Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS13705 and ABCV34_RS13710 are separated by 37 nucleotides ABCV34_RS13710 and ABCV34_RS13715 are separated by 4 nucleotides ABCV34_RS13715 and ABCV34_RS13720 are separated by 20 nucleotides
ABCV34_RS13705: ABCV34_RS13705 - phenylacetate--CoA ligase PaaK, at 2,904,188 to 2,905,510
_RS13705
ABCV34_RS13710: ABCV34_RS13710 - hydroxyphenylacetyl-CoA thioesterase PaaI, at 2,905,548 to 2,906,015
_RS13710
ABCV34_RS13715: ABCV34_RS13715 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG, at 2,906,020 to 2,906,817
_RS13715
ABCV34_RS13720: ABCV34_RS13720 - phenylacetic acid degradation protein PaaN, at 2,906,838 to 2,908,505
_RS13720
Position (kb)
2905
2906
2907 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2904.562 kb on + strand, within ABCV34_RS13705 at 2904.563 kb on - strand, within ABCV34_RS13705 at 2904.563 kb on - strand, within ABCV34_RS13705 at 2904.702 kb on + strand, within ABCV34_RS13705 at 2904.702 kb on + strand, within ABCV34_RS13705 at 2904.789 kb on + strand, within ABCV34_RS13705 at 2904.789 kb on + strand, within ABCV34_RS13705 at 2904.789 kb on + strand, within ABCV34_RS13705 at 2904.790 kb on - strand, within ABCV34_RS13705 at 2904.790 kb on - strand, within ABCV34_RS13705 at 2904.886 kb on + strand, within ABCV34_RS13705 at 2904.886 kb on + strand, within ABCV34_RS13705 at 2904.886 kb on + strand, within ABCV34_RS13705 at 2904.886 kb on + strand, within ABCV34_RS13705 at 2904.887 kb on - strand, within ABCV34_RS13705 at 2904.939 kb on + strand, within ABCV34_RS13705 at 2904.939 kb on + strand, within ABCV34_RS13705 at 2904.939 kb on + strand, within ABCV34_RS13705 at 2904.940 kb on - strand, within ABCV34_RS13705 at 2905.030 kb on + strand, within ABCV34_RS13705 at 2905.030 kb on + strand, within ABCV34_RS13705 at 2905.030 kb on + strand, within ABCV34_RS13705 at 2905.031 kb on - strand, within ABCV34_RS13705 at 2905.275 kb on + strand, within ABCV34_RS13705 at 2905.276 kb on - strand, within ABCV34_RS13705 at 2905.276 kb on - strand, within ABCV34_RS13705 at 2905.381 kb on - strand at 2905.398 kb on + strand at 2905.398 kb on + strand at 2905.399 kb on - strand at 2905.399 kb on - strand at 2905.399 kb on - strand at 2905.399 kb on - strand at 2905.399 kb on - strand at 2905.530 kb on + strand at 2905.530 kb on + strand at 2905.530 kb on + strand at 2905.530 kb on + strand at 2905.530 kb on + strand at 2905.531 kb on - strand at 2905.653 kb on - strand, within ABCV34_RS13710 at 2905.669 kb on + strand, within ABCV34_RS13710 at 2905.670 kb on - strand, within ABCV34_RS13710 at 2905.670 kb on - strand, within ABCV34_RS13710 at 2905.670 kb on - strand, within ABCV34_RS13710 at 2905.670 kb on - strand, within ABCV34_RS13710 at 2905.801 kb on + strand, within ABCV34_RS13710 at 2905.802 kb on - strand, within ABCV34_RS13710 at 2906.078 kb on + strand at 2906.546 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.721 kb on + strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.722 kb on - strand, within ABCV34_RS13715 at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.899 kb on + strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.900 kb on - strand at 2906.947 kb on + strand at 2906.968 kb on + strand at 2906.968 kb on + strand at 2906.968 kb on + strand at 2906.968 kb on + strand at 2906.968 kb on + strand at 2906.968 kb on + strand at 2906.969 kb on - strand at 2906.969 kb on - strand at 2906.969 kb on - strand at 2906.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 2,904,562 + ABCV34_RS13705 0.28 -0.3 2,904,563 - ABCV34_RS13705 0.28 -1.5 2,904,563 - ABCV34_RS13705 0.28 -1.8 2,904,702 + ABCV34_RS13705 0.39 -2.6 2,904,702 + ABCV34_RS13705 0.39 -1.2 2,904,789 + ABCV34_RS13705 0.45 -1.6 2,904,789 + ABCV34_RS13705 0.45 -3.3 2,904,789 + ABCV34_RS13705 0.45 -1.5 2,904,790 - ABCV34_RS13705 0.46 -3.5 2,904,790 - ABCV34_RS13705 0.46 -3.3 2,904,886 + ABCV34_RS13705 0.53 -1.1 2,904,886 + ABCV34_RS13705 0.53 -3.5 2,904,886 + ABCV34_RS13705 0.53 +1.4 2,904,886 + ABCV34_RS13705 0.53 -1.9 2,904,887 - ABCV34_RS13705 0.53 +1.4 2,904,939 + ABCV34_RS13705 0.57 +1.4 2,904,939 + ABCV34_RS13705 0.57 -1.1 2,904,939 + ABCV34_RS13705 0.57 -0.2 2,904,940 - ABCV34_RS13705 0.57 -1.6 2,905,030 + ABCV34_RS13705 0.64 +0.8 2,905,030 + ABCV34_RS13705 0.64 -3.1 2,905,030 + ABCV34_RS13705 0.64 -2.3 2,905,031 - ABCV34_RS13705 0.64 +0.3 2,905,275 + ABCV34_RS13705 0.82 -3.8 2,905,276 - ABCV34_RS13705 0.82 -0.2 2,905,276 - ABCV34_RS13705 0.82 -4.4 2,905,381 - -1.4 2,905,398 + -0.2 2,905,398 + +0.0 2,905,399 - -3.4 2,905,399 - -1.1 2,905,399 - -2.2 2,905,399 - -2.8 2,905,399 - -3.2 2,905,530 + -0.7 2,905,530 + -0.1 2,905,530 + -3.5 2,905,530 + -3.2 2,905,530 + -3.0 2,905,531 - +0.3 2,905,653 - ABCV34_RS13710 0.22 -1.4 2,905,669 + ABCV34_RS13710 0.26 -2.6 2,905,670 - ABCV34_RS13710 0.26 -2.1 2,905,670 - ABCV34_RS13710 0.26 -1.2 2,905,670 - ABCV34_RS13710 0.26 +0.5 2,905,670 - ABCV34_RS13710 0.26 +0.2 2,905,801 + ABCV34_RS13710 0.54 +2.5 2,905,802 - ABCV34_RS13710 0.54 -3.8 2,906,078 + -0.1 2,906,546 + ABCV34_RS13715 0.66 -3.1 2,906,721 + ABCV34_RS13715 0.88 -0.7 2,906,721 + ABCV34_RS13715 0.88 -0.4 2,906,721 + ABCV34_RS13715 0.88 -0.1 2,906,721 + ABCV34_RS13715 0.88 +0.9 2,906,721 + ABCV34_RS13715 0.88 -5.3 2,906,721 + ABCV34_RS13715 0.88 -1.0 2,906,721 + ABCV34_RS13715 0.88 +1.5 2,906,721 + ABCV34_RS13715 0.88 +2.4 2,906,721 + ABCV34_RS13715 0.88 -1.2 2,906,721 + ABCV34_RS13715 0.88 -3.3 2,906,722 - ABCV34_RS13715 0.88 +0.6 2,906,722 - ABCV34_RS13715 0.88 -1.7 2,906,722 - ABCV34_RS13715 0.88 -1.2 2,906,722 - ABCV34_RS13715 0.88 -4.4 2,906,722 - ABCV34_RS13715 0.88 -1.2 2,906,722 - ABCV34_RS13715 0.88 +0.2 2,906,899 + -0.1 2,906,899 + -1.4 2,906,899 + +0.1 2,906,899 + -3.2 2,906,899 + -3.9 2,906,899 + -0.0 2,906,899 + -1.1 2,906,899 + -0.8 2,906,899 + +0.4 2,906,900 - -0.8 2,906,900 - -2.0 2,906,900 - -2.8 2,906,900 - -0.8 2,906,900 - -2.2 2,906,900 - +2.3 2,906,900 - -1.3 2,906,900 - -1.1 2,906,900 - -0.5 2,906,947 + -0.3 2,906,968 + +0.1 2,906,968 + -0.2 2,906,968 + +3.0 2,906,968 + -0.5 2,906,968 + -1.4 2,906,968 + -2.5 2,906,969 - -3.4 2,906,969 - -3.0 2,906,969 - -1.2 2,906,969 - -4.0
Or see this region's nucleotide sequence