Experiment: PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_003220 and TX73_003225 are separated by 186 nucleotides TX73_003225 and TX73_003230 are separated by 331 nucleotides
TX73_003220: TX73_003220 - methionyl-tRNA formyltransferase, at 689,455 to 690,387
_003220
TX73_003225: TX73_003225 - tRNA pseudouridine(38-40) synthase TruA, at 690,574 to 691,311
_003225
TX73_003230: TX73_003230 - succinyl-diaminopimelate desuccinylase, at 691,643 to 692,806
_003230
Position (kb)
690
691
692 Strain fitness (log2 ratio)
-2
-1
0
1 at 690.369 kb on + strand at 690.369 kb on + strand at 690.369 kb on + strand at 690.377 kb on - strand at 690.382 kb on + strand at 690.382 kb on + strand at 690.382 kb on + strand at 690.382 kb on + strand at 690.382 kb on + strand at 690.382 kb on + strand at 690.387 kb on + strand at 690.390 kb on - strand at 690.390 kb on - strand at 690.394 kb on + strand at 690.395 kb on - strand at 690.400 kb on + strand at 690.400 kb on + strand at 690.408 kb on - strand at 690.408 kb on - strand at 690.408 kb on - strand at 690.408 kb on - strand at 690.408 kb on - strand at 690.408 kb on - strand at 690.417 kb on + strand at 690.481 kb on - strand at 690.503 kb on - strand at 690.503 kb on - strand at 690.503 kb on - strand at 690.503 kb on - strand at 690.513 kb on + strand at 690.544 kb on + strand at 690.645 kb on + strand at 690.645 kb on + strand at 690.647 kb on + strand at 690.655 kb on - strand, within TX73_003225 at 690.700 kb on - strand, within TX73_003225 at 690.812 kb on + strand, within TX73_003225 at 690.830 kb on + strand, within TX73_003225 at 690.845 kb on + strand, within TX73_003225 at 690.851 kb on - strand, within TX73_003225 at 690.877 kb on - strand, within TX73_003225 at 690.882 kb on + strand, within TX73_003225 at 690.890 kb on - strand, within TX73_003225 at 690.890 kb on - strand, within TX73_003225 at 690.937 kb on - strand, within TX73_003225 at 690.957 kb on + strand, within TX73_003225 at 690.996 kb on + strand, within TX73_003225 at 691.011 kb on + strand, within TX73_003225 at 691.012 kb on + strand, within TX73_003225 at 691.090 kb on - strand, within TX73_003225 at 691.156 kb on + strand, within TX73_003225 at 691.275 kb on + strand at 691.284 kb on + strand at 691.331 kb on + strand at 691.331 kb on + strand at 691.339 kb on - strand at 691.339 kb on - strand at 691.339 kb on - strand at 691.339 kb on - strand at 691.341 kb on - strand at 691.377 kb on + strand at 691.448 kb on - strand at 691.465 kb on + strand at 691.575 kb on - strand at 691.579 kb on + strand at 691.587 kb on + strand at 691.587 kb on - strand at 691.587 kb on - strand at 691.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark remove 690,369 + -0.7 690,369 + +0.9 690,369 + -0.4 690,377 - +0.3 690,382 + +0.2 690,382 + +1.2 690,382 + -0.4 690,382 + +0.0 690,382 + +0.7 690,382 + -1.5 690,387 + +0.9 690,390 - +0.1 690,390 - +0.5 690,394 + +0.1 690,395 - -0.3 690,400 + +0.1 690,400 + +1.1 690,408 - +0.1 690,408 - -0.6 690,408 - +0.4 690,408 - +0.4 690,408 - -0.4 690,408 - +0.0 690,417 + +0.5 690,481 - -0.3 690,503 - -0.0 690,503 - +1.0 690,503 - +0.2 690,503 - -0.1 690,513 + -0.5 690,544 + -0.4 690,645 + +0.5 690,645 + -1.9 690,647 + -1.2 690,655 - TX73_003225 0.11 +1.3 690,700 - TX73_003225 0.17 +0.2 690,812 + TX73_003225 0.32 -1.3 690,830 + TX73_003225 0.35 -0.3 690,845 + TX73_003225 0.37 -0.6 690,851 - TX73_003225 0.38 -1.2 690,877 - TX73_003225 0.41 -0.8 690,882 + TX73_003225 0.42 -2.5 690,890 - TX73_003225 0.43 -1.2 690,890 - TX73_003225 0.43 +0.5 690,937 - TX73_003225 0.49 -0.9 690,957 + TX73_003225 0.52 -0.8 690,996 + TX73_003225 0.57 -1.3 691,011 + TX73_003225 0.59 -1.3 691,012 + TX73_003225 0.59 -1.0 691,090 - TX73_003225 0.70 -0.8 691,156 + TX73_003225 0.79 -2.6 691,275 + -2.1 691,284 + -1.8 691,331 + +0.8 691,331 + +0.3 691,339 - -0.3 691,339 - -0.1 691,339 - +0.3 691,339 - -0.5 691,341 - -0.1 691,377 + +0.3 691,448 - +1.0 691,465 + +0.3 691,575 - +0.1 691,579 + +0.2 691,587 + +0.3 691,587 - -0.9 691,587 - +0.0 691,644 - -0.1
Or see this region's nucleotide sequence