Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS03060 and ABCV34_RS03065 are separated by 84 nucleotides ABCV34_RS03065 and ABCV34_RS03070 are separated by 133 nucleotides ABCV34_RS03070 and ABCV34_RS03075 overlap by 28 nucleotides ABCV34_RS03075 and ABCV34_RS03080 are separated by 9 nucleotides
ABCV34_RS03060: ABCV34_RS03060 - argininosuccinate lyase, at 684,389 to 685,807
_RS03060
ABCV34_RS03065: ABCV34_RS03065 - P-II family nitrogen regulator, at 685,892 to 686,230
_RS03065
ABCV34_RS03070: ABCV34_RS03070 - pseudouridine synthase, at 686,364 to 687,095
_RS03070
ABCV34_RS03075: ABCV34_RS03075 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 687,068 to 688,048
_RS03075
ABCV34_RS03080: ABCV34_RS03080 - glycerophosphodiester phosphodiesterase, at 688,058 to 688,825
_RS03080
Position (kb) 686
687
688 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.821 kb on + strand at 685.822 kb on - strand at 685.822 kb on - strand at 685.822 kb on - strand at 685.822 kb on - strand at 685.822 kb on - strand at 685.843 kb on + strand at 685.844 kb on - strand at 685.844 kb on - strand at 685.844 kb on - strand at 685.845 kb on + strand at 685.845 kb on + strand at 685.846 kb on - strand at 685.846 kb on - strand at 685.846 kb on - strand at 685.846 kb on - strand at 685.848 kb on + strand at 685.848 kb on + strand at 685.849 kb on - strand at 685.849 kb on - strand at 685.849 kb on - strand at 686.027 kb on + strand, within ABCV34_RS03065 at 686.028 kb on - strand, within ABCV34_RS03065 at 686.028 kb on - strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.042 kb on + strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.043 kb on - strand, within ABCV34_RS03065 at 686.228 kb on + strand at 686.228 kb on + strand at 686.228 kb on + strand at 686.228 kb on + strand at 686.229 kb on - strand at 686.229 kb on - strand at 686.229 kb on - strand at 686.229 kb on - strand at 686.229 kb on - strand at 686.307 kb on + strand at 686.308 kb on - strand at 686.348 kb on + strand at 686.348 kb on + strand at 686.348 kb on + strand at 686.348 kb on + strand at 686.349 kb on - strand at 686.349 kb on - strand at 686.349 kb on - strand at 686.349 kb on - strand at 686.349 kb on - strand at 686.349 kb on - strand at 686.433 kb on + strand at 686.433 kb on + strand at 686.433 kb on + strand at 686.433 kb on + strand at 686.433 kb on + strand at 686.434 kb on - strand at 686.434 kb on - strand at 686.434 kb on - strand at 686.434 kb on - strand at 686.583 kb on + strand, within ABCV34_RS03070 at 686.583 kb on + strand, within ABCV34_RS03070 at 686.583 kb on + strand, within ABCV34_RS03070 at 686.584 kb on - strand, within ABCV34_RS03070 at 686.584 kb on - strand, within ABCV34_RS03070 at 686.584 kb on - strand, within ABCV34_RS03070 at 686.811 kb on + strand, within ABCV34_RS03070 at 686.811 kb on + strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.812 kb on - strand, within ABCV34_RS03070 at 686.975 kb on + strand, within ABCV34_RS03070 at 686.975 kb on + strand, within ABCV34_RS03070 at 686.975 kb on + strand, within ABCV34_RS03070 at 686.976 kb on - strand, within ABCV34_RS03070 at 686.976 kb on - strand, within ABCV34_RS03070 at 686.976 kb on - strand, within ABCV34_RS03070 at 686.976 kb on - strand, within ABCV34_RS03070 at 686.976 kb on - strand, within ABCV34_RS03070 at 687.023 kb on + strand at 687.023 kb on + strand at 687.023 kb on + strand at 687.023 kb on + strand at 687.069 kb on + strand at 687.070 kb on - strand at 687.070 kb on - strand at 687.070 kb on - strand at 687.070 kb on - strand at 687.534 kb on + strand, within ABCV34_RS03075 at 687.534 kb on + strand, within ABCV34_RS03075 at 687.534 kb on + strand, within ABCV34_RS03075 at 687.535 kb on - strand, within ABCV34_RS03075 at 687.535 kb on - strand, within ABCV34_RS03075 at 687.807 kb on + strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.808 kb on - strand, within ABCV34_RS03075 at 687.906 kb on + strand, within ABCV34_RS03075 at 687.906 kb on + strand, within ABCV34_RS03075 at 687.906 kb on + strand, within ABCV34_RS03075 at 687.906 kb on + strand, within ABCV34_RS03075 at 687.907 kb on - strand, within ABCV34_RS03075 at 687.907 kb on - strand, within ABCV34_RS03075 at 687.942 kb on + strand, within ABCV34_RS03075 at 687.949 kb on + strand, within ABCV34_RS03075 at 687.950 kb on - strand, within ABCV34_RS03075 at 687.955 kb on + strand at 687.955 kb on + strand at 687.955 kb on + strand at 687.956 kb on - strand at 687.956 kb on - strand at 687.956 kb on - strand at 687.966 kb on + strand at 687.966 kb on + strand at 687.967 kb on - strand at 687.998 kb on + strand at 687.998 kb on + strand at 687.999 kb on - strand at 687.999 kb on - strand at 688.038 kb on + strand at 688.039 kb on - strand at 688.042 kb on + strand at 688.042 kb on + strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand at 688.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 685,821 + -2.4 685,821 + -1.2 685,821 + -2.3 685,821 + -2.1 685,821 + -0.2 685,821 + -2.3 685,821 + +3.2 685,821 + -0.1 685,822 - -1.0 685,822 - -2.8 685,822 - -2.1 685,822 - -2.1 685,822 - -4.6 685,843 + -1.5 685,844 - -2.5 685,844 - -3.0 685,844 - +2.0 685,845 + +0.7 685,845 + +1.0 685,846 - +0.3 685,846 - -1.5 685,846 - -4.1 685,846 - -1.2 685,848 + -3.6 685,848 + -1.9 685,849 - +0.3 685,849 - -3.3 685,849 - -2.8 686,027 + ABCV34_RS03065 0.40 -4.1 686,028 - ABCV34_RS03065 0.40 -1.4 686,028 - ABCV34_RS03065 0.40 +2.6 686,042 + ABCV34_RS03065 0.44 -0.7 686,042 + ABCV34_RS03065 0.44 +0.1 686,042 + ABCV34_RS03065 0.44 -2.5 686,042 + ABCV34_RS03065 0.44 -0.0 686,042 + ABCV34_RS03065 0.44 -0.5 686,042 + ABCV34_RS03065 0.44 -1.3 686,043 - ABCV34_RS03065 0.45 +1.5 686,043 - ABCV34_RS03065 0.45 +0.3 686,043 - ABCV34_RS03065 0.45 -0.2 686,043 - ABCV34_RS03065 0.45 -1.6 686,043 - ABCV34_RS03065 0.45 -1.0 686,043 - ABCV34_RS03065 0.45 -2.4 686,043 - ABCV34_RS03065 0.45 -4.0 686,043 - ABCV34_RS03065 0.45 -0.9 686,228 + -2.1 686,228 + -0.2 686,228 + -1.6 686,228 + -0.5 686,229 - +0.2 686,229 - -0.9 686,229 - -1.9 686,229 - +1.0 686,229 - -0.4 686,307 + -1.6 686,308 - -2.0 686,348 + +1.0 686,348 + +1.0 686,348 + +0.4 686,348 + -2.3 686,349 - -1.0 686,349 - -2.3 686,349 - -2.7 686,349 - -1.5 686,349 - -3.9 686,349 - -1.0 686,433 + +0.3 686,433 + -1.9 686,433 + -2.8 686,433 + +1.0 686,433 + -3.4 686,434 - -0.9 686,434 - -2.4 686,434 - -1.0 686,434 - -1.2 686,583 + ABCV34_RS03070 0.30 -0.2 686,583 + ABCV34_RS03070 0.30 -0.7 686,583 + ABCV34_RS03070 0.30 -0.3 686,584 - ABCV34_RS03070 0.30 -3.7 686,584 - ABCV34_RS03070 0.30 -1.9 686,584 - ABCV34_RS03070 0.30 +2.0 686,811 + ABCV34_RS03070 0.61 -1.9 686,811 + ABCV34_RS03070 0.61 -0.6 686,812 - ABCV34_RS03070 0.61 -1.6 686,812 - ABCV34_RS03070 0.61 +1.3 686,812 - ABCV34_RS03070 0.61 -1.3 686,812 - ABCV34_RS03070 0.61 -2.9 686,812 - ABCV34_RS03070 0.61 +0.2 686,812 - ABCV34_RS03070 0.61 -3.7 686,975 + ABCV34_RS03070 0.83 +0.0 686,975 + ABCV34_RS03070 0.83 +0.1 686,975 + ABCV34_RS03070 0.83 -0.2 686,976 - ABCV34_RS03070 0.84 -3.2 686,976 - ABCV34_RS03070 0.84 -1.3 686,976 - ABCV34_RS03070 0.84 +0.4 686,976 - ABCV34_RS03070 0.84 -0.5 686,976 - ABCV34_RS03070 0.84 -0.4 687,023 + -1.4 687,023 + -3.1 687,023 + +1.6 687,023 + +1.8 687,069 + +0.1 687,070 - -1.0 687,070 - +0.4 687,070 - +0.2 687,070 - -1.5 687,534 + ABCV34_RS03075 0.48 +1.2 687,534 + ABCV34_RS03075 0.48 -4.0 687,534 + ABCV34_RS03075 0.48 -1.6 687,535 - ABCV34_RS03075 0.48 -1.6 687,535 - ABCV34_RS03075 0.48 -1.8 687,807 + ABCV34_RS03075 0.75 -0.4 687,808 - ABCV34_RS03075 0.75 -0.6 687,808 - ABCV34_RS03075 0.75 -2.7 687,808 - ABCV34_RS03075 0.75 -0.3 687,808 - ABCV34_RS03075 0.75 -0.7 687,808 - ABCV34_RS03075 0.75 -1.6 687,808 - ABCV34_RS03075 0.75 -0.1 687,906 + ABCV34_RS03075 0.85 -1.3 687,906 + ABCV34_RS03075 0.85 -2.1 687,906 + ABCV34_RS03075 0.85 -3.7 687,906 + ABCV34_RS03075 0.85 +1.1 687,907 - ABCV34_RS03075 0.86 -2.9 687,907 - ABCV34_RS03075 0.86 -1.5 687,942 + ABCV34_RS03075 0.89 -0.9 687,949 + ABCV34_RS03075 0.90 -0.5 687,950 - ABCV34_RS03075 0.90 -1.9 687,955 + -1.7 687,955 + -3.8 687,955 + -1.8 687,956 - -3.9 687,956 - -2.9 687,956 - -1.2 687,966 + -3.0 687,966 + +1.4 687,967 - -1.6 687,998 + +1.1 687,998 + -2.1 687,999 - -0.4 687,999 - -2.0 688,038 + +0.7 688,039 - -1.0 688,042 + -2.1 688,042 + +0.5 688,043 - -1.4 688,043 - -1.8 688,043 - -1.4 688,043 - -3.3 688,043 - +0.5 688,043 - -1.2 688,043 - -3.9 688,043 - -1.6 688,043 - -3.7 688,043 - -3.8 688,043 - +0.2
Or see this region's nucleotide sequence