Strain Fitness in Castellaniella sp019104865 MT123 around ABCV34_RS04335

Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABCV34_RS04325 and ABCV34_RS04330 overlap by 17 nucleotidesABCV34_RS04330 and ABCV34_RS04335 are separated by 68 nucleotidesABCV34_RS04335 and ABCV34_RS04340 are separated by 100 nucleotides ABCV34_RS04325: ABCV34_RS04325 - YihY family inner membrane protein, at 944,409 to 945,716 _RS04325 ABCV34_RS04330: ABCV34_RS04330 - CBS domain-containing protein, at 945,700 to 946,167 _RS04330 ABCV34_RS04335: ABCV34_RS04335 - tRNA dihydrouridine(20/20a) synthase DusA, at 946,236 to 947,240 _RS04335 ABCV34_RS04340: ABCV34_RS04340 - homoserine kinase, at 947,341 to 948,315 _RS04340 Position (kb) 946 947 948Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 945.344 kb on - strand, within ABCV34_RS04325at 945.438 kb on + strand, within ABCV34_RS04325at 945.439 kb on - strand, within ABCV34_RS04325at 945.439 kb on - strand, within ABCV34_RS04325at 945.586 kb on + strandat 945.660 kb on + strandat 945.660 kb on + strandat 945.661 kb on - strandat 945.661 kb on - strandat 945.661 kb on - strandat 945.745 kb on + strandat 945.746 kb on - strandat 945.746 kb on - strandat 945.746 kb on - strandat 945.746 kb on - strandat 945.763 kb on - strand, within ABCV34_RS04330at 945.907 kb on + strand, within ABCV34_RS04330at 945.907 kb on + strand, within ABCV34_RS04330at 945.907 kb on + strand, within ABCV34_RS04330at 945.907 kb on + strand, within ABCV34_RS04330at 945.908 kb on - strand, within ABCV34_RS04330at 945.908 kb on - strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.006 kb on + strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.007 kb on - strand, within ABCV34_RS04330at 946.054 kb on + strand, within ABCV34_RS04330at 946.054 kb on + strand, within ABCV34_RS04330at 946.054 kb on + strand, within ABCV34_RS04330at 946.054 kb on + strand, within ABCV34_RS04330at 946.054 kb on + strand, within ABCV34_RS04330at 946.055 kb on - strand, within ABCV34_RS04330at 946.055 kb on - strand, within ABCV34_RS04330at 946.055 kb on - strand, within ABCV34_RS04330at 946.055 kb on - strand, within ABCV34_RS04330at 946.055 kb on - strand, within ABCV34_RS04330at 946.102 kb on - strand, within ABCV34_RS04330at 946.102 kb on - strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.114 kb on + strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.115 kb on - strand, within ABCV34_RS04330at 946.735 kb on - strand, within ABCV34_RS04335at 946.753 kb on + strand, within ABCV34_RS04335at 946.753 kb on + strand, within ABCV34_RS04335at 946.753 kb on + strand, within ABCV34_RS04335at 946.753 kb on + strand, within ABCV34_RS04335at 946.753 kb on + strand, within ABCV34_RS04335at 946.754 kb on - strand, within ABCV34_RS04335at 946.754 kb on - strand, within ABCV34_RS04335at 946.754 kb on - strand, within ABCV34_RS04335at 946.754 kb on - strand, within ABCV34_RS04335at 946.787 kb on - strand, within ABCV34_RS04335at 946.787 kb on - strand, within ABCV34_RS04335at 946.798 kb on + strand, within ABCV34_RS04335at 946.799 kb on - strand, within ABCV34_RS04335at 947.116 kb on + strand, within ABCV34_RS04335at 947.116 kb on + strand, within ABCV34_RS04335at 947.116 kb on + strand, within ABCV34_RS04335at 947.116 kb on + strand, within ABCV34_RS04335at 947.116 kb on + strand, within ABCV34_RS04335at 947.152 kb on + strandat 947.152 kb on + strandat 947.153 kb on - strandat 947.153 kb on - strandat 947.153 kb on - strandat 947.225 kb on - strandat 947.252 kb on + strandat 947.253 kb on - strandat 947.253 kb on - strandat 947.269 kb on + strandat 947.274 kb on + strandat 947.275 kb on - strandat 947.285 kb on - strandat 947.334 kb on - strandat 947.334 kb on - strandat 947.349 kb on - strandat 947.395 kb on + strandat 947.455 kb on + strand, within ABCV34_RS04340at 947.482 kb on + strand, within ABCV34_RS04340at 947.483 kb on - strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.530 kb on + strand, within ABCV34_RS04340at 947.531 kb on - strand, within ABCV34_RS04340at 947.531 kb on - strand, within ABCV34_RS04340at 947.532 kb on + strand, within ABCV34_RS04340at 947.662 kb on + strand, within ABCV34_RS04340at 947.801 kb on + strand, within ABCV34_RS04340at 947.801 kb on + strand, within ABCV34_RS04340at 947.801 kb on + strand, within ABCV34_RS04340at 947.801 kb on + strand, within ABCV34_RS04340at 947.801 kb on + strand, within ABCV34_RS04340at 947.802 kb on - strand, within ABCV34_RS04340at 947.802 kb on - strand, within ABCV34_RS04340at 947.802 kb on - strand, within ABCV34_RS04340at 947.802 kb on - strand, within ABCV34_RS04340at 947.900 kb on - strand, within ABCV34_RS04340at 947.989 kb on + strand, within ABCV34_RS04340at 947.989 kb on + strand, within ABCV34_RS04340at 947.990 kb on - strand, within ABCV34_RS04340at 948.048 kb on + strand, within ABCV34_RS04340at 948.049 kb on - strand, within ABCV34_RS04340at 948.107 kb on - strand, within ABCV34_RS04340at 948.208 kb on + strand, within ABCV34_RS04340

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Per-strain Table

Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
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945,344 - ABCV34_RS04325 0.71 -0.3
945,438 + ABCV34_RS04325 0.79 +0.4
945,439 - ABCV34_RS04325 0.79 -5.0
945,439 - ABCV34_RS04325 0.79 -4.1
945,586 + -2.5
945,660 + -2.9
945,660 + -0.6
945,661 - -2.2
945,661 - -1.9
945,661 - +0.1
945,745 + -1.3
945,746 - -2.3
945,746 - +0.6
945,746 - -0.7
945,746 - -3.3
945,763 - ABCV34_RS04330 0.13 -2.2
945,907 + ABCV34_RS04330 0.44 -4.1
945,907 + ABCV34_RS04330 0.44 -0.3
945,907 + ABCV34_RS04330 0.44 -1.4
945,907 + ABCV34_RS04330 0.44 +0.3
945,908 - ABCV34_RS04330 0.44 -4.2
945,908 - ABCV34_RS04330 0.44 +0.2
946,006 + ABCV34_RS04330 0.65 -2.6
946,006 + ABCV34_RS04330 0.65 -0.1
946,006 + ABCV34_RS04330 0.65 -3.2
946,006 + ABCV34_RS04330 0.65 -1.9
946,006 + ABCV34_RS04330 0.65 -0.2
946,006 + ABCV34_RS04330 0.65 -3.7
946,006 + ABCV34_RS04330 0.65 -0.1
946,006 + ABCV34_RS04330 0.65 -4.3
946,006 + ABCV34_RS04330 0.65 -3.5
946,006 + ABCV34_RS04330 0.65 -1.1
946,007 - ABCV34_RS04330 0.66 +0.3
946,007 - ABCV34_RS04330 0.66 -1.7
946,007 - ABCV34_RS04330 0.66 -2.4
946,007 - ABCV34_RS04330 0.66 -5.1
946,007 - ABCV34_RS04330 0.66 -0.1
946,007 - ABCV34_RS04330 0.66 -3.1
946,007 - ABCV34_RS04330 0.66 -1.2
946,054 + ABCV34_RS04330 0.76 -1.1
946,054 + ABCV34_RS04330 0.76 -0.8
946,054 + ABCV34_RS04330 0.76 +0.6
946,054 + ABCV34_RS04330 0.76 -2.0
946,054 + ABCV34_RS04330 0.76 +0.7
946,055 - ABCV34_RS04330 0.76 -2.1
946,055 - ABCV34_RS04330 0.76 -0.3
946,055 - ABCV34_RS04330 0.76 +0.5
946,055 - ABCV34_RS04330 0.76 +1.1
946,055 - ABCV34_RS04330 0.76 -0.9
946,102 - ABCV34_RS04330 0.86 -1.2
946,102 - ABCV34_RS04330 0.86 -1.4
946,114 + ABCV34_RS04330 0.88 -0.7
946,114 + ABCV34_RS04330 0.88 -3.3
946,114 + ABCV34_RS04330 0.88 -3.6
946,114 + ABCV34_RS04330 0.88 -0.3
946,114 + ABCV34_RS04330 0.88 -0.5
946,114 + ABCV34_RS04330 0.88 -0.7
946,114 + ABCV34_RS04330 0.88 -4.5
946,114 + ABCV34_RS04330 0.88 +0.1
946,114 + ABCV34_RS04330 0.88 +0.9
946,114 + ABCV34_RS04330 0.88 -2.6
946,114 + ABCV34_RS04330 0.88 -1.2
946,115 - ABCV34_RS04330 0.89 +1.4
946,115 - ABCV34_RS04330 0.89 +1.4
946,115 - ABCV34_RS04330 0.89 -1.3
946,115 - ABCV34_RS04330 0.89 -1.2
946,115 - ABCV34_RS04330 0.89 -1.5
946,115 - ABCV34_RS04330 0.89 -1.6
946,115 - ABCV34_RS04330 0.89 -0.6
946,115 - ABCV34_RS04330 0.89 -4.2
946,115 - ABCV34_RS04330 0.89 -2.9
946,735 - ABCV34_RS04335 0.50 +1.4
946,753 + ABCV34_RS04335 0.51 -4.6
946,753 + ABCV34_RS04335 0.51 +0.4
946,753 + ABCV34_RS04335 0.51 -2.0
946,753 + ABCV34_RS04335 0.51 -3.6
946,753 + ABCV34_RS04335 0.51 -3.8
946,754 - ABCV34_RS04335 0.52 +0.4
946,754 - ABCV34_RS04335 0.52 +0.4
946,754 - ABCV34_RS04335 0.52 +1.0
946,754 - ABCV34_RS04335 0.52 -3.6
946,787 - ABCV34_RS04335 0.55 -3.3
946,787 - ABCV34_RS04335 0.55 -0.1
946,798 + ABCV34_RS04335 0.56 -1.5
946,799 - ABCV34_RS04335 0.56 +0.8
947,116 + ABCV34_RS04335 0.88 +0.9
947,116 + ABCV34_RS04335 0.88 -2.6
947,116 + ABCV34_RS04335 0.88 +2.6
947,116 + ABCV34_RS04335 0.88 -1.5
947,116 + ABCV34_RS04335 0.88 -4.8
947,152 + -0.4
947,152 + -0.1
947,153 - -0.8
947,153 - -3.9
947,153 - -0.9
947,225 - +0.4
947,252 + -1.9
947,253 - +0.7
947,253 - +0.0
947,269 + -2.7
947,274 + +1.0
947,275 - -3.3
947,285 - -1.1
947,334 - +5.3
947,334 - -1.2
947,349 - +0.9
947,395 + -0.1
947,455 + ABCV34_RS04340 0.12 -1.8
947,482 + ABCV34_RS04340 0.14 -0.1
947,483 - ABCV34_RS04340 0.15 +0.9
947,530 + ABCV34_RS04340 0.19 +2.5
947,530 + ABCV34_RS04340 0.19 +0.4
947,530 + ABCV34_RS04340 0.19 -2.1
947,530 + ABCV34_RS04340 0.19 -0.9
947,530 + ABCV34_RS04340 0.19 -0.9
947,530 + ABCV34_RS04340 0.19 -0.1
947,531 - ABCV34_RS04340 0.19 -0.4
947,531 - ABCV34_RS04340 0.19 -0.1
947,532 + ABCV34_RS04340 0.20 +2.5
947,662 + ABCV34_RS04340 0.33 -1.2
947,801 + ABCV34_RS04340 0.47 -1.2
947,801 + ABCV34_RS04340 0.47 +0.4
947,801 + ABCV34_RS04340 0.47 -0.9
947,801 + ABCV34_RS04340 0.47 -1.5
947,801 + ABCV34_RS04340 0.47 +0.4
947,802 - ABCV34_RS04340 0.47 -0.4
947,802 - ABCV34_RS04340 0.47 -0.9
947,802 - ABCV34_RS04340 0.47 -1.1
947,802 - ABCV34_RS04340 0.47 -1.1
947,900 - ABCV34_RS04340 0.57 -0.6
947,989 + ABCV34_RS04340 0.66 +1.9
947,989 + ABCV34_RS04340 0.66 -0.4
947,990 - ABCV34_RS04340 0.67 -0.9
948,048 + ABCV34_RS04340 0.73 -0.9
948,049 - ABCV34_RS04340 0.73 -3.3
948,107 - ABCV34_RS04340 0.79 +0.4
948,208 + ABCV34_RS04340 0.89 -0.1

Or see this region's nucleotide sequence