Experiment: Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABCV34_RS01210 and ABCV34_RS01215 are separated by 10 nucleotides ABCV34_RS01215 and ABCV34_RS01220 are separated by 38 nucleotides ABCV34_RS01220 and ABCV34_RS01225 are separated by 114 nucleotides ABCV34_RS01225 and ABCV34_RS01230 are separated by 4 nucleotides
ABCV34_RS01210: ABCV34_RS01210 - acetolactate synthase 3 catalytic subunit, at 254,791 to 256,539
_RS01210
ABCV34_RS01215: ABCV34_RS01215 - acetolactate synthase small subunit, at 256,550 to 257,041
_RS01215
ABCV34_RS01220: ABCV34_RS01220 - ketol-acid reductoisomerase, at 257,080 to 258,096
_RS01220
ABCV34_RS01225: ABCV34_RS01225 - phosphatidylserine decarboxylase, at 258,211 to 258,891
_RS01225
ABCV34_RS01230: ABCV34_RS01230 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 258,896 to 259,657
_RS01230
Position (kb) 257
258
259 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 256.286 kb on - strand, within ABCV34_RS01210 at 256.297 kb on + strand, within ABCV34_RS01210 at 256.309 kb on + strand, within ABCV34_RS01210 at 256.309 kb on + strand, within ABCV34_RS01210 at 256.309 kb on + strand, within ABCV34_RS01210 at 256.310 kb on - strand, within ABCV34_RS01210 at 256.310 kb on - strand, within ABCV34_RS01210 at 256.310 kb on - strand, within ABCV34_RS01210 at 256.310 kb on - strand, within ABCV34_RS01210 at 256.352 kb on - strand, within ABCV34_RS01210 at 256.352 kb on - strand, within ABCV34_RS01210 at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.423 kb on + strand at 256.424 kb on - strand at 256.424 kb on - strand at 256.424 kb on - strand at 256.424 kb on - strand at 256.424 kb on - strand at 256.632 kb on - strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.907 kb on + strand, within ABCV34_RS01215 at 256.908 kb on - strand, within ABCV34_RS01215 at 256.936 kb on + strand, within ABCV34_RS01215 at 257.037 kb on + strand at 257.037 kb on + strand at 257.037 kb on + strand at 257.037 kb on + strand at 257.037 kb on + strand at 257.037 kb on + strand at 257.037 kb on + strand at 257.038 kb on - strand at 257.038 kb on - strand at 257.038 kb on - strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.039 kb on + strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.040 kb on - strand at 257.059 kb on + strand at 257.059 kb on + strand at 257.059 kb on + strand at 257.059 kb on + strand at 257.060 kb on - strand at 257.060 kb on - strand at 257.072 kb on + strand at 257.072 kb on + strand at 257.092 kb on + strand at 257.093 kb on - strand at 257.345 kb on - strand, within ABCV34_RS01220 at 257.417 kb on - strand, within ABCV34_RS01220 at 257.493 kb on + strand, within ABCV34_RS01220 at 257.493 kb on + strand, within ABCV34_RS01220 at 257.494 kb on - strand, within ABCV34_RS01220 at 257.494 kb on - strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.497 kb on + strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.498 kb on - strand, within ABCV34_RS01220 at 257.499 kb on + strand, within ABCV34_RS01220 at 257.499 kb on + strand, within ABCV34_RS01220 at 257.499 kb on + strand, within ABCV34_RS01220 at 257.499 kb on + strand, within ABCV34_RS01220 at 257.500 kb on - strand, within ABCV34_RS01220 at 257.500 kb on - strand, within ABCV34_RS01220 at 257.579 kb on - strand, within ABCV34_RS01220 at 257.579 kb on - strand, within ABCV34_RS01220 at 257.749 kb on + strand, within ABCV34_RS01220 at 257.846 kb on - strand, within ABCV34_RS01220 at 257.846 kb on - strand, within ABCV34_RS01220 at 257.854 kb on + strand, within ABCV34_RS01220 at 257.854 kb on + strand, within ABCV34_RS01220 at 257.854 kb on + strand, within ABCV34_RS01220 at 257.854 kb on + strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.855 kb on - strand, within ABCV34_RS01220 at 257.936 kb on - strand, within ABCV34_RS01220 at 258.094 kb on + strand at 258.094 kb on + strand at 258.094 kb on + strand at 258.094 kb on + strand at 258.094 kb on + strand at 258.094 kb on + strand at 258.095 kb on - strand at 258.095 kb on - strand at 258.095 kb on - strand at 258.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=20250415-U1-SZ1-UF; TreatmentDuration=24hrs; Outgrowth=R2A_Kan10_Lactate remove 256,286 - ABCV34_RS01210 0.85 -1.0 256,297 + ABCV34_RS01210 0.86 -2.0 256,309 + ABCV34_RS01210 0.87 -3.2 256,309 + ABCV34_RS01210 0.87 -3.5 256,309 + ABCV34_RS01210 0.87 -1.4 256,310 - ABCV34_RS01210 0.87 +1.0 256,310 - ABCV34_RS01210 0.87 -1.8 256,310 - ABCV34_RS01210 0.87 +0.3 256,310 - ABCV34_RS01210 0.87 -3.2 256,352 - ABCV34_RS01210 0.89 -1.4 256,352 - ABCV34_RS01210 0.89 -1.1 256,423 + -4.0 256,423 + -3.8 256,423 + -1.8 256,423 + -1.4 256,423 + -1.2 256,423 + -3.6 256,423 + -0.8 256,423 + +0.2 256,423 + -0.8 256,424 - -2.6 256,424 - +0.4 256,424 - +1.6 256,424 - -2.5 256,424 - -1.4 256,632 - ABCV34_RS01215 0.17 -1.8 256,907 + ABCV34_RS01215 0.73 -0.4 256,907 + ABCV34_RS01215 0.73 -0.4 256,907 + ABCV34_RS01215 0.73 -6.0 256,907 + ABCV34_RS01215 0.73 -1.0 256,907 + ABCV34_RS01215 0.73 -3.0 256,907 + ABCV34_RS01215 0.73 -0.0 256,907 + ABCV34_RS01215 0.73 +0.1 256,907 + ABCV34_RS01215 0.73 +0.1 256,907 + ABCV34_RS01215 0.73 -0.9 256,908 - ABCV34_RS01215 0.73 -4.6 256,936 + ABCV34_RS01215 0.78 -1.0 257,037 + +3.5 257,037 + +2.0 257,037 + -1.4 257,037 + -0.4 257,037 + -0.6 257,037 + +1.0 257,037 + -1.2 257,038 - -2.7 257,038 - -2.8 257,038 - -0.6 257,039 + -0.2 257,039 + -1.9 257,039 + -3.1 257,039 + -1.5 257,039 + -1.8 257,039 + -2.6 257,039 + +0.1 257,039 + -1.6 257,039 + -2.9 257,039 + +0.4 257,039 + -1.5 257,039 + -2.5 257,040 - -1.6 257,040 - -3.5 257,040 - -1.8 257,040 - -0.9 257,040 - -1.0 257,040 - -1.5 257,040 - -3.2 257,040 - -2.9 257,040 - -1.5 257,040 - -1.5 257,040 - -2.5 257,040 - -1.4 257,040 - -0.9 257,059 + +2.0 257,059 + -1.7 257,059 + +0.6 257,059 + -0.2 257,060 - -1.1 257,060 - -0.0 257,072 + -2.7 257,072 + +0.4 257,092 + -0.6 257,093 - +0.4 257,345 - ABCV34_RS01220 0.26 -0.6 257,417 - ABCV34_RS01220 0.33 -2.4 257,493 + ABCV34_RS01220 0.41 -1.9 257,493 + ABCV34_RS01220 0.41 -0.2 257,494 - ABCV34_RS01220 0.41 +1.0 257,494 - ABCV34_RS01220 0.41 -0.0 257,497 + ABCV34_RS01220 0.41 -0.0 257,497 + ABCV34_RS01220 0.41 -1.1 257,497 + ABCV34_RS01220 0.41 -1.0 257,497 + ABCV34_RS01220 0.41 -2.5 257,497 + ABCV34_RS01220 0.41 -1.6 257,497 + ABCV34_RS01220 0.41 -1.4 257,497 + ABCV34_RS01220 0.41 -0.3 257,497 + ABCV34_RS01220 0.41 -2.4 257,497 + ABCV34_RS01220 0.41 +0.4 257,497 + ABCV34_RS01220 0.41 -3.6 257,497 + ABCV34_RS01220 0.41 -1.2 257,497 + ABCV34_RS01220 0.41 +0.3 257,497 + ABCV34_RS01220 0.41 +2.0 257,497 + ABCV34_RS01220 0.41 +1.7 257,497 + ABCV34_RS01220 0.41 -1.4 257,497 + ABCV34_RS01220 0.41 -2.0 257,498 - ABCV34_RS01220 0.41 -1.2 257,498 - ABCV34_RS01220 0.41 -0.8 257,498 - ABCV34_RS01220 0.41 -0.6 257,498 - ABCV34_RS01220 0.41 -3.2 257,498 - ABCV34_RS01220 0.41 +0.2 257,498 - ABCV34_RS01220 0.41 +2.3 257,498 - ABCV34_RS01220 0.41 -1.9 257,498 - ABCV34_RS01220 0.41 -3.2 257,498 - ABCV34_RS01220 0.41 -0.8 257,498 - ABCV34_RS01220 0.41 +0.9 257,498 - ABCV34_RS01220 0.41 -0.1 257,498 - ABCV34_RS01220 0.41 +0.7 257,498 - ABCV34_RS01220 0.41 -1.2 257,498 - ABCV34_RS01220 0.41 +2.0 257,498 - ABCV34_RS01220 0.41 +0.0 257,498 - ABCV34_RS01220 0.41 +2.0 257,498 - ABCV34_RS01220 0.41 -3.1 257,498 - ABCV34_RS01220 0.41 +0.4 257,498 - ABCV34_RS01220 0.41 -0.0 257,498 - ABCV34_RS01220 0.41 -1.6 257,498 - ABCV34_RS01220 0.41 -2.9 257,498 - ABCV34_RS01220 0.41 -0.4 257,498 - ABCV34_RS01220 0.41 +1.3 257,498 - ABCV34_RS01220 0.41 -1.2 257,498 - ABCV34_RS01220 0.41 -2.9 257,498 - ABCV34_RS01220 0.41 -1.9 257,499 + ABCV34_RS01220 0.41 +1.0 257,499 + ABCV34_RS01220 0.41 -0.6 257,499 + ABCV34_RS01220 0.41 +1.2 257,499 + ABCV34_RS01220 0.41 -1.4 257,500 - ABCV34_RS01220 0.41 -2.1 257,500 - ABCV34_RS01220 0.41 -1.4 257,579 - ABCV34_RS01220 0.49 -1.6 257,579 - ABCV34_RS01220 0.49 +1.0 257,749 + ABCV34_RS01220 0.66 +1.0 257,846 - ABCV34_RS01220 0.75 +0.7 257,846 - ABCV34_RS01220 0.75 -1.4 257,854 + ABCV34_RS01220 0.76 -0.4 257,854 + ABCV34_RS01220 0.76 -0.0 257,854 + ABCV34_RS01220 0.76 +0.4 257,854 + ABCV34_RS01220 0.76 -2.8 257,855 - ABCV34_RS01220 0.76 +3.0 257,855 - ABCV34_RS01220 0.76 +1.0 257,855 - ABCV34_RS01220 0.76 +3.5 257,855 - ABCV34_RS01220 0.76 -1.6 257,855 - ABCV34_RS01220 0.76 +4.4 257,855 - ABCV34_RS01220 0.76 +1.0 257,936 - ABCV34_RS01220 0.84 -0.4 258,094 + -2.9 258,094 + -1.8 258,094 + -3.3 258,094 + -0.3 258,094 + -3.2 258,094 + -0.2 258,095 - +0.0 258,095 - -1.9 258,095 - -3.8 258,207 + -3.9
Or see this region's nucleotide sequence