Strain Fitness in Castellaniella sp019104865 MT123 around ABCV34_RS15290

Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABCV34_RS15280 and ABCV34_RS15285 are separated by 190 nucleotidesABCV34_RS15285 and ABCV34_RS15290 overlap by 11 nucleotidesABCV34_RS15290 and ABCV34_RS15295 overlap by 1 nucleotides ABCV34_RS15280: ABCV34_RS15280 - biotin synthase BioB, at 3,239,578 to 3,240,573 _RS15280 ABCV34_RS15285: ABCV34_RS15285 - dethiobiotin synthase, at 3,240,764 to 3,241,462 _RS15285 ABCV34_RS15290: ABCV34_RS15290 - methyltransferase domain-containing protein, at 3,241,452 to 3,242,297 _RS15290 ABCV34_RS15295: ABCV34_RS15295 - 8-amino-7-oxononanoate synthase, at 3,242,297 to 3,243,472 _RS15295 Position (kb) 3241 3242 3243Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3at 3240.611 kb on + strandat 3240.614 kb on + strandat 3240.615 kb on - strandat 3240.615 kb on - strandat 3240.615 kb on - strandat 3240.669 kb on + strandat 3240.678 kb on - strandat 3240.678 kb on - strandat 3240.761 kb on + strandat 3240.761 kb on + strandat 3240.761 kb on + strandat 3240.761 kb on + strandat 3240.762 kb on - strandat 3240.762 kb on - strandat 3240.762 kb on - strandat 3240.762 kb on - strandat 3240.797 kb on + strandat 3240.798 kb on - strandat 3241.040 kb on - strand, within ABCV34_RS15285at 3241.064 kb on + strand, within ABCV34_RS15285at 3241.064 kb on + strand, within ABCV34_RS15285at 3241.064 kb on + strand, within ABCV34_RS15285at 3241.065 kb on - strand, within ABCV34_RS15285at 3241.065 kb on - strand, within ABCV34_RS15285at 3241.258 kb on - strand, within ABCV34_RS15285at 3241.345 kb on - strand, within ABCV34_RS15285at 3241.535 kb on - strandat 3241.971 kb on + strand, within ABCV34_RS15290at 3242.210 kb on - strand, within ABCV34_RS15290at 3242.694 kb on + strand, within ABCV34_RS15295at 3242.694 kb on + strand, within ABCV34_RS15295at 3242.694 kb on + strand, within ABCV34_RS15295at 3242.850 kb on + strand, within ABCV34_RS15295at 3242.850 kb on + strand, within ABCV34_RS15295at 3242.851 kb on - strand, within ABCV34_RS15295at 3242.851 kb on - strand, within ABCV34_RS15295at 3243.004 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.081 kb on + strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.082 kb on - strand, within ABCV34_RS15295at 3243.276 kb on + strand, within ABCV34_RS15295

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Per-strain Table

Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
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3,240,611 + +2.9
3,240,614 + -3.3
3,240,615 - +2.6
3,240,615 - -3.6
3,240,615 - -2.5
3,240,669 + -5.2
3,240,678 - +0.3
3,240,678 - +1.9
3,240,761 + +3.5
3,240,761 + -0.4
3,240,761 + +2.5
3,240,761 + +0.5
3,240,762 - -3.5
3,240,762 - -2.8
3,240,762 - -0.7
3,240,762 - -2.5
3,240,797 + -1.4
3,240,798 - -3.9
3,241,040 - ABCV34_RS15285 0.39 -3.4
3,241,064 + ABCV34_RS15285 0.43 -0.6
3,241,064 + ABCV34_RS15285 0.43 -0.7
3,241,064 + ABCV34_RS15285 0.43 -0.3
3,241,065 - ABCV34_RS15285 0.43 -0.3
3,241,065 - ABCV34_RS15285 0.43 -2.9
3,241,258 - ABCV34_RS15285 0.71 -0.9
3,241,345 - ABCV34_RS15285 0.83 -0.6
3,241,535 - +1.0
3,241,971 + ABCV34_RS15290 0.61 +0.6
3,242,210 - ABCV34_RS15290 0.90 +0.3
3,242,694 + ABCV34_RS15295 0.34 -3.4
3,242,694 + ABCV34_RS15295 0.34 +0.3
3,242,694 + ABCV34_RS15295 0.34 -3.1
3,242,850 + ABCV34_RS15295 0.47 -0.6
3,242,850 + ABCV34_RS15295 0.47 +0.8
3,242,851 - ABCV34_RS15295 0.47 -1.5
3,242,851 - ABCV34_RS15295 0.47 -0.8
3,243,004 + ABCV34_RS15295 0.60 -0.2
3,243,081 + ABCV34_RS15295 0.67 -2.5
3,243,081 + ABCV34_RS15295 0.67 +0.3
3,243,081 + ABCV34_RS15295 0.67 +1.9
3,243,081 + ABCV34_RS15295 0.67 +0.8
3,243,081 + ABCV34_RS15295 0.67 -4.6
3,243,081 + ABCV34_RS15295 0.67 -2.3
3,243,081 + ABCV34_RS15295 0.67 -2.9
3,243,081 + ABCV34_RS15295 0.67 -1.3
3,243,081 + ABCV34_RS15295 0.67 -1.2
3,243,081 + ABCV34_RS15295 0.67 +0.2
3,243,082 - ABCV34_RS15295 0.67 -2.8
3,243,082 - ABCV34_RS15295 0.67 -1.5
3,243,082 - ABCV34_RS15295 0.67 -0.6
3,243,082 - ABCV34_RS15295 0.67 +1.1
3,243,082 - ABCV34_RS15295 0.67 -1.8
3,243,082 - ABCV34_RS15295 0.67 -3.9
3,243,082 - ABCV34_RS15295 0.67 -2.7
3,243,082 - ABCV34_RS15295 0.67 -1.1
3,243,082 - ABCV34_RS15295 0.67 -3.7
3,243,082 - ABCV34_RS15295 0.67 -2.7
3,243,082 - ABCV34_RS15295 0.67 -1.7
3,243,082 - ABCV34_RS15295 0.67 +1.1
3,243,276 + ABCV34_RS15295 0.83 -5.8

Or see this region's nucleotide sequence