Experiment: LB agar plate coculture with Mixed culture; Pseudomonas plecoglossicida TBS_049 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection=direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00416 and MPMX20_00417 overlap by 25 nucleotides MPMX20_00417 and MPMX20_00418 are separated by 113 nucleotides MPMX20_00418 and MPMX20_00419 are separated by 116 nucleotides
MPMX20_00416: MPMX20_00416 - Thiol:disulfide interchange protein DsbD, at 431,688 to 433,391
_00416
MPMX20_00417: MPMX20_00417 - Divalent-cation tolerance protein CutA, at 433,367 to 433,690
_00417
MPMX20_00418: MPMX20_00418 - Anaerobic C4-dicarboxylate transporter DcuA, at 433,804 to 435,105
_00418
MPMX20_00419: MPMX20_00419 - Aspartate ammonia-lyase, at 435,222 to 436,658
_00419
Position (kb) 433
434
435
436 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 432.823 kb on + strand, within MPMX20_00416 at 432.842 kb on + strand, within MPMX20_00416 at 432.891 kb on + strand, within MPMX20_00416 at 432.899 kb on - strand, within MPMX20_00416 at 432.909 kb on - strand, within MPMX20_00416 at 432.909 kb on - strand, within MPMX20_00416 at 432.909 kb on - strand, within MPMX20_00416 at 432.928 kb on - strand, within MPMX20_00416 at 432.935 kb on - strand, within MPMX20_00416 at 433.008 kb on + strand, within MPMX20_00416 at 433.016 kb on - strand, within MPMX20_00416 at 433.024 kb on + strand, within MPMX20_00416 at 433.029 kb on + strand, within MPMX20_00416 at 433.029 kb on + strand, within MPMX20_00416 at 433.037 kb on - strand, within MPMX20_00416 at 433.037 kb on - strand, within MPMX20_00416 at 433.096 kb on - strand, within MPMX20_00416 at 433.155 kb on + strand, within MPMX20_00416 at 433.155 kb on + strand, within MPMX20_00416 at 433.155 kb on + strand, within MPMX20_00416 at 433.163 kb on - strand, within MPMX20_00416 at 433.166 kb on + strand, within MPMX20_00416 at 433.171 kb on + strand, within MPMX20_00416 at 433.171 kb on + strand, within MPMX20_00416 at 433.179 kb on - strand, within MPMX20_00416 at 433.179 kb on - strand, within MPMX20_00416 at 433.192 kb on + strand, within MPMX20_00416 at 433.192 kb on + strand, within MPMX20_00416 at 433.192 kb on + strand, within MPMX20_00416 at 433.192 kb on + strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.200 kb on - strand, within MPMX20_00416 at 433.202 kb on + strand, within MPMX20_00416 at 433.213 kb on + strand, within MPMX20_00416 at 433.223 kb on - strand at 433.250 kb on + strand at 433.351 kb on + strand at 433.356 kb on - strand at 433.359 kb on - strand at 433.370 kb on + strand at 433.380 kb on + strand at 433.388 kb on - strand at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.472 kb on + strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.480 kb on - strand, within MPMX20_00417 at 433.511 kb on - strand, within MPMX20_00417 at 433.514 kb on + strand, within MPMX20_00417 at 433.514 kb on + strand, within MPMX20_00417 at 433.561 kb on - strand, within MPMX20_00417 at 433.628 kb on + strand, within MPMX20_00417 at 433.628 kb on + strand, within MPMX20_00417 at 433.633 kb on + strand, within MPMX20_00417 at 433.636 kb on - strand, within MPMX20_00417 at 433.636 kb on - strand, within MPMX20_00417 at 433.664 kb on - strand at 433.706 kb on + strand at 433.927 kb on - strand at 433.937 kb on + strand, within MPMX20_00418 at 433.947 kb on + strand, within MPMX20_00418 at 433.947 kb on + strand, within MPMX20_00418 at 433.982 kb on - strand, within MPMX20_00418 at 433.982 kb on - strand, within MPMX20_00418 at 434.000 kb on + strand, within MPMX20_00418 at 434.013 kb on + strand, within MPMX20_00418 at 434.013 kb on + strand, within MPMX20_00418 at 434.041 kb on + strand, within MPMX20_00418 at 434.041 kb on + strand, within MPMX20_00418 at 434.041 kb on + strand, within MPMX20_00418 at 434.049 kb on - strand, within MPMX20_00418 at 434.064 kb on + strand, within MPMX20_00418 at 434.072 kb on - strand, within MPMX20_00418 at 434.077 kb on - strand, within MPMX20_00418 at 434.105 kb on + strand, within MPMX20_00418 at 434.146 kb on + strand, within MPMX20_00418 at 434.146 kb on + strand, within MPMX20_00418 at 434.146 kb on + strand, within MPMX20_00418 at 434.154 kb on - strand, within MPMX20_00418 at 434.184 kb on + strand, within MPMX20_00418 at 434.189 kb on + strand, within MPMX20_00418 at 434.213 kb on - strand, within MPMX20_00418 at 434.213 kb on - strand, within MPMX20_00418 at 434.240 kb on - strand, within MPMX20_00418 at 434.282 kb on + strand, within MPMX20_00418 at 434.290 kb on - strand, within MPMX20_00418 at 434.340 kb on + strand, within MPMX20_00418 at 434.358 kb on + strand, within MPMX20_00418 at 434.366 kb on - strand, within MPMX20_00418 at 434.392 kb on - strand, within MPMX20_00418 at 434.397 kb on - strand, within MPMX20_00418 at 434.427 kb on - strand, within MPMX20_00418 at 434.427 kb on - strand, within MPMX20_00418 at 434.558 kb on - strand, within MPMX20_00418 at 434.573 kb on - strand, within MPMX20_00418 at 434.627 kb on + strand, within MPMX20_00418 at 434.627 kb on + strand, within MPMX20_00418 at 434.631 kb on + strand, within MPMX20_00418 at 434.671 kb on - strand, within MPMX20_00418 at 434.727 kb on + strand, within MPMX20_00418 at 434.729 kb on + strand, within MPMX20_00418 at 434.759 kb on - strand, within MPMX20_00418 at 434.769 kb on - strand, within MPMX20_00418 at 434.773 kb on + strand, within MPMX20_00418 at 434.807 kb on + strand, within MPMX20_00418 at 434.815 kb on - strand, within MPMX20_00418 at 434.911 kb on - strand, within MPMX20_00418 at 434.919 kb on - strand, within MPMX20_00418 at 434.967 kb on + strand, within MPMX20_00418 at 434.978 kb on + strand at 434.978 kb on + strand at 434.978 kb on + strand at 434.986 kb on - strand at 434.986 kb on - strand at 434.997 kb on + strand at 434.997 kb on + strand at 435.005 kb on - strand at 435.012 kb on + strand at 435.020 kb on - strand at 435.044 kb on + strand at 435.046 kb on + strand at 435.054 kb on - strand at 435.103 kb on + strand at 435.103 kb on + strand at 435.103 kb on + strand at 435.103 kb on - strand at 435.103 kb on - strand at 435.112 kb on - strand at 435.196 kb on + strand at 435.206 kb on + strand at 435.211 kb on + strand at 435.211 kb on + strand at 435.214 kb on - strand at 435.214 kb on - strand at 435.214 kb on - strand at 435.219 kb on - strand at 435.315 kb on - strand at 435.327 kb on + strand at 435.388 kb on + strand, within MPMX20_00419 at 435.410 kb on + strand, within MPMX20_00419 at 435.438 kb on + strand, within MPMX20_00419 at 435.444 kb on + strand, within MPMX20_00419 at 435.444 kb on + strand, within MPMX20_00419 at 435.468 kb on + strand, within MPMX20_00419 at 435.503 kb on + strand, within MPMX20_00419 at 435.514 kb on + strand, within MPMX20_00419 at 435.606 kb on + strand, within MPMX20_00419 at 435.611 kb on + strand, within MPMX20_00419 at 435.621 kb on - strand, within MPMX20_00419 at 435.632 kb on + strand, within MPMX20_00419 at 435.632 kb on + strand, within MPMX20_00419 at 435.640 kb on - strand, within MPMX20_00419 at 435.640 kb on - strand, within MPMX20_00419 at 435.663 kb on - strand, within MPMX20_00419 at 435.693 kb on - strand, within MPMX20_00419 at 435.737 kb on - strand, within MPMX20_00419 at 435.754 kb on - strand, within MPMX20_00419 at 435.759 kb on - strand, within MPMX20_00419 at 435.767 kb on + strand, within MPMX20_00419 at 435.822 kb on - strand, within MPMX20_00419 at 435.884 kb on + strand, within MPMX20_00419 at 435.892 kb on - strand, within MPMX20_00419 at 435.892 kb on - strand, within MPMX20_00419 at 435.893 kb on - strand, within MPMX20_00419 at 435.939 kb on + strand, within MPMX20_00419 at 435.947 kb on - strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 436.096 kb on - strand, within MPMX20_00419
Per-strain Table
Position Strand Gene LocusTag Fraction LB agar plate coculture with Mixed culture; Pseudomonas plecoglossicida TBS_049 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection=direct remove 432,823 + MPMX20_00416 0.67 +0.3 432,842 + MPMX20_00416 0.68 +0.9 432,891 + MPMX20_00416 0.71 -0.3 432,899 - MPMX20_00416 0.71 +1.6 432,909 - MPMX20_00416 0.72 +1.0 432,909 - MPMX20_00416 0.72 +0.2 432,909 - MPMX20_00416 0.72 +1.0 432,928 - MPMX20_00416 0.73 +1.5 432,935 - MPMX20_00416 0.73 -0.8 433,008 + MPMX20_00416 0.77 -0.8 433,016 - MPMX20_00416 0.78 +0.3 433,024 + MPMX20_00416 0.78 -1.2 433,029 + MPMX20_00416 0.79 -0.1 433,029 + MPMX20_00416 0.79 +0.1 433,037 - MPMX20_00416 0.79 +1.2 433,037 - MPMX20_00416 0.79 -0.7 433,096 - MPMX20_00416 0.83 -1.3 433,155 + MPMX20_00416 0.86 +0.8 433,155 + MPMX20_00416 0.86 +0.8 433,155 + MPMX20_00416 0.86 -0.6 433,163 - MPMX20_00416 0.87 +0.6 433,166 + MPMX20_00416 0.87 -0.4 433,171 + MPMX20_00416 0.87 -0.0 433,171 + MPMX20_00416 0.87 -1.3 433,179 - MPMX20_00416 0.88 -2.9 433,179 - MPMX20_00416 0.88 +0.1 433,192 + MPMX20_00416 0.88 +0.6 433,192 + MPMX20_00416 0.88 +0.8 433,192 + MPMX20_00416 0.88 +1.0 433,192 + MPMX20_00416 0.88 -0.3 433,200 - MPMX20_00416 0.89 -1.8 433,200 - MPMX20_00416 0.89 -0.6 433,200 - MPMX20_00416 0.89 +0.7 433,200 - MPMX20_00416 0.89 -1.1 433,200 - MPMX20_00416 0.89 +0.7 433,200 - MPMX20_00416 0.89 +0.8 433,202 + MPMX20_00416 0.89 -0.6 433,213 + MPMX20_00416 0.89 -1.8 433,223 - -1.0 433,250 + -0.9 433,351 + -1.9 433,356 - -0.7 433,359 - +0.3 433,370 + -3.5 433,380 + -1.2 433,388 - +0.8 433,472 + MPMX20_00417 0.32 -0.0 433,472 + MPMX20_00417 0.32 -3.1 433,472 + MPMX20_00417 0.32 +0.6 433,472 + MPMX20_00417 0.32 +0.5 433,472 + MPMX20_00417 0.32 +0.4 433,472 + MPMX20_00417 0.32 +1.4 433,472 + MPMX20_00417 0.32 -0.7 433,472 + MPMX20_00417 0.32 +1.2 433,472 + MPMX20_00417 0.32 +1.0 433,480 - MPMX20_00417 0.35 -0.6 433,480 - MPMX20_00417 0.35 -0.1 433,480 - MPMX20_00417 0.35 -1.8 433,480 - MPMX20_00417 0.35 +1.0 433,480 - MPMX20_00417 0.35 +0.7 433,480 - MPMX20_00417 0.35 +0.7 433,480 - MPMX20_00417 0.35 +1.1 433,480 - MPMX20_00417 0.35 +1.3 433,480 - MPMX20_00417 0.35 +0.6 433,480 - MPMX20_00417 0.35 -1.1 433,480 - MPMX20_00417 0.35 +0.8 433,480 - MPMX20_00417 0.35 +1.3 433,480 - MPMX20_00417 0.35 -2.4 433,511 - MPMX20_00417 0.44 +1.2 433,514 + MPMX20_00417 0.45 +1.2 433,514 + MPMX20_00417 0.45 -0.4 433,561 - MPMX20_00417 0.60 +0.3 433,628 + MPMX20_00417 0.81 -2.2 433,628 + MPMX20_00417 0.81 +0.2 433,633 + MPMX20_00417 0.82 -2.1 433,636 - MPMX20_00417 0.83 +1.3 433,636 - MPMX20_00417 0.83 +1.9 433,664 - +0.4 433,706 + +0.3 433,927 - -1.3 433,937 + MPMX20_00418 0.10 +0.7 433,947 + MPMX20_00418 0.11 +1.0 433,947 + MPMX20_00418 0.11 -4.7 433,982 - MPMX20_00418 0.14 -0.5 433,982 - MPMX20_00418 0.14 +0.2 434,000 + MPMX20_00418 0.15 -0.4 434,013 + MPMX20_00418 0.16 +0.7 434,013 + MPMX20_00418 0.16 +0.5 434,041 + MPMX20_00418 0.18 -0.6 434,041 + MPMX20_00418 0.18 -1.4 434,041 + MPMX20_00418 0.18 -0.0 434,049 - MPMX20_00418 0.19 +1.4 434,064 + MPMX20_00418 0.20 +1.0 434,072 - MPMX20_00418 0.21 -2.1 434,077 - MPMX20_00418 0.21 +0.3 434,105 + MPMX20_00418 0.23 -1.3 434,146 + MPMX20_00418 0.26 -1.0 434,146 + MPMX20_00418 0.26 -0.2 434,146 + MPMX20_00418 0.26 -1.0 434,154 - MPMX20_00418 0.27 +1.3 434,184 + MPMX20_00418 0.29 -0.9 434,189 + MPMX20_00418 0.30 -0.8 434,213 - MPMX20_00418 0.31 -0.8 434,213 - MPMX20_00418 0.31 +0.2 434,240 - MPMX20_00418 0.33 -0.8 434,282 + MPMX20_00418 0.37 +0.6 434,290 - MPMX20_00418 0.37 -1.6 434,340 + MPMX20_00418 0.41 +0.2 434,358 + MPMX20_00418 0.43 +0.1 434,366 - MPMX20_00418 0.43 -1.3 434,392 - MPMX20_00418 0.45 +0.4 434,397 - MPMX20_00418 0.46 +0.2 434,427 - MPMX20_00418 0.48 -1.0 434,427 - MPMX20_00418 0.48 -2.1 434,558 - MPMX20_00418 0.58 -0.2 434,573 - MPMX20_00418 0.59 -0.7 434,627 + MPMX20_00418 0.63 -3.2 434,627 + MPMX20_00418 0.63 -1.5 434,631 + MPMX20_00418 0.64 -0.8 434,671 - MPMX20_00418 0.67 -0.1 434,727 + MPMX20_00418 0.71 +0.7 434,729 + MPMX20_00418 0.71 -0.0 434,759 - MPMX20_00418 0.73 -3.0 434,769 - MPMX20_00418 0.74 -0.6 434,773 + MPMX20_00418 0.74 +0.4 434,807 + MPMX20_00418 0.77 -2.5 434,815 - MPMX20_00418 0.78 +0.7 434,911 - MPMX20_00418 0.85 -2.6 434,919 - MPMX20_00418 0.86 +1.0 434,967 + MPMX20_00418 0.89 -0.0 434,978 + +1.0 434,978 + -2.7 434,978 + +0.9 434,986 - -0.8 434,986 - -0.5 434,997 + -4.2 434,997 + +0.2 435,005 - -0.8 435,012 + +0.6 435,020 - -1.0 435,044 + +0.1 435,046 + +0.0 435,054 - -1.3 435,103 + -1.7 435,103 + +0.3 435,103 + -1.4 435,103 - -1.8 435,103 - -0.8 435,112 - -1.8 435,196 + -1.6 435,206 + +0.1 435,211 + -1.5 435,211 + -1.5 435,214 - -2.7 435,214 - -1.4 435,214 - -1.1 435,219 - -0.7 435,315 - -0.6 435,327 + +0.4 435,388 + MPMX20_00419 0.12 -2.9 435,410 + MPMX20_00419 0.13 -3.8 435,438 + MPMX20_00419 0.15 +2.0 435,444 + MPMX20_00419 0.15 -2.7 435,444 + MPMX20_00419 0.15 -0.0 435,468 + MPMX20_00419 0.17 -4.0 435,503 + MPMX20_00419 0.20 -0.8 435,514 + MPMX20_00419 0.20 -3.0 435,606 + MPMX20_00419 0.27 -0.3 435,611 + MPMX20_00419 0.27 +0.3 435,621 - MPMX20_00419 0.28 +1.0 435,632 + MPMX20_00419 0.29 -2.0 435,632 + MPMX20_00419 0.29 -1.3 435,640 - MPMX20_00419 0.29 +1.4 435,640 - MPMX20_00419 0.29 -1.5 435,663 - MPMX20_00419 0.31 -1.3 435,693 - MPMX20_00419 0.33 +0.6 435,737 - MPMX20_00419 0.36 -0.0 435,754 - MPMX20_00419 0.37 -1.2 435,759 - MPMX20_00419 0.37 -1.3 435,767 + MPMX20_00419 0.38 +1.0 435,822 - MPMX20_00419 0.42 -0.1 435,884 + MPMX20_00419 0.46 -1.0 435,892 - MPMX20_00419 0.47 -1.2 435,892 - MPMX20_00419 0.47 +0.2 435,893 - MPMX20_00419 0.47 +0.5 435,939 + MPMX20_00419 0.50 -2.0 435,947 - MPMX20_00419 0.50 -0.3 435,989 + MPMX20_00419 0.53 +0.7 435,989 + MPMX20_00419 0.53 -2.7 435,989 + MPMX20_00419 0.53 -1.2 435,997 - MPMX20_00419 0.54 -0.8 435,997 - MPMX20_00419 0.54 -2.6 435,997 - MPMX20_00419 0.54 -0.8 435,997 - MPMX20_00419 0.54 -0.6 435,997 - MPMX20_00419 0.54 -1.9 436,096 - MPMX20_00419 0.61 -0.6
Or see this region's nucleotide sequence