 -1.2 2,593,642 + ABCV34_RS12270 0.61 -2.5 2,593,642 + ABCV34_RS12270 0.61 -1.9 2,593,642 + ABCV34_RS12270 0.61 -3.8 2,593,642 + ABCV34_RS12270 0.61 +1.2 2,593,642 + ABCV34_RS12270 0.61 +0.3 2,593,642 + ABCV34_RS12270 0.61 +0.3 2,593,642 + ABCV34_RS12270 0.61 -0.5 2,593,642 + ABCV34_RS12270 0.61 -0.9 2,593,642 + ABCV34_RS12270 0.61 -0.5 2,593,642 + ABCV34_RS12270 0.61 +0.6 2,593,642 + ABCV34_RS12270 0.61 +0.1 2,593,642 + ABCV34_RS12270 0.61 -3.1 2,593,642 + ABCV34_RS12270 0.61 -0.6 2,593,643 - ABCV34_RS12270 0.61 -1.8 2,593,643 - ABCV34_RS12270 0.61 -0.8 2,593,643 - ABCV34_RS12270 0.61 +0.8 2,593,643 - ABCV34_RS12270 0.61 -3.2 2,593,643 - ABCV34_RS12270 0.61 -1.2 2,593,643 - ABCV34_RS12270 0.61 +0.1 2,593,643 - ABCV34_RS12270 0.61 -1.4 2,593,643 - ABCV34_RS12270 0.61 +1.1 2,593,643 - ABCV34_RS12270 0.61 +0.6 2,593,643 - ABCV34_RS12270 0.61 -1.5 2,593,643 - ABCV34_RS12270 0.61 +1.2 2,593,643 - ABCV34_RS12270 0.61 -2.3 2,593,643 - ABCV34_RS12270 0.61 -1.8 2,593,643 - ABCV34_RS12270 0.61 -3.6 2,593,643 - ABCV34_RS12270 0.61 -0.3 2,593,659 + ABCV34_RS12270 0.63 +1.6 2,593,659 + ABCV34_RS12270 0.63 -1.2 2,593,659 + ABCV34_RS12270 0.63 -2.3 2,593,659 + ABCV34_RS12270 0.63 -2.0 2,593,660 - ABCV34_RS12270 0.63 -1.4 2,593,663 + ABCV34_RS12270 0.63 -2.2 2,593,663 + ABCV34_RS12270 0.63 -1.4 2,593,663 + ABCV34_RS12270 0.63 -3.2 2,593,663 + ABCV34_RS12270 0.63 -2.4 2,593,664 - ABCV34_RS12270 0.63 -2.2 2,593,664 - ABCV34_RS12270 0.63 -1.7 2,593,664 - ABCV34_RS12270 0.63 -1.0 2,593,664 - ABCV34_RS12270 0.63 -0.0 2,593,664 - ABCV34_RS12270 0.63 -0.8 2,593,664 - ABCV34_RS12270 0.63 +0.6 2,593,664 - ABCV34_RS12270 0.63 -0.6 2,593,731 + ABCV34_RS12270 0.68 -1.5 2,593,732 - ABCV34_RS12270 0.68 -1.5 2,593,851 + ABCV34_RS12270 0.78 -2.7 2,593,851 + ABCV34_RS12270 0.78 -0.1 2,593,852 - ABCV34_RS12270 0.78 -2.2 2,593,852 - ABCV34_RS12270 0.78 -1.2 2,593,852 - ABCV34_RS12270 0.78 -1.0 2,593,852 - ABCV34_RS12270 0.78 -1.8 2,594,219 + ABCV34_RS12275 0.13 -1.1 2,594,220 - ABCV34_RS12275 0.14 -1.7 2,594,220 - ABCV34_RS12275 0.14 -1.6 2,594,268 + ABCV34_RS12275 0.24 +0.9 2,594,268 + ABCV34_RS12275 0.24 -0.6 2,594,268 + ABCV34_RS12275 0.24 -1.0 2,594,268 + ABCV34_RS12275 0.24 -2.9 2,594,268 + ABCV34_RS12275 0.24 -0.2 2,594,269 - ABCV34_RS12275 0.24 +0.4 2,594,269 - ABCV34_RS12275 0.24 -1.2 2,594,269 - ABCV34_RS12275 0.24 -1.6 2,594,269 - ABCV34_RS12275 0.24 +0.8 2,594,269 - ABCV34_RS12275 0.24 -2.6 2,594,269 - ABCV34_RS12275 0.24 -4.0 2,594,269 - ABCV34_RS12275 0.24 -1.0 2,594,312 + ABCV34_RS12275 0.33 -0.8 2,594,312 + ABCV34_RS12275 0.33 -0.5 2,594,312 + ABCV34_RS12275 0.33 -0.6 2,594,313 - ABCV34_RS12275 0.33 -3.0 2,594,425 - ABCV34_RS12275 0.56 -0.9 2,594,425 - ABCV34_RS12275 0.56 -1.9 2,594,450 + ABCV34_RS12275 0.61 -1.0 2,594,450 + ABCV34_RS12275 0.61 -0.9 2,594,450 + ABCV34_RS12275 0.61 -1.5 2,594,450 + ABCV34_RS12275 0.61 -1.7 2,594,450 + ABCV34_RS12275 0.61 -1.4 2,594,451 - ABCV34_RS12275 0.61 -2.0 2,594,451 - ABCV34_RS12275 0.61 +0.6 2,594,451 - ABCV34_RS12275 0.61 -0.9 2,594,451 - ABCV34_RS12275 0.61 -0.6 2,594,451 - ABCV34_RS12275 0.61 -2.0 2,594,451 - ABCV34_RS12275 0.61 -2.6 2,594,451 - ABCV34_RS12275 0.61 -2.4 2,594,451 - ABCV34_RS12275 0.61 -1.5 2,594,451 - ABCV34_RS12275 0.61 -0.7 2,594,487 + ABCV34_RS12275 0.68 -1.8 2,594,487 + ABCV34_RS12275 0.68 -0.9 2,594,487 + ABCV34_RS12275 0.68 -2.4 2,594,487 + ABCV34_RS12275 0.68 -0.8 2,594,487 + ABCV34_RS12275 0.68 +1.6 2,594,488 - ABCV34_RS12275 0.69 -2.2 2,594,488 - ABCV34_RS12275 0.69 -0.2 2,594,488 - ABCV34_RS12275 0.69 -0.9 2,594,488 - ABCV34_RS12275 0.69 -0.0 2,594,514 + ABCV34_RS12275 0.74 -1.1 2,594,515 - ABCV34_RS12275 0.74 -0.5 2,594,515 - ABCV34_RS12275 0.74 -1.4 2,594,515 - ABCV34_RS12275 0.74 -0.5 2,594,887 + ABCV34_RS12280 0.47 +1.0 2,594,888 - ABCV34_RS12280 0.47 -1.9 2,594,888 - ABCV34_RS12280 0.47 -3.0 2,594,888 - ABCV34_RS12280 0.47 +0.3 2,594,888 - ABCV34_RS12280 0.47 -2.0 2,594,955 + ABCV34_RS12280 0.59 +0.6 2,594,956 - ABCV34_RS12280 0.59 -1.9 2,594,956 - ABCV34_RS12280 0.59 +1.8 2,595,183 + -3.0 2,595,183 + -1.8 2,595,184 - -0.2 2,595,226 + +1.4 2,595,227 - -0.3 2,595,235 + -3.6 2,595,235 + -3.2 2,595,235 + -0.5 2,595,235 + +0.2 2,595,236 - -1.1 2,595,300 + -1.0 2,595,300 + -3.0 2,595,301 - -1.8 2,595,306 + +0.2 2,595,307 - -1.5 2,595,326 + -1.0 2,595,339 + -3.1 2,595,340 - -1.5 2,595,340 - -1.4 2,595,627 + ABCV34_RS12285 0.27 -0.9 2,595,627 + ABCV34_RS12285 0.27 -3.3 2,595,627 + ABCV34_RS12285 0.27 -0.4 2,595,627 + ABCV34_RS12285 0.27 +0.2 2,595,627 + ABCV34_RS12285 0.27 +1.1 2,595,627 + ABCV34_RS12285 0.27 -1.0 2,595,709 + ABCV34_RS12285 0.35 -0.9 2,595,709 + ABCV34_RS12285 0.35 -1.2 2,595,710 - ABCV34_RS12285 0.35 -2.5 2,595,745 + ABCV34_RS12285 0.38 -0.2 2,595,745 + ABCV34_RS12285 0.38 -1.5 2,595,745 + ABCV34_RS12285 0.38 -1.7 2,595,745 + ABCV34_RS12285 0.38 -1.4 2,595,745 + ABCV34_RS12285 0.38 -2.0 2,595,745 + ABCV34_RS12285 0.38 -1.5 2,595,746 - ABCV34_RS12285 0.38 -1.7 2,595,746 - ABCV34_RS12285 0.38 +0.4 2,595,746 - ABCV34_RS12285 0.38 -0.4 2,595,872 + ABCV34_RS12285 0.50 -0.3 2,595,872 + ABCV34_RS12285 0.50 -1.3 2,595,872 + ABCV34_RS12285 0.50 -1.7 2,595,872 + ABCV34_RS12285 0.50 -2.8 2,595,872 + ABCV34_RS12285 0.50 -0.3 2,595,872 + ABCV34_RS12285 0.50 -1.0 2,595,872 + ABCV34_RS12285 0.50 -1.4 2,595,872 + ABCV34_RS12285 0.50 -0.5 2,595,872 + ABCV34_RS12285 0.50 -1.8 2,595,872 + ABCV34_RS12285 0.50 -0.7 2,595,873 - ABCV34_RS12285 0.50 -0.1 2,595,873 - ABCV34_RS12285 0.50 -0.9 2,595,873 - ABCV34_RS12285 0.50 +0.8 2,595,873 - ABCV34_RS12285 0.50 -0.7 2,595,873 - ABCV34_RS12285 0.50 -2.0 2,595,873 - ABCV34_RS12285 0.50 -1.7 2,595,873 - ABCV34_RS12285 0.50 -2.1 2,595,873 - ABCV34_RS12285 0.50 +0.3 2,595,873 - ABCV34_RS12285 0.50 -0.1 2,596,046 + ABCV34_RS12285 0.66 +1.0 2,596,047 - ABCV34_RS12285 0.66 -0.7 2,596,146 + ABCV34_RS12285 0.76 -1.8 2,596,147 - ABCV34_RS12285 0.76 -0.1 2,596,147 - ABCV34_RS12285 0.76 -0.5
Or see this region's nucleotide sequence