Experiment: mutant library grown in Supernatant; Pseudomonas frederiksbergensis TBS_004 grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00071 and MPMX20_00072 are separated by 102 nucleotides MPMX20_00072 and MPMX20_00073 are separated by 99 nucleotides MPMX20_00073 and MPMX20_00074 are separated by 117 nucleotides MPMX20_00074 and MPMX20_00075 are separated by 77 nucleotides
MPMX20_00071: MPMX20_00071 - hypothetical protein, at 72,030 to 72,989
_00071
MPMX20_00072: MPMX20_00072 - 5-hydroxyisourate hydrolase, at 73,092 to 73,502
_00072
MPMX20_00073: MPMX20_00073 - HTH-type transcriptional regulator MlrA, at 73,602 to 74,327
_00073
MPMX20_00074: MPMX20_00074 - Outer membrane lipoprotein Blc, at 74,445 to 74,969
_00074
MPMX20_00075: MPMX20_00075 - hypothetical protein, at 75,047 to 76,093
_00075
Position (kb) 73
74
75 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 72.604 kb on - strand, within MPMX20_00071 at 72.608 kb on + strand, within MPMX20_00071 at 72.785 kb on + strand, within MPMX20_00071 at 72.814 kb on - strand, within MPMX20_00071 at 72.826 kb on - strand, within MPMX20_00071 at 72.840 kb on + strand, within MPMX20_00071 at 72.840 kb on + strand, within MPMX20_00071 at 72.851 kb on + strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.880 kb on + strand, within MPMX20_00071 at 72.884 kb on + strand, within MPMX20_00071 at 72.884 kb on + strand, within MPMX20_00071 at 72.884 kb on + strand, within MPMX20_00071 at 72.888 kb on - strand, within MPMX20_00071 at 72.894 kb on + strand at 72.902 kb on - strand at 72.984 kb on - strand at 73.169 kb on + strand, within MPMX20_00072 at 73.177 kb on - strand, within MPMX20_00072 at 73.200 kb on + strand, within MPMX20_00072 at 73.229 kb on - strand, within MPMX20_00072 at 73.231 kb on - strand, within MPMX20_00072 at 73.247 kb on + strand, within MPMX20_00072 at 73.256 kb on + strand, within MPMX20_00072 at 73.256 kb on + strand, within MPMX20_00072 at 73.259 kb on - strand, within MPMX20_00072 at 73.277 kb on - strand, within MPMX20_00072 at 73.323 kb on - strand, within MPMX20_00072 at 73.376 kb on + strand, within MPMX20_00072 at 73.378 kb on - strand, within MPMX20_00072 at 73.411 kb on - strand, within MPMX20_00072 at 73.430 kb on + strand, within MPMX20_00072 at 73.454 kb on + strand, within MPMX20_00072 at 73.454 kb on + strand, within MPMX20_00072 at 73.455 kb on + strand, within MPMX20_00072 at 73.455 kb on + strand, within MPMX20_00072 at 73.462 kb on - strand at 73.463 kb on - strand at 73.481 kb on + strand at 73.512 kb on - strand at 73.519 kb on - strand at 73.530 kb on + strand at 73.616 kb on + strand at 73.624 kb on - strand at 73.624 kb on - strand at 73.673 kb on + strand at 73.673 kb on + strand at 73.673 kb on + strand at 73.681 kb on - strand, within MPMX20_00073 at 73.681 kb on - strand, within MPMX20_00073 at 73.681 kb on - strand, within MPMX20_00073 at 73.681 kb on - strand, within MPMX20_00073 at 73.704 kb on - strand, within MPMX20_00073 at 73.779 kb on + strand, within MPMX20_00073 at 73.787 kb on - strand, within MPMX20_00073 at 73.787 kb on - strand, within MPMX20_00073 at 73.787 kb on - strand, within MPMX20_00073 at 73.821 kb on - strand, within MPMX20_00073 at 73.865 kb on + strand, within MPMX20_00073 at 73.865 kb on + strand, within MPMX20_00073 at 73.887 kb on + strand, within MPMX20_00073 at 73.927 kb on - strand, within MPMX20_00073 at 73.930 kb on + strand, within MPMX20_00073 at 73.975 kb on + strand, within MPMX20_00073 at 73.985 kb on + strand, within MPMX20_00073 at 73.985 kb on + strand, within MPMX20_00073 at 73.990 kb on + strand, within MPMX20_00073 at 73.990 kb on + strand, within MPMX20_00073 at 73.990 kb on + strand, within MPMX20_00073 at 73.990 kb on + strand, within MPMX20_00073 at 73.990 kb on + strand, within MPMX20_00073 at 73.993 kb on - strand, within MPMX20_00073 at 73.996 kb on + strand, within MPMX20_00073 at 73.998 kb on - strand, within MPMX20_00073 at 73.998 kb on - strand, within MPMX20_00073 at 73.998 kb on - strand, within MPMX20_00073 at 73.998 kb on - strand, within MPMX20_00073 at 74.089 kb on - strand, within MPMX20_00073 at 74.108 kb on + strand, within MPMX20_00073 at 74.128 kb on + strand, within MPMX20_00073 at 74.184 kb on + strand, within MPMX20_00073 at 74.203 kb on + strand, within MPMX20_00073 at 74.208 kb on - strand, within MPMX20_00073 at 74.247 kb on + strand, within MPMX20_00073 at 74.261 kb on + strand at 74.264 kb on - strand at 74.264 kb on - strand at 74.322 kb on + strand at 74.323 kb on + strand at 74.331 kb on - strand at 74.334 kb on + strand at 74.342 kb on - strand at 74.392 kb on + strand at 74.392 kb on + strand at 74.400 kb on - strand at 74.402 kb on + strand at 74.402 kb on + strand at 74.402 kb on + strand at 74.402 kb on + strand at 74.402 kb on + strand at 74.460 kb on + strand at 74.460 kb on + strand at 74.468 kb on - strand at 74.468 kb on - strand at 74.482 kb on + strand at 74.515 kb on - strand, within MPMX20_00074 at 74.524 kb on + strand, within MPMX20_00074 at 74.525 kb on + strand, within MPMX20_00074 at 74.619 kb on - strand, within MPMX20_00074 at 74.685 kb on - strand, within MPMX20_00074 at 74.785 kb on - strand, within MPMX20_00074 at 74.788 kb on + strand, within MPMX20_00074 at 74.796 kb on - strand, within MPMX20_00074 at 74.826 kb on + strand, within MPMX20_00074 at 74.893 kb on + strand, within MPMX20_00074 at 74.898 kb on + strand, within MPMX20_00074 at 74.917 kb on - strand at 74.917 kb on - strand at 74.946 kb on - strand at 74.951 kb on + strand at 74.951 kb on + strand at 74.951 kb on - strand at 74.956 kb on + strand at 74.956 kb on + strand at 74.956 kb on + strand at 74.956 kb on + strand at 74.956 kb on + strand at 74.959 kb on - strand at 74.964 kb on - strand at 74.991 kb on + strand at 75.008 kb on + strand at 75.147 kb on - strand at 75.171 kb on + strand, within MPMX20_00075 at 75.201 kb on + strand, within MPMX20_00075 at 75.224 kb on + strand, within MPMX20_00075 at 75.243 kb on - strand, within MPMX20_00075 at 75.243 kb on - strand, within MPMX20_00075 at 75.316 kb on + strand, within MPMX20_00075
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Pseudomonas frederiksbergensis TBS_004 grown in LB remove 72,604 - MPMX20_00071 0.60 +0.0 72,608 + MPMX20_00071 0.60 +1.3 72,785 + MPMX20_00071 0.79 +0.5 72,814 - MPMX20_00071 0.82 +0.7 72,826 - MPMX20_00071 0.83 +0.4 72,840 + MPMX20_00071 0.84 +0.5 72,840 + MPMX20_00071 0.84 +0.3 72,851 + MPMX20_00071 0.86 +1.1 72,859 - MPMX20_00071 0.86 +0.5 72,859 - MPMX20_00071 0.86 -0.7 72,859 - MPMX20_00071 0.86 -0.3 72,859 - MPMX20_00071 0.86 +0.2 72,859 - MPMX20_00071 0.86 -0.6 72,880 + MPMX20_00071 0.89 +0.1 72,884 + MPMX20_00071 0.89 -0.8 72,884 + MPMX20_00071 0.89 +0.4 72,884 + MPMX20_00071 0.89 +0.1 72,888 - MPMX20_00071 0.89 -1.2 72,894 + -1.0 72,902 - -0.3 72,984 - +0.7 73,169 + MPMX20_00072 0.19 -0.5 73,177 - MPMX20_00072 0.21 -0.7 73,200 + MPMX20_00072 0.26 +0.2 73,229 - MPMX20_00072 0.33 -1.3 73,231 - MPMX20_00072 0.34 +0.3 73,247 + MPMX20_00072 0.38 +0.6 73,256 + MPMX20_00072 0.40 +0.2 73,256 + MPMX20_00072 0.40 +0.0 73,259 - MPMX20_00072 0.41 -0.0 73,277 - MPMX20_00072 0.45 +0.8 73,323 - MPMX20_00072 0.56 -0.7 73,376 + MPMX20_00072 0.69 -0.1 73,378 - MPMX20_00072 0.70 +0.1 73,411 - MPMX20_00072 0.78 +0.7 73,430 + MPMX20_00072 0.82 +0.3 73,454 + MPMX20_00072 0.88 +0.3 73,454 + MPMX20_00072 0.88 +2.0 73,455 + MPMX20_00072 0.88 +0.0 73,455 + MPMX20_00072 0.88 -0.4 73,462 - -0.1 73,463 - +1.8 73,481 + -0.4 73,512 - -0.5 73,519 - -0.6 73,530 + +0.2 73,616 + -1.0 73,624 - -2.3 73,624 - -2.2 73,673 + -2.3 73,673 + -0.0 73,673 + -1.7 73,681 - MPMX20_00073 0.11 +0.2 73,681 - MPMX20_00073 0.11 -1.9 73,681 - MPMX20_00073 0.11 -1.2 73,681 - MPMX20_00073 0.11 -2.0 73,704 - MPMX20_00073 0.14 -1.7 73,779 + MPMX20_00073 0.24 -1.0 73,787 - MPMX20_00073 0.25 -3.6 73,787 - MPMX20_00073 0.25 -1.2 73,787 - MPMX20_00073 0.25 -0.9 73,821 - MPMX20_00073 0.30 -2.1 73,865 + MPMX20_00073 0.36 -1.7 73,865 + MPMX20_00073 0.36 -0.6 73,887 + MPMX20_00073 0.39 -3.9 73,927 - MPMX20_00073 0.45 -0.3 73,930 + MPMX20_00073 0.45 -1.7 73,975 + MPMX20_00073 0.51 -1.9 73,985 + MPMX20_00073 0.53 -1.9 73,985 + MPMX20_00073 0.53 -2.0 73,990 + MPMX20_00073 0.53 -1.5 73,990 + MPMX20_00073 0.53 -0.9 73,990 + MPMX20_00073 0.53 -3.5 73,990 + MPMX20_00073 0.53 -0.7 73,990 + MPMX20_00073 0.53 -1.5 73,993 - MPMX20_00073 0.54 -2.3 73,996 + MPMX20_00073 0.54 +1.5 73,998 - MPMX20_00073 0.55 -1.0 73,998 - MPMX20_00073 0.55 -1.3 73,998 - MPMX20_00073 0.55 -1.4 73,998 - MPMX20_00073 0.55 -2.9 74,089 - MPMX20_00073 0.67 -3.5 74,108 + MPMX20_00073 0.70 -1.6 74,128 + MPMX20_00073 0.72 +0.4 74,184 + MPMX20_00073 0.80 -0.5 74,203 + MPMX20_00073 0.83 -0.2 74,208 - MPMX20_00073 0.83 -2.3 74,247 + MPMX20_00073 0.89 -2.8 74,261 + -3.5 74,264 - -1.9 74,264 - +0.1 74,322 + -1.9 74,323 + -0.9 74,331 - -0.3 74,334 + -1.8 74,342 - +0.5 74,392 + -1.0 74,392 + +0.4 74,400 - -0.7 74,402 + +1.5 74,402 + +0.8 74,402 + +1.7 74,402 + +0.4 74,402 + -0.0 74,460 + -0.2 74,460 + -0.9 74,468 - +0.6 74,468 - -0.2 74,482 + +0.2 74,515 - MPMX20_00074 0.13 -1.3 74,524 + MPMX20_00074 0.15 -0.8 74,525 + MPMX20_00074 0.15 +0.5 74,619 - MPMX20_00074 0.33 +0.3 74,685 - MPMX20_00074 0.46 -0.6 74,785 - MPMX20_00074 0.65 -1.3 74,788 + MPMX20_00074 0.65 +1.0 74,796 - MPMX20_00074 0.67 +0.0 74,826 + MPMX20_00074 0.73 +0.1 74,893 + MPMX20_00074 0.85 -0.4 74,898 + MPMX20_00074 0.86 +1.0 74,917 - +0.3 74,917 - -0.8 74,946 - -0.2 74,951 + -0.6 74,951 + -3.0 74,951 - +0.5 74,956 + +0.8 74,956 + +0.0 74,956 + -0.4 74,956 + +1.2 74,956 + +0.5 74,959 - -0.3 74,964 - +0.1 74,991 + +0.4 75,008 + +1.2 75,147 - -1.4 75,171 + MPMX20_00075 0.12 -0.0 75,201 + MPMX20_00075 0.15 +0.2 75,224 + MPMX20_00075 0.17 -0.0 75,243 - MPMX20_00075 0.19 +0.3 75,243 - MPMX20_00075 0.19 -1.9 75,316 + MPMX20_00075 0.26 -0.5
Or see this region's nucleotide sequence